Allele frequencies of fifteen STR loci in a population from Central Brazil

Silva, Myrnzzia Beatriz; Silva, Daniela de Melo e; Rodovalho, Ricardo Goulart; Rios, Paulene Alves; Silva, Weliçon Menezes; Cruz, Aparecido Divino da

doi:10.1016/j.fsigen.2009.10.008

Cited by 6 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the author recommended the use of high fidelity DNA polymerases in PCR multiplex as in genotyping of microsatellites to major accuracy and fidelity in the results, in which was proved in this work. Silva et al (2010) also used PCR multiplex including combinations of DNA polymerases (High difelity) to amplification of fifteen microsatellites. In this study we recommended to use of high fidelity enzymes of DNA polymerases to suitable e and accuracy to amplify of PCR multiplex, manley to use to microsatellites.…”

Section: Pcr and Microsatellite Analysismentioning

confidence: 99%

Avaliação de DNA polimerases em ensaios de amplificação de microssatélites através do PowerPlex®16 BIO System

Rocha¹,

Miranda

maranguape

2014

BBR - Biochem. Biotechnol.

View full text Add to dashboard Cite

This study aimed to compare several DNA polymerases in order to optimize costs of microsatellite amplification reaction through the PowerPlex®16 BIO System. Three different polymerase brands have been used: (i) Paq5000 DNA Polymerase (Stratagene) and (ii) Platinum® Taq DNA Polymerase (Invitrogen), which have low commercial price; and (iii) Advantage 2 Polymerase Mix (Clontech), a DNA polymerase with high commercial price. Herein, neither samples nor positive control amplified in the reactions carried out with Paq5000 DNA Polymerase. Furthermore, the reactions performed with Platinum® Taq DNA Polymerase showed contrasting results, amplifying alleles only in some samples. On the other hand, the two microsatellite loci used (amelogenin e D18S51) were amplified in the analyses carried out with the Advantage 2 Polymerase Mix. Our findings indicated that the enzymes Paq5000 DNA Polymerase and Platinum® Taq DNA Polymerase have low processing capacity, showing inefficiency in the microsatellite detection; and that Advantage 2 Polymerase Mix is highly effective and suitable for microsatellite amplification assays.

show abstract

Section: Pcr and Microsatellite Analysismentioning

confidence: 99%

Avaliação de DNA polimerases em ensaios de amplificação de microssatélites através do PowerPlex®16 BIO System

Rocha¹,

Miranda

maranguape

2014

BBR - Biochem. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…For this reason, the Brazilian population is ideal for studies of allele frequencies, as there exists a high degree of miscegenation in this population, with ethnicities from diverse regions of the world (Rodrigues et al, 2009;Silva et al, 2009;Vieira et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goiás, Central Brazil

Rodovalho¹,

Santos²,

Cavalcanti³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. In studies of human identification, obtaining a high standard of outcomes and satisfactory conclusions are directly related to the use of highly polymorphic molecular markers. In addition to the combined DNA index system (CODIS) group, it is also important to implement non-CODIS markers into the analysis, as they increase the power of discrimination. During the identification process, it is essential to consider the genetic variation among distinct groups of populations, as the allele frequencies 7311 CODIS and non-CODIS STR allele frequencies in Brazil ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7310-7314 (2015) are directly associated with the power of discrimination. However, the population of Goiás, a State located in Central Brazil, is characterized by a highly mixed population due to its diverse ethnic origins. In this study, a survey of the allelic frequencies in the Goiás population was carried out using a molecular assembly composed of 21 autosomal loci both from and external to the CODIS group. The new data, for some of the markers used, were statistically similar to those from previous studies. This consistency means that the use of these markers might serve as a parameter for future population comparisons. The results from these analyses further our knowledge of the study of human identification.

show abstract

“…Moreover, the study of human race has been of interest since ancient times. The existence of differences between populations of distinct geographical origins must have been a finding of the first long-distance travelers (Silva et al, 2010). Nowadays, considerable research has been conducted to identify the geographical origin of people and their closeness, from several points of view, as well as the degree of admixture in the genetic composition of a population.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Y-STR haplotype diversity and population data for Central Brazil: implications for environmental forensics and paternity testing

et al. 2014

View full text Add to dashboard Cite

ABSTRACT. The central region of Brazil was colonized by internal migration of individuals of different origins, who contributed to the genetic diversity existing in this population. This study determined the allele frequencies and haplotype diversity of Y-STRs in Goiás State, Central Brazil, and compared the data obtained with a sample of the Brazilian population, consisting of individuals from the five geographical regions of Brazil. A total of 353 males were typed for Y-STR haplotype diversity in Central Brazil 12 Y-chromosome short tandem repeat (Y-STR) markers. We selected males who had no degree of relatedness, from the five mesoregions of Goiás State. DNA was extracted from blood samples followed by the amplification of the 12 Y-chromosome loci. The products were analyzed to obtain the allele profiles on an ABI3500 automated sequencer using the Gene Mapper software. Allele frequencies and haplotype diversity were estimated by direct counting, and gene diversity for each locus was computed using the Arlequin software. The results are consistent with the history of miscegenation of the population of Central Brazil, in which we observed 321 different haplotypes. The average gene diversity at the 12 loci was 0.645. DYS385b and DYS389I showed the highest (0.704) and lowest (0.520) genetic diversity values, respectively. The F ST value between the Brazilian and Goiás populations was 0.00951, showing no statistical significance. The results of this study allowed the establishment of haplotypes found in the forensic samples of Goiás State serving as a reference in the elucidation of criminal cases and paternity tests, as well as population and evolutionary inferences.

show abstract

Allele frequencies of fifteen STR loci in a population from Central Brazil

Cited by 6 publications

References 3 publications

Avaliação de DNA polimerases em ensaios de amplificação de microssatélites através do PowerPlex®16 BIO System

Avaliação de DNA polimerases em ensaios de amplificação de microssatélites através do PowerPlex®16 BIO System

Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goiás, Central Brazil

Short Communication Y-STR haplotype diversity and population data for Central Brazil: implications for environmental forensics and paternity testing

Contact Info

Product

Resources

About