Allergen-specific T-cell Response in Patients with Phenytoin Hypersensitivity; Simultaneous Analysis of Proliferation and Cytokine Production by Carboxyfluorescein Succinimidyl Ester (CFSE) Dilution Assay

Tsuge, Ikuya; Okumura, Akihisa; Kondo, Yasuto; Itomi, Seiko; Kakami, Michiko; Kawamura, Makiko; Nakajima, Yoshiyuki; Komatsubara, Ryo; Urisu, Atsuo

doi:10.2332/allergolint.o-06-457

Cited by 38 publications

(32 citation statements)

References 18 publications

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“…El estudio de Tsuge I et al 31 fue el úni-co que empleó el estudio de linfoproliferación, y halló una sensibilidad de 100% (IC95:93-100), especificidad de 91% (IC95:69-100), CP+ 11 (IC95%:2-71) e Índice J de Younden 0,91(IC95%:0,74-1,08). En la evaluación de la validez diagnós-tica de la prueba de activación de basófilos se halló una sensibilidad global de 78% (IC95%:74%-81%) con un rango de 48%-1 0 0 % .…”

Section: Resultsunclassified

Evaluación de técnicas inmunológicas in vitro para el diagnóstico de alergias: metanálisis 2000-2012

Cuervo-Perez¹,

Arango²,

Cardona‐Arias³

2014

Rev. Esp. Salud Publica

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Fundamentos: Las alergias presentan elevada prevalencia, afectan a todos los grupos etarios, generan impactos negativos sobre los sistemas de salud, educativo y económico. Se desconoce la utilidad diagnóstica de las pruebas de tamización. El objetivo del estudio fue evaluar la validez, el desempeño, la seguridad y la efectividad diagnóstica de las técnicas inmunológicas in vitro para alergias.Métodos: Revisión sistemática con metaanálisis. Se aplicó una estrategia de búsqueda de estudios en Pubmed, Sciencedirect y Wiley con los términos de búsqueda activation basophil test, lymphocyte transformation test, especific IgE immunoassay. Período estudiado: 2000-2012. Se determinó la reproducibilidad de la selección, extracción y evaluación de la calidad de los artí-culos. Se calculó sensibilidad, especificidad, cocientes de probabilidad, valores predictivos, proporción de resultados falsos, exactitud, razón de odds, índice J de Youden y curva ROC con los software Meta-DiSc(es) y Epidat 3.0.Resultados: Se incluyeron 18 estudios con 3.520 individuos, 58% enfermos y 42% sanos. La activación de basófilos presentó sensibilidad del 78% (IC95%:74-81), especificidad 95% (IC95%:83-100), cociente de probabilidad positivo 9,9 (IC95%:6,8-14,4) y negativo de 0,20 (IC95%:0,13-0,30), OR diagnóstica de 70,8 (IC95:40,8) y un área bajo la curva de 0,97. En la inmunoglobulina E específica la sensibilidad fue 72% (IC95%:69-75), especificidad 90% (IC95%:88-92), cociente de probabilidad positivo 12,9 (IC95%:4,0-41,6) negativo 0,32 (IC95%:0,23-0,43), OR diagnóstica 41,6 (IC95%:11,6-148,9) y área bajo la curva 0,87.Conclusión: Se evidenció que la activación de basófilos y la IgE especí-fica son pruebas útiles en el diagnóstico de alergias. Background: Allergies have high prevalence, affecting all age groups, generate negative impacts on health, educational and economic systems, and they are unknown the diagnostic utility of screening tests. The objective of the study was to evaluate the validity, performance, safety and diagnostic efficiency of in vitro immunological techniques for allergies, 2000-2012.Methods: Systematic review with meta-analysis. We applied a search strategy studies in PubMed, Sciencedirect and Wiley, with search terms activation basophil test, lymphocyte transformation test, especific IgE immunoassay. We determined the reproducibility of the selection, extraction and quality assessment of articles. We calculated sensitivity, specificity, likelihood ratios, predictive values, proportion of false, accuracy, odds ratio, Youden index J and ROC curve in Meta-DiSc(es) and Epidat 3.0. software. Results:We included 18 studies with 3520 individuals, 58% patients and 42% healthy. Activation of basophils showed sensitivity of 78% (95% CI :74-81), specificity 95% (95% CI: 83-100), positive likelihood ratio 9.9 (95% CI: 6.8 to 14.4) and negative of 0.20 (95% CI = 0.13 to 0.30) a diagnostic OR 70.8 (IC95: 40.2 to 124.8) and area under the curve of 0.97. In specific immunoglobulin E sensitivity was 72% (95% CI: 69-75), specificity 90% (...

