Allergen‐induced basophil activation: CD63 cell expression detected by flow cytometry in patients allergic to <i>Dermatophagoides pteronyssinus</i> and <i>Lolium perenne</i>

Sánz, M L; Sánchez, G.; Gamboa, P. M.; Vila, Leticia; Uasuf, Carina Gabriela; Chazot, M.; Diéguez, I; Weck, A.L. de

doi:10.1046/j.1365-2222.2001.01122.x

Cited by 100 publications

(108 citation statements)

References 19 publications

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“…Flow cytometric analysis of in vitro activated basophils using CD63 neo-expression has proved quite reliable for the diagnosis of different IgE-mediated allergies including allergies to inhalant allergens (e.g. pollen allergy) [10], food [2] and venom [3]. It has also been used with some success in evaluating reactions to drugs, including muscle relaxants.…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

et al. 2005

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SummaryThe investigation of anaphylactic reactions in the peri-operative period is difficult. Elevation of serum tryptase levels is a good indicator of an anaphylactic event but the ability of subsequent investigations to identify the drug(s) responsible for the reaction is still potentially unreliable. The aim of this study was to examine basophil activation as an investigative tool. We performed flow cytometric analysis of the expression on the cell surface of the basophil activation markers CD63 and CD203c and measured histamine release in 21 patients who were referred with possible peri-operative anaphylaxis. The sensitivity of CD63, CD203c, basophil histamine release and skin prick for the muscle relaxants was found to be 79%, 36%, 36% and 64%, respectively; the specificity was found to be 100%. These results demonstrate the difficulty in investigating the cause of an unexpected clinical event following drug administration, but the higher sensitivity of neo-expression on the cell surface of CD63 suggests that flow cytometric analysis of its neo-expression on basophils in vitro may be a diagnostic aid.

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Section: Discussionmentioning

confidence: 99%

Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

et al. 2005

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“…Specific IgE blood testing may be preferred by primary care physicians because testing is relatively ease to manage and results are highly accurate [24 -28]. Another test, the basophil activation test is also a highly reliable technique in the diagnosis of allergy to inhalant allergens [29]. Findings from other studies; however, posit that specific IgE blood levels may not be consistent if a patient is followed using 2 different assay systems [30].…”

Section: Discussionmentioning

confidence: 99%

Sleep Disorders and Chronic Constipation: Relation to Other Co-morbidities?

Szeinbach¹,

Rodríguez-Monguió²,

Baran³

et al. 2010

TOALLJ

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Sleep disorders are common complaints and frequently associated with a number of disease states. Although the link between sleep disorders, respiratory diseases, and other co-morbid conditions experienced by patients has been investigated, the link between sleep disorders and chronic constipation is relatively unexplored. Given the widespread occurrence of sleep disorders, it is important to evaluate how sleep disorders relate to respiratory diseases and other comorbid disease states in participants with chronic constipation. Thus, the purpose of this study was to identify co-morbid conditions that were significant predictors for chronic constipation participants with and without sleep disorders. Of the 311 participants with a confirmed diagnosis of chronic constipation, 84% of the sample was female with 52% having sleep disorders, approximately two-thirds reported allergies, and 30% or less reported chronic sinusitis, chronic bronchitis, asthma, and chronic obstructive pulmonary disease (COPD). Results from logistic regression analysis revealed significant odds ratios for sleep disorders and allergies (OR 2.4; p = 0.001), asthma (OR 2.0; p = 0.01), chronic bronchitis (OR 2.2; p = 0.014), COPD (OR 4.0; p < 0.001), sinusitis (OR 2.0; p = 0.008), and for other co-morbid conditions. Perceptions of overall health were significantly worse (p < 0.001) for chronic constipation participants with certain co-morbid conditions including sleep disorders, respiratory diseases, and disease states producing pain. Co-morbid conditions may influence information provided by patients when discussing family history, diagnosing, discussing pharmacotherapy, and monitoring patient-reported outcomes. Co-morbid conditions should be considered by physicians and specialists during patient assessment and evaluation.

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“…In vitro challenge of basophils with Gelofusine All participants underwent the BAT with Gelofusine, employing a technique using whole blood samples modified from that described by Sanz et al [12]. Heparinised blood (100 ll) was incubated with 20 ll stimulation buffer containing 2 ng.ml ; Sigma) was added to each tube and flow cytometric analysis performed using a FACScalibur flow cytometer and CELL QUEST software (BD Biosciences, San Jose, CA).…”

Section: Methodsmentioning

confidence: 99%

Anaphylaxis to Gelofusine^® confirmed by in vitro basophil activation test: a case series*

Apostolou¹,

Deckert²,

Puy³

et al. 2006

Anaesthesia

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Summary The plasma expander Gelofusine® (succinylated gelatin) is a recognised cause of peri‐operative anaphylaxis. Current diagnosis of Gelofusine sensitivity is by skin testing, a procedure that itself carries a risk of allergic reaction. We evaluated the reliability of the in vitro basophil activation test as a diagnostic assay for Gelofusine sensitivity in subjects with a clinical history highly suggestive of Gelofusine allergy. Six patients with peri‐operative anaphylaxis clinically attributed to Gelofusine were skin tested to confirm sensitivity. Control subjects included three healthy subjects and five subjects allergic to a neuromuscular blocking drug, all negative on Gelofusine skin testing. Whole blood basophil activation to Gelofusine was analysed by flow cytometry for CD63 surface expression. All of the Gelofusine sensitive patients and one of the control allergic subjects showed positive basophil activation to Gelofusine. In this series of subjects, the basophil activation test for Gelofusine allergy had a sensitivity of 100% and a specificity of 87.5%. Our findings suggest that basophil activation testing is a safe and reliable in vitro assay for prediction or confirmation of Gelofusine sensitivity in patients with high clinical suspicion of Gelofusine‐induced anaphylaxis.

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Allergen‐induced basophil activation: CD63 cell expression detected by flow cytometry in patients allergic to Dermatophagoides pteronyssinus and Lolium perenne

Abstract: The basophil activation test is a highly reliable technique in the diagnosis of allergy to inhalant allergens. The sensitivity of the basophil activation test was 93.3%, and its specificity 98.4%, when using a cut-off point of 15% activated basophils as positive result.

Cited by 100 publications

References 19 publications

Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

Sleep Disorders and Chronic Constipation: Relation to Other Co-morbidities?

Anaphylaxis to Gelofusine^® confirmed by in vitro basophil activation test: a case series*

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Allergen‐induced basophil activation: CD63 cell expression detected by flow cytometry in patients allergic to Dermatophagoides pteronyssinus and Lolium perenne

Abstract: The basophil activation test is a highly reliable technique in the diagnosis of allergy to inhalant allergens. The sensitivity of the basophil activation test was 93.3%, and its specificity 98.4%, when using a cut-off point of 15% activated basophils as positive result.

Cited by 100 publications

References 19 publications

Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

Flow cytometric investigation of peri‐anaesthetic anaphylaxis using CD63 and CD203c

Sleep Disorders and Chronic Constipation: Relation to Other Co-morbidities?

Anaphylaxis to Gelofusine® confirmed by in vitro basophil activation test: a case series*

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Product

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Anaphylaxis to Gelofusine^® confirmed by in vitro basophil activation test: a case series*