Allelic loss of chromosome 13q14.3 in human oral cancer: Correlation with lymph node metastasis

Ogawara, Katsunori; Miyakawa, Akihisa; Shiiba, Masashi; Uzawa, Katsuhiro; Watanabe, Toshihide; Wang, Xiaoli; Sato, Takahiro; Kubosawa, Hitoshi; Kondo, Youichiro; Tanzawa, Hideki

doi:10.1002/(sici)1097-0215(19980821)79:4<312::aid-ijc2>3.0.co;2-y

Cited by 62 publications

(40 citation statements)

References 15 publications

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“…17,18,19,33 There are several reports that LOH is associated with more advanced stage and more aggressive HNSCC tumors, 13,34,36,37,56 and specifically with nodal involvement. 32,44 Other authors describe very specific correlations such as a link between LOH at 17p and mitotic index, 39 a connection of LOH at MLH1 with lower grade HNSCC, and LOH at CDKN2A with higher grade, 9 or a preference of LOH in tumors originating from the pharynx. 56 A correlation between LOH and other patient-related factors like age and race, was described in some studies.…”

Section: Lohmentioning

confidence: 99%

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The clinical relevance of microsatellite alterations in head and neck squamous cell carcinoma: a critical review

Schutter¹,

Spaepen²,

Bride

et al. 2007

Eur J Hum Genet

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Triggered by the existing confusion in the field, the current paper aimed to review the current knowledge of both microsatellite instability (MSI) and loss of heterozygosity (LOH) detected by microsatellite markers in head and neck squamous cell carcinoma (HNSCC), and to provide the reader with an assessment of their prognostic and predictive value in this tumor type. For both MSI and LOH, various detection methods were included such as mono-and polynucleotidemarkers and gel-as well as automated analyses. Only studies based on PCR techniques with microsatellite markers were considered. Taking the methodological problems occurring in investigations with microsatellite markers into account, LOH seems to be more common than MSI in HNSCC. Although both types of microsatellite alterations have been correlated with clinicopathological features of this tumor type, only LOH seems to have a clear prognostic value. The predictive value of both MSI and LOH is debatable. More research has to be performed to clearly establish LOH detection as a translational application in the HNSCC field, aiming to predict response to treatments or outcome, and eventually to use as a therapeutic target.

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Section: Lohmentioning

confidence: 99%

“…44,55 Furthermore, several studies have identified a negative prognostic role for LOH, 18,37,41,73,74 even in multivariate analyses. 34,35,46 This negative prognostic value is mostly connected to allelic loss at 3p, 8p, 11q, 13q, 17p and 18q.…”

Section: Prognostic Valuementioning

confidence: 99%

The clinical relevance of microsatellite alterations in head and neck squamous cell carcinoma: a critical review

Schutter¹,

Spaepen²,

Bride

et al. 2007

Eur J Hum Genet

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“…The following microsatellite markers purchased from Research Genetics (Huntsville, AL, USA) were selected based on previous reports (Uzawa et al, 1994(Uzawa et al, , 1996Gonzalez et al, 1995;Watanabe et al, 1997;Miyakawa et al, 1998;Ogawara et al, 1998;Nakanishi et al, 1999): D5S178 (5q21), D9S104 (9p21), IFNA (9p22), D11S910 (11q23), D11S1356 (11q25), D13S273 (13q14 -21), TP53 (17p13), D18S46 (18q21), and D22S274 (22q13). Polymerase chain reaction (PCR) amplification was carried out in a 25-ml final volume containing 0.6 U of Taq polymerase in PCR buffer (50 mM KCl, 10 mM Tris-Cl (pH 8.0), 1.5 mM MgCl 2 ), 80 mM of each dNTP, and 10 pmol of each primer.…”