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Section: Resultsunclassified

Evaluación de técnicas inmunológicas in vitro para el diagnóstico de alergias: metanálisis 2000-2012

Cuervo-Perez¹,

Arango²,

Cardona‐Arias³

2014

Rev. Esp. Salud Publica

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“…In this study, (1) the proliferation indices of T cells were assayed by CFSE-staining method with flow cytometry [32,33], which showed that the BCG HSP70 gene transfected AML/B-ALL cells significantly enhanced lymphocyte proliferation; (2) cytokine level by ELISA suggested that HSP70-transfected AML/B-ALL cells stimulated autologous T cells to secrete the highest cytokine level (IFN-γ); (3) lactate dehydrogenase (LDH) release assay used to measure killing activity of CTLs [34] showed that the killing rate of CTL in the group of HSP70-transfected AML/B-ALL cells was significantly higher than that of the wide-type AML/B-ALL cells and pDisplay-transfected ones, suggesting that BCG HSP70 gene transfection could significantly enhance the killing activity of autologous CTL on leukemia cells.…”

Section: Discussionmentioning

confidence: 99%

The preparation of leukemia cell vaccine expressing BCG heat shock protein 70 and anti-leukemia effect in vitro

Sun

Wang

et al. 2012

International Immunopharmacology

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“…In previous studies our group has shown that the cells mainly involved at the acute phase of MPE induced by drugs were CD4+ with cytotoxic capabilities (perforin and o Without Stimulation • With Stimulation reaction, peripheral blood and the affected tissue (skin) (3,6), thus demonstrating the importance of Thl cell trafficking with effector functions between these tissues for the development of MPE. Although the involvement of CD4+ T cells and their cytokine secretion has been reported in drug allergy (22)(23), no studies have analysed the involvement of TE and TEM cells in these diseases considering also the influence of chemotactic 'cytokines and regulatory cells in the development of the disease.…”

Section: Discussionmentioning

confidence: 99%

Role of Effector Cells (CCR7⁻CD27⁻) and Effector-Memory Cells (CCR7⁻CD27⁺) in Drug-Induced Maculopapular Exanthema

Fernandez¹,

Torres

Lopez

et al. 2010

Int J Immunopathol Pharmacol

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Maculopapular exanthema (MPE) induced by drugs is a T-cell mediated reaction and effector cells may play an important role in its development. We assessed the effector and cutaneous homing phenotype in peripheral blood cells from allergic patients after drug stimulation. This study included 10 patients and 10 controls. The effector phenotype (CCR7−CD27+/−), chemokine receptors (CCR4 and CCR10), and activation (CD25low) and regulatory markers (CD25high) were measured by flow cytometry in both peripheral blood mononuclear cells (PBMCs) and CD4-T-lymphocytes. Proliferation was determined by 5-(−6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) assay and the migratory capacity by a chemotaxis assay using CCL17 and CCL27. Compared to controls, CCR7−CD27− cells were increased in patients without (p=0.003) and with drug stimulation (p<0.001) and had significantly higher proliferation (p=0.010). CCR10 expression was increased in patients after drug stimulation in total and memory CD27+ T-cells. Lymphocyte migration with CCL27 was higher in patients with drug stimulation (p=0.048), with a decrease in CCR7−CD27− (p<0.0001) and an increase in CCR7−CD27+ (p=0.017). In patients, CD4-T-lymphocytes were significantly activated after drug stimulation (p<0.001). In conclusion, we show that effector memory CD4+ T-cells (CCR7−CD27+) respond specifically to the drug responsible for MPE and confirm previous data about the involvement of CCR10 in cell trafficking to the skin.

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Allergen-specific T-cell Response in Patients with Phenytoin Hypersensitivity; Simultaneous Analysis of Proliferation and Cytokine Production by Carboxyfluorescein Succinimidyl Ester (CFSE) Dilution Assay

Cited by 38 publications

References 18 publications

Evaluación de técnicas inmunológicas in vitro para el diagnóstico de alergias: metanálisis 2000-2012

Evaluación de técnicas inmunológicas in vitro para el diagnóstico de alergias: metanálisis 2000-2012

The preparation of leukemia cell vaccine expressing BCG heat shock protein 70 and anti-leukemia effect in vitro

Role of Effector Cells (CCR7⁻CD27⁻) and Effector-Memory Cells (CCR7⁻CD27⁺) in Drug-Induced Maculopapular Exanthema

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Allergen-specific T-cell Response in Patients with Phenytoin Hypersensitivity; Simultaneous Analysis of Proliferation and Cytokine Production by Carboxyfluorescein Succinimidyl Ester (CFSE) Dilution Assay

Cited by 38 publications

References 18 publications

Evaluación de técnicas inmunológicas in vitro para el diagnóstico de alergias: metanálisis 2000-2012

Evaluación de técnicas inmunológicas in vitro para el diagnóstico de alergias: metanálisis 2000-2012

The preparation of leukemia cell vaccine expressing BCG heat shock protein 70 and anti-leukemia effect in vitro

Role of Effector Cells (CCR7−CD27−) and Effector-Memory Cells (CCR7−CD27+) in Drug-Induced Maculopapular Exanthema

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Product

Resources

About

Role of Effector Cells (CCR7⁻CD27⁻) and Effector-Memory Cells (CCR7⁻CD27⁺) in Drug-Induced Maculopapular Exanthema