Allelic shift in cis-elements of the transcription factor
            <i>RAP2.12</i>
            underlies adaptation associated with humidity in
            <i>Arabidopsis thaliana</i>

Lou, Shangling; Guo, Xiang; Liu, Lian; Song, Yan; Zhang, Lei; Jiang, Yuanzhong; Zhang, Lushui; Sun, Pengchuan; Liu, Bao; Tong, Shaofei; Chen, Ningning; Li, Meng; Zhang, Han; Liang, Ruyun; Feng, Xiaoqin; Zheng, Yudan; Liu, Huanhuan; Holdsworth, Michael J.; Liu, Jianquan

doi:10.1126/sciadv.abn8281

Cited by 21 publications

(24 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, there was no necessary connection between evolutionary relationship and presence/absence variation. Recently, it has been found that allelic changes of cis-regulatory elements of RAP2.12 are responsible for differentially regulating tolerance to drought and flooding in Arabidopsis (Lou et al, 2022). Therefore, in addition to presence/absence variation, variation in cis-acting elements should be taken into consideration when characterizing certain CaM/CML genes.…”

Section: Hvcams/cmls In Different Barley Genotypesmentioning

confidence: 99%

Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses

et al. 2022

View full text Add to dashboard Cite

Calmodulin (CaM) and calmodulin-like (CML) proteins are Ca2+ relays and play diverse and multiple roles in plant growth, development and stress responses. However, CaM/CML gene family has not been identified in barley (Hordeum vulgare). In the present study, 5 HvCaMs and 80 HvCMLs were identified through a genome-wide analysis. All HvCaM proteins possessed 4 EF-hand motifs, whereas HvCMLs contained 1 to 4 EF-hand motifs. HvCaM2, HvCaM3 and HvCaM5 coded the same polypeptide although they differed in nucleotide sequence, which was identical to the polypeptides coded by OsCaM1-1, OsCaM1-2 and OsCaM1-3. HvCaMs/CMLs were unevenly distributed over barley 7 chromosomes, and could be phylogenetically classified into 8 groups. HvCaMs/CMLs differed in gene structure, cis-acting elements and tissue expression patterns. Segmental and tandem duplication were observed among HvCaMs/CMLs during evolution. HvCML16, HvCML18, HvCML50 and HvCML78 were dispensable genes and the others were core genes in barley pan-genome. In addition, 14 HvCaM/CML genes were selected to examine their responses to salt, osmotic and low potassium stresses by qRT-PCR, and their expression were stress-and time-dependent. These results facilitate our understanding and further functional identification of HvCaMs/CMLs.

show abstract

Section: Hvcams/cmls In Different Barley Genotypesmentioning

confidence: 99%

Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses

et al. 2022

View full text Add to dashboard Cite

show abstract

“…This result contrasted with previous studies on woody plants and lowland regions (Wang et al., 2022 ), but was similar to Saxifraga (Liu et al., 2021 ). A recent physiological study also found that populations of the herbaceous plant Arabidopsis thaliana in Tibet suffered from higher drought stress compared to lowland populations in Sichuan, China (Lou et al., 2022 ). The narrow‐ranging species of Gentiana , which are mainly distributed in alpine mountain regions, might also suffer from higher drought stress than wide‐ranging species, thus showing a positive correlation with PWQ.…”

