Allelic diversity in Bulgarian winter wheat varieties based on polymorphism of glutenin subunit composition

Tsenov, N.; Atanasova, D.; Todorov, Ivan; Ivanova, I.; Stoeva, I.

doi:10.1556/crc.37.2009.4.8

Cited by 14 publications

(8 citation statements)

References 19 publications

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“…Storage protein conformation is thought as constant element of cultivar due to direct genome expression therefore; it can help in identification of cultivar. Our results are in accordance with the earlier studies of (Popa et al 2003;Kang et al 2007;Tsenov et al 2009).…”

Section: Discussionsupporting

confidence: 94%

Characterization of Pakistani wheat germplasm for high and low molecular weight glutenin subunits using SDS-PAGE

Khalid

Hameed

2019

Cereal Research Communications

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High molecular weight (HMW-GS) and low molecular weight (LMW-GS) glutenin subunits play a significant role in bread making quality and extensibility, though they signify merely 10% and 40% of the entire seed storage proteins. For the estimation of bread quality on the basis of allelic difference in HMW-GS and LMW-GS at Glu-1 and 3 loci, wheat germplasm (77 genotypes) was collected from diverse agro-climatic regions of Pakistan and characterized by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Thirty distinct allelic arrangements were identified with a sum of thirteen Glu-1 alleles. Maximum frequency of allele 1 was found in twenty-nine genotypes at Glu-A1 locus while high proportion of subunit pairs 13 + 16 and 2 + 12 was detected in 33 and 32 genotypes at Glu-B1 as well as Glu-D1 locus, respectively. Few rare alleles were also separated out. The quality scores ranged from 4-10, however highest quality score of ten was more recurrent (36.36%). A good quality score of 8 and 6 were found in 32.47% as well as 19.48% of genotypes individually. In LMW-GS, seventeen diverse combinations of alleles with aggregate of ten Glu-3 alleles were detected. Glu-A3c and Glu-B3d alleles were observed in 33 (42.85%) genotypes, encoding high sedimentation and protein contents. Hence, this will enable the breeders to utilize both glutenin subunits as biochemical indicator for selecting superior wheat genotypes possessing enhanced bread making quality.

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Section: Discussionsupporting

confidence: 94%

Characterization of Pakistani wheat germplasm for high and low molecular weight glutenin subunits using SDS-PAGE

Khalid

Hameed

2019

Cereal Research Communications

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“…Molecular weight and quantification of individual HMW-GS by the LoaC method of nine Croatian and Serbian bread wheat cultivars are represented in Tables 2 and 3, respectively. The most frequent HMW-GS combination in both assortments are 2 * , 7 + 9, and 5 + 10 ("Divana, " "Zlata, " "Pobeda, " "Bastijana, " and "NS3-5299") for which the authors [18][19][20] suppose that, together with N, 7 + 9, and 5 + 10, HMW-GS combination dominates in European winter wheat cultivars. Beside this combination, HMW-GS combinations 7 + 9 and 2 + 12 ("Dragana, " "Simonida, " and "Zvezdana"); 2 * , 7 + 8, and 5 + 10 ("Soissons" and "Felix"), and N, 7 + 9, and 5 + 10 ("Ljiljana" and "Etida") are also frequent in this study.…”

Section: Resultsmentioning

confidence: 99%

Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation

Živančev

Horvat

Torbica

et al. 2015

Journal of Chemistry

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RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method. Strong correlation ( ≤ 0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods. Significant correlations ( ≤ 0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area. Even more significant correlation ( ≤ 0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW. RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.

