Allelic Variation at the VRN-1 Locus of Polish Cultivars of Common Wheat (Triticum aestivum L.)

Nowak, Michał; Kowalczyk, Krzysztof

doi:10.2478/v10182-010-0028-2

Cited by 4 publications

(2 citation statements)

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“…Analysis of amplified PCR products confirmed 100 % identity in DNA sequences between common wheat and triticale. Nowak and Kowalczyk (2010) confirmed that such mechanism is a main growth habit regulator of Polish common wheat accessions). Results presented in this paper extended this finding to triticale as well.…”

Section: Discussionsupporting

confidence: 53%

Analysis of VRN1 gene in triticale and common wheat genetic background

Nowak

Leśniowska-Nowak

Zapalska

et al. 2014

Sci. agric. (Piracicaba, Braz.)

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In cereals, the transition from the vegetative stage to flowering is controlled in the main by the set of vernalization genes. Within these genes the most important role is played by VRN1, which encodes a MADS-box transcription factor, regulating the transition of shoot apical meristem to the reproductive phase. The level of vernalization requirement is strongly linked to the molecular structure of this gene. In this study we analyzed molecular mechanisms regulating the vernalization requirement in triticale on the basis of comparative analysis of the VRN1 locus between triticale (×Triticosecale Witt.) and common wheat (Triticum aestivum L.) genotypes. We also estimated the influence of VRN genotype on heading time and the winter hardiness of these two species. Molecular markers developed for VRN genotype detection in common wheat were successfully applied to an analysis of triticale genomic DNA. Subsequent analysis of the amplicons nucleotide sequence confirmed full similarity of the products obtained between triticale and common wheat. All winter triticale cultivars tested contained the recessive vrn-A1 allele, whereas all spring genotypes carried the dominant Vrn-A1a allele. Molecular analysis of the Vrn-B1 gene revealed the presence of the dominant Vrn-B1b allele in only one of the triticale genotypes analyzed (Legalo). The major system of determination of the vernalization requirement in triticale was transferred from common wheat without changes and is based on an alteration in the VRN1 gene promoter sequence within the A genome.

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Section: Discussionsupporting

confidence: 53%

Analysis of VRN1 gene in triticale and common wheat genetic background

Nowak

Leśniowska-Nowak

Zapalska

et al. 2014

Sci. agric. (Piracicaba, Braz.)

Self Cite

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show abstract

“…Presence of insertions or deletions in regulatory regions (promoter, first intron) of the three homoeologous genes found in hexaploid wheat (Vrn-A1, Vrn-B1, Vrn-D1), are associated with dominant alleles for spring growth habit (Yan et al, 2004a;Fu et al, 2005;Shcherban et al, 2012). Different combinations of Vrn-A1, Vrn-B1, and Vrn-D1 spring (dominant) alleles are the most common sources of spring growth habit among landraces and commercial cultivars of polyploid wheat around the world (Fu et al, 2005;Iqbal et al, 2007Iqbal et al, , 2010Nowak and Kowalczyk, 2010;Yan et al, 2004a;Zhang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%