Section: Discussionmentioning

confidence: 98%

Global richness patterns of alpine genus Gentiana depend on multiple factors

Wai,

Liang,

Xie

et al. 2024

Ecology and Evolution

Self Cite

View full text Add to dashboard Cite

Environmental factors impact species richness differently across taxonomic groups, and understanding the geographic patterns and drivers influencing alpine plant richness remains limited. This study compiled global distribution data of 404 species of Gentiana, an alpine genus, and analyzed the relative effects of different environmental factors and several previously proposed models on the variation of Gentiana richness. By evaluating the effects of range size and regions on the relationships between Gentiana richness and environmental factors, we found that all tested environmental factors had weak effects on richness variation for all species and wide‐ranging species, while habitat heterogeneity was the best predictor for narrow‐ranging species. Habitat heterogeneity was the main driver of richness variation in Europe and Asia, but not in North America. The multiple regression model that included variables for energy, water, seasonality, habitat heterogeneity and past climate change had the highest explanatory power, but it still explained less than 50% of the variation in species richness for all Gentiana species at both global and regional scale, except for Europe. The limited explanatory power of environmental factors in explaining species richness patterns for all species, along with the variations observed among regions, suggest that other factors, such as evolutionary processes and biogeographic history may have also influenced the geographic patterns of Gentiana species richness. In conclusion, our results indicate a limited influence of climate factors on alpine species richness, while habitat heterogeneity, along with its impacts on speciation and dispersal, likely play significant roles in shaping the richness of alpine Gentiana species.

show abstract

“…The increase in leaf angle and petiole elongation under submergence has been reported previously for flood-adapted species with aerenchyma-rich tissues, such as species from the genus Rumex and Rorippa ( Cox et al 2003 ; Pierik et al 2005 ; Sasidharan and Voesenek 2015 ) as well as in Arabidopsis , which lacks aerenchyma ( van Veen et al 2016 ). However, unlike most previous submergence research on Arabidopsis ( Vashisht et al 2011 ; Gonzali et al 2015 ; van Veen et al 2016 ; Yuan et al 2017 ; Yeung et al 2018 ; Bui et al 2020 ; Lou et al 2022 ), our submergence treatments were performed in the light and the decline in internal oxygen concentrations might have been more gradual, as light availability during submergence allows for some underwater photosynthesis and supports oxygen production and carbon assimilation, making the stress milder than in the absence of light ( Vashisht et al 2011 ). This may also explain why we do not see a strong evidence of a physiological drought during the post-submergence phase in our experiments, in contrast to what has been reported previously ( Fukao et al 2011 ; Sasidharan and Voesenek 2015 ; Tamang and Fukao 2015 ; Yeung et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of sublethal single, simultaneous and sequential abiotic stresses on phenotypic traits of Arabidopsis thaliana

et al. 2022

View full text Add to dashboard Cite

Plant responses to abiotic stresses are complex and dynamic, and involve changes in different traits, either as the direct consequence of the stress, or as an active acclimatory response. Abiotic stresses frequently occur simultaneously or in succession, rather than in isolation. Despite this, most studies have focused on a single stress and single or few plant traits. To address this gap, our study comprehensively and categorically quantified the individual and combined effects of three major abiotic stresses associated with climate change (flooding, progressive drought and high temperature) on 12 phenotypic traits related to morphology, development, growth and fitness, at different developmental stages in four Arabidopsis thaliana accessions. Combined sublethal stresses were applied either simultaneously (high temperature and drought) or sequentially (flooding followed by drought). In total, we analysed the phenotypic responses of 1782 individuals across these stresses and different developmental stages. Overall, abiotic stresses and their combinations resulted in distinct patterns of effects across the traits analysed, with both quantitative and qualitative differences across accessions. Stress combinations had additive effects on some traits, whereas clear positive and negative interactions were observed for other traits: 9 out of 12 traits for high temperature and drought, 6 out of 12 traits for post-submergence and drought showed significant interactions. In many cases where the stresses interacted, the strength of interactions varied across accessions. Hence, our results indicated a general pattern of response in most phenotypic traits to the different stresses and stress combinations, but it also indicated a natural genetic variation in the strength of these responses. This includes novel results regarding the lack of a response to drought after submergence and a decoupling between leaf number and flowering time after submergence. Overall, our study provides a rich characterization of trait responses of Arabidopsis plants to sublethal abiotic stresses at the phenotypic level and can serve as starting point for further in-depth physiological research and plant modelling efforts.

show abstract

Allelic shift in cis-elements of the transcription factor RAP2.12 underlies adaptation associated with humidity in Arabidopsis thaliana

Cited by 21 publications

References 51 publications

Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses

Calmodulin and calmodulin-like gene family in barley: Identification, characterization and expression analyses

Global richness patterns of alpine genus Gentiana depend on multiple factors

Effects of sublethal single, simultaneous and sequential abiotic stresses on phenotypic traits of Arabidopsis thaliana

Contact Info

Product

Resources

About