Section: Microsatellite Analysismentioning

confidence: 99%

Monitoring of circulating tumour-associated DNA as a prognostic tool for oral squamous cell carcinoma

et al. 2005

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Frequent allelic imbalances (AIs) including loss of heterozygosity and microsatellite instability on a specific chromosomal region have been identified in a variety of human malignancies. The objective of our study was to assess the possibility of prognostication and monitoring of oral squamous cell carcinoma (SCC) by microsatellite blood assay. DNA from normal and tumorous tissues and serum DNA obtained at three time points (preoperatively, postoperatively, and 4 weeks postoperatively) from 64 patients with oral SCC was examined at nine microsatellite loci. In all, 38 (59%) DNA samples from tumorous tissues and 52% from serum showed AIs in at least one locus. Patterns of AIs in the serum DNA were matched to those detected in tumour DNA. Of them, AIs were frequently detected preoperatively (44%, 28 of 64), and postoperatively (20%, 13 of 64). Moreover, among 12 cases with AIs during the postoperative period, six had no evidence of an AI 4 weeks postoperatively, and they had no recurrence and were disease free. In contrast, six patients with AI-positive DNA 4 weeks postoperatively have died with distant metastasis within 44 weeks. Thus, our results suggest that the assessment of microsatellite status in the serum DNA could be a useful predictive tool to monitor disease prognosis.

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“…To evaluate LOH, we used 24 polymorphic microsatellite markers located at the following sites in the 10 genes reported to play a major role in human carcinogenesis: [13][14][15][16][17][18][19][20][21][22] FHIT (3p) (D3S1300, D3S1312, and D3S1313), APC (5q) (D5S346 and D5S82), p16 (9p) (D9S171 and D9S162), TSC-1 (9q) (D9S149, D9S150 and DBH), Int-2 (11q) (INT-2), Rb (13q) (D13S270, D13S273, and D13S176), TSC-2 (16p) (D16S291 and D16S292), p53 (17p) (TP53 and D17S520), Smad 4 (18q) (D18S46, D18S363, and D18S474), and Band M (22q) (D22S1140, D22S1170, and D22S1161). The use of more than one microsatellite marker ensured a higher yield of information for each genomic locus.…”

Section: Multiplex Polymerase Chain Reaction-loss Of Heterozygosity Amentioning

confidence: 99%

Topographical distribution of allelic loss in individual lung adenocarcinomas with lymph node metastases

et al. 2004

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Adenocarcinomas of the lung are characterized by morphological heterogeneity, and since carcinogenesis has been suggested to be a multistep process involving sequential accumulation of multiple genetic alterations, the morphological heterogeneity may represent a cross-sectional view of genetic alterations within individual tumors. Therefore, to elucidate whether, and which, genetic alterations accumulated in relation to morphological cancer progression, we examined 56 microdissected sites for topographical distribution of loss of heterozygosity (LOH) in 12 adenocarcinomas of the lung with bronchioloalveolar (BA) and invasive components in their primary tumors and metastases to lymph nodes. The morphological changes from noninvasive BA lesions to invasive and metastatic components were characterized by a significant rise in the prevalence of allelic losses (Po0.05). Individually, eight cases (67%) showed accumulation of genetic alterations from BA lesions to metastases. LOHs in multiple foci in one case were compared to determine whether they were shared at all tumor sites as an early event or localized in metastases as an additional event.LOHs at 5q and 17p may be crucial steps in the early phase of development to metastasis, while 18q loss may be an additional step. These findings suggested that the cancer cells in some pulmonary adenocarcinomas evolved from the BA lesions to the invasive and metastatic lesions.

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Allelic loss of chromosome 13q14.3 in human oral cancer: Correlation with lymph node metastasis

Cited by 62 publications

References 15 publications

The clinical relevance of microsatellite alterations in head and neck squamous cell carcinoma: a critical review

The clinical relevance of microsatellite alterations in head and neck squamous cell carcinoma: a critical review

Monitoring of circulating tumour-associated DNA as a prognostic tool for oral squamous cell carcinoma

Topographical distribution of allelic loss in individual lung adenocarcinomas with lymph node metastases

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