Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates

Cabauatan, Clarissa R.; Lupinek, Christian; Scheiblhofer, Sandra; Weiss, Richard; Focke‐Tejkl, Margarete; Bhalla, Prem L.; Singh, Mohan B.; Knight, Paul A.; Hage, Marianne van; Ramos, John Donnie A.; Valenta, Rudolf

doi:10.1016/j.jaci.2013.10.004

Cited by 39 publications

(30 citation statements)

References 9 publications

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“…Determination of sIgE to microarrays of allergen components is a promising tool for molecular diagnosis of allergy [19,20]. Our results confirm that the test instrument can also be affected by interference from CCDs [32][33][34]. In a subsample of alcoholic patients sensitized to CCDs, a uniform pattern of positive sIgE results was observed including-as expected-naturally glycosylated allergen components of plant and insect origin.…”

Section: Discussionsupporting

confidence: 78%

False-Positive Results of Serological Tests for Allergy in Alcoholic Patients

Alvela-Suárez¹,

Campos²,

Carballo³

et al. 2019

J Investig Allergol Clin Immunol

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Background: Alcohol consumption is associated with enhanced T H 2 immune responses. Objective: To investigate the frequency of false-positive results in serological tests for allergy in alcoholic patients. Methods: A total of 138 alcoholic patients consecutively admitted to hospital underwent a panel of allergy tests that included serum total IgE, a multiallergen IgE test (UniCAP Phadiatop), and skin prick tests to relevant aeroallergens in the area, which were considered the standard reference for atopy. In selected cases with positive specific IgE (sIgE) to cross-reactive carbohydrate determinants (CCDs) on ImmunoCAP, we determined sIgE to hymenoptera venom components (ADVIA Centaur) and a microarray of 103 allergen components (ISAC). Results: Increased serum total IgE (>170 IU/mL) was observed in 59/110 (54%) of nonatopic (skin prick test-negative) patients. The result of the multiallergen IgE test was positive in 46 nonatopic patients (42%). This finding was closely associated with high serum concentrations of total IgE and sIgE to CCDs. The vast majority of patients with positive CCD-sIgE showed positivity to glycosylated plant and hymenoptera allergen components on ISAC and ADVIA Centaur. Only 1 out of 26 patients with positive sIgE to CCD and hymenoptera venom developed honeybee venom allergy after a median follow-up of 166 months. Correlations between measurements of sIgE to CCD markers on ImmunoCAP, ADVIA Centaur, and ISAC were imperfect. Conclusions: Serological tests for allergy should be interpreted with caution in alcoholic patients, who frequently have increased levels of total IgE and CCD-sIgE and subsequent positivity of sIgE to glycosylated allergen components, irrespective of the method used.

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Section: Discussionsupporting

confidence: 78%

False-Positive Results of Serological Tests for Allergy in Alcoholic Patients

Alvela-Suárez¹,

Campos²,

Carballo³

et al. 2019

J Investig Allergol Clin Immunol

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“…Patients were subdivided according to the classification of terri-tion patterns can be demonstrated, by means of molecular diagnostic approaches, for grasses or tree pollen allergy in different regions of Spain (13,14) or Italy (15,16,17), thus representing possible loco-regional signatures of sensitization. This has also been demonstrated in other countries, such as Africa and Asia (18,19,20). Italy, located in South-Central Europe, includes a boot-shaped peninsula and two large islands, i.e.…”

Section: Study Cohortmentioning

confidence: 64%

An atlas of IgE sensitization patterns in different Italian areas. A multicenter, cross-sectional study

Scala

Villalta

Uasuf

et al. 2018

Eur Ann Allergy Clin Immunol

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The development of recombinant technology supported the allergy diagnostic work-up in the daily clinical practice, representing a useful tool for epidemiological studies. An atlas of the IgE sensitization profiles found throughout Italy was prepared from a nationwide, multicenter, cross-sectional study. 6052 unselected consecutive individuals, belonging to North-West [NW], North-East [NE], Centre [C], South [S], and Islands subset [Is] were evaluated by means of the ImmunoCAP ISAC test. The top-ranked sensitizations found were Cup a 1 in [C] (58.1%) and [S] (53.6%), Phl p 1 in the North (from 46.1% to 49%), and Cyn d 1 in [Is] (44.2%). High frequency of house dust mite group 2 molecules sensitization was found in [C] (36.9%) and [S] Italy (40.8%), whilst low level of reactivity was recorded in [NW] (20%). Pellitory hypersensitivity was mainly found in [C], [S], and [Is], whilst ragweed Amb a 1 sensitivity was particularly found in [NW] Italy. IgE recognition of PR-10, Profilin, and nsLTP was mutually exclusive in 69.1% of cases, PR-10 reactivity mostly occurring in [NE], Profilin in [NW], and nsLTP molecules recognition mainly recorded in [C] and [S]. Divergent IgE sensitization patterns were found along Italy, possibly linked to the distinct geographical locations, indicating multiplex system IgE analysis as a reliable approach for epidemiological evaluation even in small geographical areas.

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“…An allergen microarray containing 103 allergen molecules has detected a high prevalence of asymptomatic IgE sensitizations to tropical pollen‐derived cross‐reactive carbohydrate determinants . The MeDALL allergen chip contains 176 allergen molecules including seven cross‐reactive carbohydrate determinants against which IgE are likely to be clinically irrelevant .…”

Section: From Allergen‐specific Ige To Ige‐mediated Diseasementioning

confidence: 99%

Are allergic multimorbidities and IgE polysensitization associated with the persistence or re‐occurrence of foetal type 2 signalling? The MeDALL hypothesis

Bousquet

Antó

Wickman

et al. 2015

Allergy

125

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Allergic diseases [asthma, rhinitis and atopic dermatitis (AD)] are complex. They are associated with allergen‐specific IgE and nonallergic mechanisms that may coexist in the same patient. In addition, these diseases tend to cluster and patients present concomitant or consecutive diseases (multimorbidity). IgE sensitization should be considered as a quantitative trait. Important clinical and immunological differences exist between mono‐ and polysensitized subjects. Multimorbidities of allergic diseases share common causal mechanisms that are only partly IgE‐mediated. Persistence of allergic diseases over time is associated with multimorbidity and/or IgE polysensitization. The importance of the family history of allergy may decrease with age. This review puts forward the hypothesis that allergic multimorbidities and IgE polysensitization are associated and related to the persistence or re‐occurrence of foetal type 2 signalling. Asthma, rhinitis and AD are manifestations of a common systemic immune imbalance (mesodermal origin) with specific patterns of remodelling (ectodermal or endodermal origin). This study proposes a new classification of IgE‐mediated allergic diseases that allows the definition of novel phenotypes to (i) better understand genetic and epigenetic mechanisms, (ii) better stratify allergic preschool children for prognosis and (iii) propose novel strategies of treatment and prevention.

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Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates

Cited by 39 publications

References 9 publications

False-Positive Results of Serological Tests for Allergy in Alcoholic Patients

False-Positive Results of Serological Tests for Allergy in Alcoholic Patients

An atlas of IgE sensitization patterns in different Italian areas. A multicenter, cross-sectional study

Are allergic multimorbidities and IgE polysensitization associated with the persistence or re‐occurrence of foetal type 2 signalling? The MeDALL hypothesis

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