Allergen Homologues, Pathogenesis-Related 1, Polygalacturonase, and Pectin Methyl Esterase from a Japanese Hop

Jang, Seok Woo; Jeong, Kyoung Yong; Yuk, Ji Eun; Lee, Jae Hyun; Park, Kyung Hee

doi:10.2174/0929866527666200813201924

Cited by 8 publications

(6 citation statements)

References 31 publications

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“… 19 In our investigation, the observed IgE reactivity to Hum j 6 together with PME could have resulted in IgE reactivity to a 50 kDa component due to poor dissociation in the protein gel. In previous study, Japanese hop PME demonstrated only weak IgE reactivity 5 ; however associated with PMEI the observed IgE binding as well as inhibition with purified natural Hum j 6 could be explained.…”

Section: Discussionmentioning

confidence: 80%

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Characterization of Hum j 6, a Major Allergen From Humulus japonicus Pollen, the Primary Cause of Weed Pollinosis in East Asia

Jeong,

Sang,

Lee

et al. 2023

Allergy Asthma Immunol Res

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Purpose Humulus japonicus ( HJ ) is one of the most important causes of weed pollinosis in East Asia. The 10 kDa protein with pI 10 in 2-dimensional gel has been recognized as the representative major allergen of HJ , but its major allergens have not been characterized. This study aimed to characterize the major allergen of HJ . Methods A major allergen in Japanese hop was detected by proteome analysis; it was purified to homogeneity and its sequence was obtained by transcriptome analysis. The recombinant proteins were produced in Escherichia coli and Pichia expression systems, and their immunoglobulin E (IgE) reactivities were compared to those of the natural counterpart. We also analyzed post-translational modifications such as glycosylation and phosphorylation. Results Pectin methylesterase inhibitor, Hum j 6, was found to be the major allergen of HJ , and in silico signal peptide prediction corresponds to a 15.1 kDa protein with a theoretical pI of 8.28. Natural Hum j 6 was recognized by IgE antibodies from 86.4% (19/22) of HJ pollinosis patients, whereas the recombinant proteins did not show strong IgE reactivity. No glycosylation was detected, while at least 15 phosphorylated amino acids, possibly causing the pI and molecular weight shift, were detected by tandem mass spectrometry analysis. Conclusions Hum j 6 was identified as the representative major allergen of HJ and seems to be modified significantly after translation. These findings are useful for the development of component-resolved diagnosis and immunotherapy.

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Section: Discussionmentioning

confidence: 80%

“… 3 Several proteins belonging to allergenic families, such as pathogenesis-related 1 (PR-1) protein, pectin methylesterase (PME), polygalacturonase, and profilin, were identified in HJ pollen by expressed sequence tag analysis, but displayed low allergenicity. 4 5 …”

Section: Introductionmentioning

confidence: 99%

Characterization of Hum j 6, a Major Allergen From Humulus japonicus Pollen, the Primary Cause of Weed Pollinosis in East Asia

Jeong,

Sang,

Lee

et al. 2023

Allergy Asthma Immunol Res

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“…Moreover, screening a cDNA expression library revealed that a PR-1 protein from Japanese hop ( Humulus japonicus ), an important cause of weed pollinosis in East Asia, is recognized by IgE from 3.4% (1/29) of Japanese hop allergic patients with a positive case history and IgE-sensitization to Japanese hop ( 62 ). Hum j PR-1 encodes for a 171 aa peptide (apparent MW of 20 kDa) and shares 40–46% of aa-sequence identity with Art v 2, Cuc m 3, and Cyn d 24 ( Table 1 ).…”

Section: Pr-1 Allergensmentioning

confidence: 99%

Allergenic Properties and Molecular Characteristics of PR-1 Proteins

et al. 2022

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Only a small fraction of proteins in plants and animals are classified as allergens. The allergenic properties are frequently attributed to certain functional characteristics of the proteins, such as a role in the plant defense against biotic and abiotic stress, to achieve the systematic acquired resistance. In line with this, eight members out of 17 functional pathogenesis-related (PR) protein families have been characterized as allergens. The present review summarizes the molecular features and allergenic significance of allergens of the PR-1 family. Not many allergens have been identified as belonging to this protein family, with most of them having a pollen origin, like mugwort or Bermuda grass. Molecular and structural features of allergenic PR-1 proteins are discussed and attributed to their IgE-reactive properties, clinical manifestation, and cross-reactivity among different foods and inhalants.

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“…A 10 kDa component is known to be a major allergen ( 7 ) even though it has not been cloned. Recombinant proteins of profilin, pathogenesis-related 1, polygalacturonase, and pectin methyl esterase, homologous to pollen allergens from Japanese hop, were recognized with 3.4 to 13.8% of IgE antibodies from the patients ( 9 , 10 ).…”

Section: Inhalant Allergens Of Regional Importancementioning

confidence: 99%

Allergens of Regional Importance in Korea

Jeong

2021

Front.Allergy

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Allergen repertoire should reflect the region's climate, flora, and dining culture to allow for a better diagnosis. In Korea, tree pollens of oak and birch in the spring in conjunction with weed pollens of mugwort, ragweed, and Japanese hop are the main causes of seasonal allergic rhinitis. More specifically, the sawtooth oak in Korea and the Japanese hop in East Asia make a difference from western countries. Among food allergens, the sensitization to silkworm pupa and buckwheat is also common in Korean patients. Honey bee venom due to apitherapy in traditional medicine and Asian needle ant, Pachycondyla chinensis, are important causes of anaphylaxis in Korea. Climate change, frequent overseas traveling, and international product exchanges make situations more complicated. Ragweed, for example, was not native to Korea, but invaded the country in the early 1950s. Recently, Japanese hop and Asian needle ants have been recognized as important invasive ecosystem disturbing species in western countries. However, the molecular properties of the component allergens from these unique culprit allergens have been poorly characterized. The present review summarizes the molecular studies on the allergens of regional importance in Korea.

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Allergen Homologues, Pathogenesis-Related 1, Polygalacturonase, and Pectin Methyl Esterase from a Japanese Hop

Cited by 8 publications

References 31 publications

Characterization of Hum j 6, a Major Allergen From Humulus japonicus Pollen, the Primary Cause of Weed Pollinosis in East Asia

Characterization of Hum j 6, a Major Allergen From Humulus japonicus Pollen, the Primary Cause of Weed Pollinosis in East Asia

Allergenic Properties and Molecular Characteristics of PR-1 Proteins

Allergens of Regional Importance in Korea

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