Allergen cross‐reactivity between Pityrosporum orbiculare and Candida albicans

Abstract

Pityrosporum orbiculare and Candida albicans extracts were separated by SDS-PAGE, and IgE binding was detected by immunoblotting with 21 patient sera that were RAST positive to both yeasts. Cross-wise inhibition was performed of IgE binding of a serum pool containing IgE antibodies to both yeasts. The pool was mixed with serial dilutions of P. orbiculare or C. albicans extracts, and incubated with strips containing separated allergen. IgE binding was quantified by densitometric scanning and percent inhibition was calculated as well as the respective ratios between required extract concentration for 50% inhibition in heterologous compared to homologous inhibition for each component (inhibition ratio). Ten components of P. orbiculare were detected by more than 60% of the sera. IgE binding to C. albicans was weak, and only to four bands was IgE binding detected by more than 30% of the sera. The most important C. albicans allergen was a 48-kDa band, to which IgE of half of the patient sera bound. There was little inhibition of IgE binding to P. orbiculare with C. albicans. Thus, all but three components exhibited an inhibition ratio higher than 100. The inhibition ratio of the 48-kDa C. albicans compound was 50, thus indicating some degree of cross-reactivity. Significant cross-reactivity was shown by C. albicans compounds of 18, 24, 26, 34, and 38 kDa, the inhibition ratios of which were less than 10. There was some degree of cross-reactivity between apparent protein allergens of the two yeasts, but IgE antibodies to C. albicans do not merely reflect sensitization to P. orbiculare.

Autoantibodies to estrogen receptor α interfere with T lymphocyte homeostasis and are associated with disease activity in systemic lupus erythematosus

“…To evaluate the antibody specificity, the serum pool obtained from patients with SLE who were positive for anti-ER␣ antibodies was diluted 1:100 in PBST and incubated overnight at room temperature in the presence of 40 g/ml of ER␣ (Sigma), according to the method described by Huang and colleagues (27). As a negative control, the serum pool was preincubated with 40 g/ml of bovine serum albumin (BSA; Sigma).…”

Cutaneous yeast microflora in patients with atopic dermatitis

“…Apart from sensitizations to species-specific antigens, a number of polyvalent sensitizations have been found that probably are caused by cross-reactivity between homologous proteins and the polysaccharides common among different fungal species. Cross-reactivity between Malassezia and Candida albicans, molds, and even non-fungal species has been shown [18][19][20][21]. These data can explain why fungi-allergic patients show reactivity to several fungal species [22].…”

Pathogenetische Bedeutung der intestinalen Candidabesiedelung

“…Urticaria nicht bestätigen konnten oder eine Kreuzreaktivität zwischen Candida albicans und Pityrosporum ovale bzw. orbiculare sowie Saccharomyces cerevisiae zeigten [183,184,185,186]. Leider wurde in einer Studie von Middleton et al [134] …”