Allergen‐induced bronchial inflammation in house dust mite‐allergic patients with or without asthma

Lopuhaä, Christa E.; Out, Theo A.; Jansen, Henk M.; Aalberse, Rob C.; Zee, Jaring S. van der

doi:10.1046/j.1365-2222.2002.01542.x

Cited by 35 publications

(28 citation statements)

References 30 publications

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“…The difference in bronchial inflammation between asthma and nonasthmatic rhinitis appeared to be more closely related to indices for neutrophilic inflammation (1993).…”

Section: Commonalities and Differences In Mechanisms Between Rhinitismentioning

confidence: 91%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,826

1,761

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“…The difference in bronchial inflammation between asthma and nonasthmatic rhinitis appeared to be more closely related to indices for neutrophilic inflammation (1993).…”

Section: Commonalities and Differences In Mechanisms Between Rhinitismentioning

confidence: 91%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,826

1,761

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“…Neutrophils are recruited to the airways of human asthmatics and in animal models of asthma after allergen inhalation (38,43,60). Importantly, severe asthma phenotypes are associated with substantial recruitment of neutrophils to the airways (62), suggesting a role of these cells in the pathology.…”

Section: Discussionmentioning

confidence: 99%

IL-4 activates equine neutrophils and induces a mixed inflammatory cytokine expression profile with enhanced neutrophil chemotactic mediator release ex vivo

Lavoie-Lamoureux

Moran

Beauchamp

et al. 2010

American Journal of Physiology-Lung Cellular and Molecular Phys

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Neutrophils are potent contributors to the lung pathophysiological changes occurring in allergic airway inflammation, which typically involve T helper type 2 (Th2) cytokine overexpression. We have previously reported that equine pulmonary endothelial cells are activated by the Th2 cytokine IL-4 and express chemotactic factors for neutrophils after stimulation. We have further explored the possible mechanisms linking Th2-driven inflammation and neutrophilia by studying the effects of recombinant equine IL-4, a prototypical Th2 cytokine, on peripheral blood neutrophils (PBN) isolated from normal animals and from horses with asthmatic airway inflammation (equine heaves). We found that IL-4 induced morphological changes in PBN, dose- and time-dependent expression of IL-8 mRNA, as well as the release of chemotactic factors for neutrophils in culture supernatants. Also, IL-4 induced a mixed inflammatory response in PBN from control and asthmatic-animals with increased expression of proinflammatory IL-8 and TNF-α but a marked inhibition of IL-1β. IL-4 type I receptor (IL-4Rα) and CD23 (FcεRII) expression were also upregulated by IL-4. Importantly, disease as well as chronic antigenic exposure modified gene expression by PBN. Finally, we found that activation of equine neutrophils with IL-4 involved STAT6 phosphorylation and p38 MAPK and phosphatidylinositol 3-kinase (PI3K); the pharmacological inhibitors, SB-203580 and LY-294002, respectively, significantly reversed IL-4-induced gene modulation in PBN. Overall, results from this study add to the link between Th2-driven inflammation and neutrophilia in the equine model and further extend the characterization of IL-4 effects on neutrophils.

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“…1 month after the pre-randomisation allergen challenge may be explained by a slower recovery from the allergen induced increase in bronchial inflammation in the fluticasone group. Bronchial allergen challenge increases levels of inflammatory markers, 25 which may increase the sensitivity to detect differences in levels of inflammatory markers between the treatment groups. On the other hand, it should be noted that the medium to high daily dose of fluticasone resulted in relatively low baseline levels of the inflammatory parameters.…”

Section: Discussionmentioning

confidence: 99%

Adding salmeterol to an inhaled corticosteroid: long term effects on bronchial inflammation in asthma

Koopmans

2006

Thorax

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Background: Addition of the long acting b 2 agonist salmeterol to inhaled corticosteroids leads to better symptomatic asthma control than increasing the dose of inhaled corticosteroids. However, little is known about the long term effects of adding salmeterol on the asthmatic inflammatory process, control of which is considered important for the long term outcome of asthma. Methods: After a 4 week fluticasone run-in period, 54 patients with allergic asthma were randomised to receive twice daily treatment with fluticasone 250 mg with or without salmeterol 50 mg for 1 year in a double blind, parallel group design (total daily dose of fluticasone 500 mg in both treatment groups). Primary outcomes were sputum eosinophil numbers and eosinophil cationic protein concentrations. Secondary outcomes were neutrophil associated sputum parameters and a respiratory membrane permeability marker. The effects on allergen induced changes were determined before and at the end of the treatment period. Results: Adding salmeterol to fluticasone resulted in improved peak expiratory flow, symptom scores, rescue medication usage, and bronchial hyperresponsiveness (p,0.05 for all). There was no sustained effect on sputum cell differential counts and cytokine concentrations during the treatment period or on changes induced by allergen challenge at the end of treatment (p.0.05). However, adding salmeterol significantly reduced sputum ratios of a 2 -macroglobulin and albumin during the treatment period (p = 0.001).Conclusions: The addition of salmeterol to fluticasone produces no sustained effect on allergen induced cellular bronchial inflammation but leads to a significant improvement in size selectivity of plasma protein permeation across the respiratory membrane. This may contribute to the improved clinical outcome seen in patients with allergic asthma when a long acting b 2 agonist is combined with inhaled corticosteroids.

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Allergen‐induced bronchial inflammation in house dust mite‐allergic patients with or without asthma

Cited by 35 publications

References 30 publications

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

IL-4 activates equine neutrophils and induces a mixed inflammatory cytokine expression profile with enhanced neutrophil chemotactic mediator release ex vivo

Adding salmeterol to an inhaled corticosteroid: long term effects on bronchial inflammation in asthma

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