Allelopathy and Identification of Volatile Components from the Roots and Aerial Parts of Astragalus mongholicus Bunge

Abstract

The volatile compounds produced by plants play an important role in plant growth, plant communication, and resistance to biological and abiotic stresses. Astragalus membranaceus var. mongholicus (AM) is a perennial herbaceous plant (Leguminosae) that is widely cultivated in northwest China. The bioactive compounds in its root have shown various pharmacological activities. Root rot disease caused by Fusarium spp. often occurs in AM planting with increasing severity in continuous monoculture. It is currently still unclear what are the effects of the volatile compounds produced by fresh AM on itself, other crops cultivated on the same field after AM, pathogen, and rhizobia. In this study, we found that seed germination and seedling growth of AM, lettuce (Lactuca sativa L.), and wheat (Triticum aestivum L.) could be affected if they were in an enclosed space with fresh AM tissue. Additionally, 90 volatile compounds were identified by SPME-GC-MS from whole AM plant during the vegetative growth, 36 of which were specific to aerial parts of AM (stems and leaves, AMA), 17 to roots (AMR), and 37 were found in both AMA and AMR. To further identify the allelopathic effects of these volatile compounds, five compounds (1-hexanol, (E)-2-hexenal, (E,E)-2,4-decadienal, hexanal, and eugenol) with relatively high content in AM were tested on three receptor plants and two microorganisms. We found that (E,E)-2,4-decadienal and (E)-2-hexenal showed significant inhibitory effects on the growth of AM and lettuce. One-hexanol and hexanal suppressed the growth of wheat, while eugenol showed a similar effect on all three plant species. Moreover, the activities of these compounds were dose dependent. Notably, we discovered that (E)-2-hexenal and eugenol also inhibited the growth of the pathogen Fusarium solani by as high as 100%. Meanwhile, all five compounds tested suppressed the rhizobia Sinorhizobium fredii. In summary, this study furthered our understanding of the comprehensive allelopathic effects of the main volatile components of AM.