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“…Maintenance of Glu-A1b (2*) and Glu-D1d (5+10) alleles is important. These alleles contribute to quality supported with Glu-B1i (17+18), Glu-B1f (13+16) and Glu-B1h (14+15) (Tsenov et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Allele Frequency of Glutenin Subunits and Glu-1 Quality Scores in Some Turkish Bread Wheat Landraces

Temizgül¹,

Akbulut²

2020

Trakya University Journal of Natural Sciences

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There are eight centers of origin for cultivated plants and Turkey is located in the interception of two of these centers, the Near East and the Mediterranean. Therefore, Turkey is known to be the gene center for diversification and dispersion of such main cereal crops such as wheat, barley, rye and oat. This study was performed to determine glutenin gene allele frequencies and Glu-1 quality scores of 116 local wheat landraces of Turkish bread wheat. SDS-PAGE and PCR were used to identify glutenin gene alleles. The results showed that the studied Turkish local wheat landraces contained a total of 19 different subunits (3 subunits in Glu-A1, 11 in Glu-B1 and 5 in Glu-D1) with 50 different combinations. The highest and the lowest allelic combinations were determined in East Anatolia and the Aegean regions, respectively. Glu-A1c (65.11%), Glu-B1b (53.60%) and Glu-D1a (58.30%) were the most frequent alleles. The Glu-1 quality score was found to be 6.07 for the studied genotypes. Among the regions, the highest (7.18) and the lowest (4.80) mean Glu-1 scores were detected in Marmara and Southeastern Anatolia regions, respectively. 4 accessions (TR32846-6, TR36948-1, TR45105 and TR63536) were reported to have the highest Glu-1 quality score as 10. 6 genotypes (TR45398-4, TR48025-3, TR33264-6, TR393-5, TR52021-3 and TR45094) had the quality score of 9. Including more new landraces may contribute to discover new Glu-1 alleles. Özet: Kültür bitkileri için sekiz orijin merkezi vardır ve Türkiye, bu merkezlerden ikisinin, Yakın Doğu ve Akdeniz'in, kesiştiği yerdedir. Türkiye bu nedenle buğday, arpa, çavdar ve yulaf gibi ana tahıl bitkilerinin çeşitlendiği ve dağıldığı gen merkezi olarak bilinir. Bu çalışmanın amacı, Türk ekmeklik buğdaylarından 116 yerel buğday ırkının glüten allel sıklığı ve Glu-1 kalite skorunu belirlemektir. SDS-PAGE ve PCR, glüten allellerini tanımlamak için kullanılmıştır. İstatistiksel analizler için POPGENE 1.31 yazılımı kullanılmıştır. İncelenen Türk yerel buğday ırkları toplam 19 farklı alt birim (Glu-A1'de 3 alt birim, Glu-B1'de 11 ve Glu-D1'de 5 alt birim) ve 50 farklı kombinasyon içermektedir. En yüksek ve en düşük allel kombinasyonları sırasıyla Doğu Anadolu ve Ege bölgelerinde belirlenmiştir. Glu-A1c (% 65,11), Glu-B1b (% 53,60) ve Glu-D1a (% 58,30) en sık görülen alleller olarak tespit edilmiştir. Glu-1 kalite skoru, Türkiye genelinde incelenen genotipler için 6,07 olarak bulunmuştur. Bölgeler arasında ortalama en yüksek (7,18) ve en düşük (4,80) Glu-1 skorları sırasıyla Marmara ve Güneydoğu Anadolu bölgelerinde tespit edilmiştir. Çalışılan 116 aksesyondan 4 tanesi (TR32846-6, TR36948-1, TR45105 ve TR63536) kalite skoru 10 olup en yüksek Glu-1 kalite skoruna sahipken 6 tanesinin ise (TR45398-4, TR48025-3, TR33264-6, TR393-5, TR52021-3 ve TR45094) kalite skoru 9 olarak hesaplanmıştır. Daha fazla yerel çeşitlerin çalışmalara eklenmesi yeni Glu-1 allelerinin keşfedilmesine katkıda bulunabilir.

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Allelic diversity in Bulgarian winter wheat varieties based on polymorphism of glutenin subunit composition

Cited by 14 publications

References 19 publications

Characterization of Pakistani wheat germplasm for high and low molecular weight glutenin subunits using SDS-PAGE

Characterization of Pakistani wheat germplasm for high and low molecular weight glutenin subunits using SDS-PAGE

Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation

Allele Frequency of Glutenin Subunits and Glu-1 Quality Scores in Some Turkish Bread Wheat Landraces

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