Allergenic Differences among Pollens of Three &lt;i&gt;Salsola&lt;/i&gt; Species

Ferrer, A.; Larramendi, Carlos H.; Huertas, A.J.; Pagán, Juan Antonio; Andreu, Carlos Manuel Fernández; García-Abujeta, José Luis; López-Matas, M. Ángeles; Carnés, Jerónimo

doi:10.1159/000242357

Cited by 8 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…found that the frequency of sensitization to Timothy grass pollens in males in series was 56%, while in females, it was 70.6%, with no significant difference in between [ 28 ]. Pollens sensitizations were the most representative aeroallergen sensitization (44.9%) in Portugal and in southwest Germany [ 29 , 30 ]. Nevertheless, it was 87.1% in Kuwait [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Food and Aeroallergen Sensitization in IgE -Mediated Asthma in Egypt

Ali¹

2021

TORMJ

View full text Add to dashboard Cite

Purpose: Identifying the distribution of allergens is valuable to the effective diagnosis and treatment of allergic disease. So, our aim is to explore the sensitization of food and aeroallergens in Egyptian patients with atopic asthma. Methods: Cross-sectional study recruited 268 Egyptian patients with atopic asthma. Asthmatic patients were assessed by the enzyme allegro sorbent test (EAST) method for specific IgE to a panel of 19 common regional inhaled allergens and 15 food allergens. Results and Discussion: One hundred percent of the patients were sensitive to at least one allergen. Allergy to food allergens only was 2.9%; inhaled allergens only were 26.2% and both were70.9%. Fungi (62%) were the most frequent sensitizing aeroallergen amongst our asthmatic patients, followed by the pollen allergens (42.5%) and house dust mites (HDMs) (26%). Cows’ milk (30.5%) was the most frequent sensitizing food amongst our asthmatic patients, followed by eggs (22.4%) and fish (21.6%). Mono-sensitized patients accounted for 6.7% of all cases, while polysensitized was 93.3%. Moderate and severe asthma showed a significantly higher frequency of polysensitization compared to mild asthma. Conclusion: Fungi and cow's milk are the chief sensitizing allergens in Egyptian patients with atopic asthma. This study represents the first report of sensitization in atopic adult asthma using a large extract panel in Upper Egypt.

show abstract

Section: Discussionmentioning

confidence: 99%

Food and Aeroallergen Sensitization in IgE -Mediated Asthma in Egypt

Ali¹

2021

TORMJ

View full text Add to dashboard Cite

show abstract

“…Although S. kali is considered the main allergenic pollen from Salsola sp., and their pollen allergens have been studied extensively in the last years [6], other Salsola sp. like S. oppositifolia and S. vermiculata have recently shown an important allergenic capacity [33]. …”

Section: Discussionmentioning

confidence: 99%

A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from Salsola kali Pollen for Allergy Diagnosis

Mas

Boissy

Monsalve

et al. 2015

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: The incidence of Amaranthaceae pollen allergy has increased due to the desertification occurring in many countries. In some regions of Spain, Salsola kali is the main cause of pollinosis, at almost the same level as olive and grass pollen. Sal k 1 - the sensitization marker of S. kali pollinosis - is used in clinical diagnosis, but is purified at a low yield from pollen. We aimed to produce a recombinant (r)Sal k 1 able to span the structural and immunological properties of the natural isoforms from pollen, and validate its potential use for diagnosis. Methods: Specific cDNA was amplified by PCR, cloned into the pET41b vector and used to transform BL21 (DE3) Escherichia coli cells. Immunoblotting, ELISA, basophil activation and skin-prick tests were used to validate the recombinant protein against Sal k 1 isolated from pollen. Sera and blood cells from S. kali pollen-sensitized patients and specific monoclonal and polyclonal antisera were used. Results: rSal k 1 was produced in bacteria with a yield of 7.5 mg/l of cell culture. The protein was purified to homogeneity and structural and immunologically validated against the natural form. rSal k 1 exhibited a higher IgE cross-reactivity with plant-derived food extracts such as peanut, almond or tomato than with pollen sources such as Platanus acerifolia and Oleaceae members. Conclusions: rSal k 1 expressed in bacteria retains intact structural and immunological properties in comparison to the pollen-derived allergen. It spans the immunological properties of most of the isoforms found in pollen, and it might substitute natural Sal k 1 in clinical diagnosis.

show abstract

“…Botanical similarities, coincident pollination periods and mainly small, spherical and periporate pollen grains do not allow discrimination of pollen grains from different species by optical microscopy. In addition, only C. album and S. kali [6], and recently S. oppositifolia [16] on the Mediterranean coast of Spain and A. retroflexus in Iran [17], have been associated with allergic symptoms. Previous published studies have described a significant time lag between pollen counts and symptom scores in S. kali -monosensitized patients [15].…”

Section: Discussionmentioning

confidence: 99%

“…Recently published papers have suggested that other Chenopodiaceae species, which pollinate in different periods, could also contribute to allergic symptoms [16,17]. …”

Section: Introductionmentioning

confidence: 99%

Assessing Degree of Flowering Implicates Multiple Chenopodiaceae/Amaranthaceae Species in Allergy

Ferrer

Carnés²,

Rojas-Hijazo

et al. 2011

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: IgE-mediated sensitization to the Chenopodiaceae/Amaranthaceae families is a cause of allergic symptoms in arid areas. Salsola kali and Chenopodium album are considered the main species responsible; however, there is a discrepancy between the pollination period of these two plants and clinical symptoms. The objectives of this study were to identify new Chenopodiaceae/Amaranthaceae members with sensitization capacity and to correlate symptoms, pollen counts and degree of flowering of different species. Methods: A total of 37 individuals monosensitized to S. kali and C. album were included in the study. All patients recorded daily symptom scores between May and October 2007. Extracts from Chenopodium (album, vulvaria and murale), Salsola (kali, vermiculata, and oppositifolia), Bassia scoparia, Atriplex (patula and halimus) and Amaranthus (deflexus and muricatus) were manufactured and used in skin prick tests (SPTs). Protein content and IgE binding were assessed for each extract. Pollen counts and degree of flowering (based on the Orshan specific semiquantitative method) were assessed weekly. Results: Symptom scores demonstrated a positive correlation with pollen counts even outside the pollination period of S. kali. Positive SPTs were obtained with all 11 species tested, which showed common proteins with IgE-binding capacity. Different species flowered at different times during the pollen season. Conclusion: Different taxonomically related species of Chenopodiaceae/Amaranthaceae can induce allergic sensitization and should be considered for use in diagnosis and treatment. Degree of flowering is a complementary method for assessing pollination that could be used for botanical families with indistinguishable pollen grains.

show abstract

Allergenic Differences among Pollens of Three <i>Salsola</i> Species

Cited by 8 publications

References 25 publications

Food and Aeroallergen Sensitization in IgE -Mediated Asthma in Egypt

Food and Aeroallergen Sensitization in IgE -Mediated Asthma in Egypt

A Recombinant Sal k 1 Isoform as an Alternative to the Polymorphic Allergen from <b><i>Salsola kali</i></b> Pollen for Allergy Diagnosis

Assessing Degree of Flowering Implicates Multiple <i>Chenopodiaceae/Amaranthaceae</i> Species in Allergy

Contact Info

Product

Resources

About