Allergen‐induced interleukin‐9 production <i>in vitro</i>: correlation with atopy in human adults and comparison with interleukin‐5 and interleukin‐13

Devos, Stefanie; Cormont, F.; Vrtala, Susanne; Hooghe‐Peters, Elisabeth L.; Pirson, F.; Snick, Jacques Van

doi:10.1111/j.1365-2222.2006.02422.x

Cited by 41 publications

(28 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). Our result is consistent with the report by Devos et al[ 10] which employed two asthmatic donors. Accordingly, we analyzed 6-day cultures upon antigen stimulation in the subsequent experiments.…”

Section: Resultssupporting

confidence: 83%

“…Moreover, IL-9 protein and mRNA levels were well correlated with the number of eosinophils in the BALF [9]. In addition, the amount of IL-9 produced by peripheral blood mononuclear cells (PBMCs) was correlated with the titer of allergen-specific IgE in adult atopic asthmatics [10]. These findings in mice and humans suggest that IL-9 plays a critical role in the pathogenesis of atopic asthma.…”

Section: Introductionmentioning

confidence: 86%

See 1 more Smart Citation

IL-9 Production by Peripheral Blood Mononuclear Cells of Atopic Asthmatics

Umezu-Goto

Kajiyama

Kimura

et al. 2007

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: IL-9 might play a critical role in pathogenesis and development of atopic asthma, but there are few reports on allergen-specific IL-9 production by peripheral blood mononuclear cells (PBMCs) obtained from adult asthmatics. Methods: PBMCs were obtained from adult atopic asthmatics and incubated with Dermatophagoides farinae(Der f) extract for the designated time periods. The resulting supernatants were assayed for IL-9 by specific sandwich ELISA. Results: IL-9 production was detectable on day 2 and reached maximum on day 6 after stimulation of PBMCs with Der f extract. IL-9 production in response to Der f extract increased in a dose-dependent manner. CD2- or CD4-bearing cell depletion completely abolished IL-9 production by PBMCs, while CD8-bearing cell depletion did not affect it. Conclusion: CD4+ lymphocytes are the principal source of IL-9 produced by PBMCs of adult atopic asthmatics.

show abstract

Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 86%

IL-9 Production by Peripheral Blood Mononuclear Cells of Atopic Asthmatics

Umezu-Goto

Kajiyama

Kimura

et al. 2007

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…It acts as a T cell growth factor, enhances IL-4-mediated IgE production by B cells, promotes the survival and activation of eosinophils and mast cells, increases expression of IgER␣, and thereby affects IgE-mediated immunity (12). Increased levels of IL-9 mRNA and IL-9R have been reported in allergic asthma (16,17), suggesting that IL-9 plays an important role in airway inflammation. Because severe RSV bronchiolitis is associated with strong Th2 (i.e., increased IL-4) and weak Th1 (i.e., reduced IFN-␥) responses to RSV infection (18), IL-9 is an attractive candidate for interventional therapy.…”

Section: Il-9 Regulates Pathology During Primary and Memorymentioning

confidence: 99%

IL-9 Regulates Pathology during Primary and Memory Responses to Respiratory Syncytial Virus Infection

Dodd

Lum

Goulding

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

IL-9 is a cytokine of great current interest associated with allergic/Th2 responses. High levels of IL-9 are present in bronchial secretions from infants with respiratory syncytial virus (RSV) bronchiolitis. To test its effects in RSV disease with a Th2 profile, BALB/c mice were vaccinated with recombinant vaccinia virus expressing the RSV G protein. On RSV challenge, immunized mice developed augmented disease characterized by enhanced pulmonary Th2 and local IL-9 production peaking on days 7–10 of RSV infection. Depletion with anti-IL-9 Ab at vaccination or RSV challenge enhanced viral clearance. Depletion only at challenge had no effect on disease severity, whereas depletion at immunization and challenge enhanced Th1 responses, inhibited virus-specific IgG1 production, and enhanced disease severity. By contrast, depletion of IL-9 at immunization boosted IgG2a and inhibited the Th2 response and disease during subsequent infection without a concomitant increase in type 1 cytokines. Adoptive transfer of secondary memory CD4 T cells from the spleens of IL-9-depleted mice into naive recipients replicated many of the effects of depletion, indicating that IL-9 acts via CD4 T cells. Therefore, IL-9 is a previously unknown but key modulator of antiviral immunity, regulating T and B cell responses and having potent and specific effects on viral lung disease.

show abstract

“…IL-9 is a pleiotropic cytokine that promotes T-cell and mast-cell growth and mast-cell accumulation in tissue as well as IgE switching in B cells (1). In line, allergic patients have increased numbers of T cells that produce IL-9 in response to various allergens (2,3). Moreover, Th9 cells alter intestinal epithelial cell functions (4,5) and are key players in antiworm immunity (6).…”

mentioning

confidence: 99%

Human Vδ2 T cells are a major source of interleukin-9

Peters

Häsler

Wesch

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Vδ2Vγ9 T cells are the dominant γδ T-cell subset in human peripheral blood. Vδ2 T cells recognize pyrophosphate molecules derived from microbes or tumor cells; hence, they play a role in antimicrobial and antitumor immunity. TGF-β, together with IL-15, induces a regulatory phenotype in Vδ2 T cells, characterized by forkhead box protein P3 (FoxP3) expression and suppressive activity on CD4 T-cell activation. We performed a genome-wide transcriptome analysis and found that the same conditions (TGF-β plus IL-15) strongly enhanced the expression of additional genes in Vδ2 T cells, including IKAROS family zinc finger 4 (IKZF4; Eos), integrin subunit alpha E (ITGAE; CD103/αEβ7), and IL9. This up-regulation was associated with potent IL-9 production as revealed by flow cytometry and multiplex analysis of cell culture supernatants. In contrast to CD4 and CD8 αβ T cells, γδ T cells did not require IL-4 for induction of intracellular IL-9 expression. Upon antigen restimulation of Vδ2 T cells expanded in vitro in the presence of TGF-β and IL-15, IL-9 was the most abundant among 16 analyzed cytokines and chemokines. IL-9 is a pleiotropic cytokine involved in various (patho)physiological conditions, including allergy and tumor defense, where it can promote antitumor immunity. Given the conspicuous sensitivity of many different tumors to Vδ2 T-cell–mediated killing, the conditions defined here for strong induction of IL-9 might be relevant for the development of Vδ2 T-cell–based immunotherapy.

show abstract

Allergen‐induced interleukin‐9 production in vitro: correlation with atopy in human adults and comparison with interleukin‐5 and interleukin‐13

Abstract: Allergen-triggered IL-9 production in vitro is an excellent marker for atopy in adults given its virtual absence in allergen-stimulated PBMC from non-atopic individuals and its correlation with allergen-specific IgE and asthma.

Cited by 41 publications

References 39 publications

IL-9 Production by Peripheral Blood Mononuclear Cells of Atopic Asthmatics

IL-9 Production by Peripheral Blood Mononuclear Cells of Atopic Asthmatics

IL-9 Regulates Pathology during Primary and Memory Responses to Respiratory Syncytial Virus Infection

Human Vδ2 T cells are a major source of interleukin-9

Contact Info

Product

Resources

About