Allele frequencies for 22 autosomal short tandem repeat loci obtained by PowerPlex Fusion in a sample of 1501 individuals from the Japanese population

Fujii, Koji; Iwashima, Yasuki; Kitayama, Tetsushi; Nakahara, Hiroaki; Mizuno, Natsuko; Sekiguchi, Kazumasa

doi:10.1016/j.legalmed.2014.03.007

Cited by 27 publications

(12 citation statements)

References 11 publications

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“…Second-degree relatives are two meioses away from a particular individual in a pedigree (i.e., grandparent-grandchild, uncle/aunt-nephew/niece, and half-siblings). First, a series of genotypes in individuals #1-#4 were constructed on the basis of the allele frequencies of the 24 STR loci in the Japanese population [11][12][13]; then, those in individuals #5-#8 were constructed by mating of those of #1-#4. Mutational events were not considered in this process.…”

Section: Simulated Str Type Data For Putative Pedigreesmentioning

confidence: 99%

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

et al. 2015

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Section: Simulated Str Type Data For Putative Pedigreesmentioning

confidence: 99%

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

et al. 2015

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“…The allele 10 (U31Tdel) in the Sample A was originally found as a rare allele 9.3 at D5S818 locus in a population study of 1501 Japanese samples using PowerPlex ® Fusion . We have just finished the population study of GlobalFiler ® using the same 1501 samples.…”

Section: Resultsmentioning

confidence: 99%

“…The stored DNA of the Sample A was used in this study. This DNA sample was extracted from liquid blood using a MagNA Pure LC instrument (Roche Diagnostics, Basel, Switzerland) for the population studies and its 1/10 solution diluted with low TE buffer (TE −4 ) was quantified in real‐time PCR assay using the 207‐bp amplicon at D17Z1 locus in the same method described previously in the comparison study of DNA extraction instruments . The sample was used with the approval of the ethics committee at our institute.…”

Section: Methodsmentioning

confidence: 99%

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D5S818 Typing Discrepancy Between PowerPlex^® Fusion and Other STR Kits Including GlobalFiler^® Caused by a One‐base Deletion in 31 Nucleotides Upstream of the Repeat Region

Fujii

Iwashima

Watahiki

et al. 2016

Journal of Forensic Sciences

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Short tandem repeat (STR) typing is widely used in forensic investigation. When the same DNA sample is analyzed with different STR typing kits, a typing discrepancy is occasionally observed. In this study, we examined the cause of a typing discrepancy in a sample at D5S818 locus. This sample was designated as 10, 12 using Identifiler(®) , Identifiler(®) Plus, GlobalFiler(®) , PowerPlex(®) 16HS, and PowerPlex(®) 18D, but as 9.3, 12 using PowerPlex(®) Fusion. Sequencing results indicated that the shorter allele in the sample had a deletion (U31Tdel) at 31 nucleotides upstream of the repeat region (AGAT)10 . This deletion was located in the binding site of the published D5S818 forward primer in PowerPlex(®) 16 and was only 9 and 11 nucleotides downstream of our estimated 5' end position of D5S818 forward primer in GlobalFiler(®) and PowerPlex(®) 18D, respectively. We also examined the effect of primer length on the heterozygous peak balance in this sample.

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“…Allele frequencies of the Brunei Darussalam Malay were compared with previously published data from Singapore and Malaysia Malay, East Timor population and Filipinos from the Philippines . Brunei Chinese were compared with previously published allele frequencies from Singapore and Malaysia Chinese, Hong Kong Chinese, Taiwanese, Koreans, and Japanese .…”

Section: Methodsmentioning

confidence: 99%

Optimization of a reduced volume PCR amplification for PowerPlex® Fusion kit using FTA™ cards and generation of population genetic data for Brunei population

et al. 2018

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The commercial PowerPlex® Fusion kit is an autosomal STR multiplex kit that has high discrimination power and is more informative in forensic, paternity, and relationship-testing cases. Key features of this multiplex system are the possibility to direct amplify FTA™ card punches as well as non-FTA cards and commonly used swabs; optimised inhibitor tolerance and high sensitivity generating full profiles from amount as little as 100 pg of human DNA. This study focused on the optimization of performance variables such as FTA™ punch sizes, reduced reaction volumes, and FTA™ purification reagent aiming to increase the analytical sensitivity, decrease the sample consumption, and cost effectiveness. LOD and LOQ values demonstrated high sensitivity of the PowerPlex® Fusion system. In addition, population databases of Brunei Malay and Chinese from the Brunei Darussalam were established, and parameters of forensic importance were calculated. Overall, the forensic parameters indicated an enhanced utility of the PowerPlex® Fusion kit for forensic evidence analysis and paternity testing in Brunei Malay and Chinese populations.

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Allele frequencies for 22 autosomal short tandem repeat loci obtained by PowerPlex Fusion in a sample of 1501 individuals from the Japanese population

Cited by 27 publications

References 11 publications

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

D5S818 Typing Discrepancy Between PowerPlex^® Fusion and Other STR Kits Including GlobalFiler^® Caused by a One‐base Deletion in 31 Nucleotides Upstream of the Repeat Region

Optimization of a reduced volume PCR amplification for PowerPlex® Fusion kit using FTA™ cards and generation of population genetic data for Brunei population

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Allele frequencies for 22 autosomal short tandem repeat loci obtained by PowerPlex Fusion in a sample of 1501 individuals from the Japanese population

Cited by 27 publications

References 11 publications

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

Evaluation of advanced multiplex short tandem repeat systems in pairwise kinship analysis

D5S818 Typing Discrepancy Between PowerPlex® Fusion and Other STR Kits Including GlobalFiler® Caused by a One‐base Deletion in 31 Nucleotides Upstream of the Repeat Region

Optimization of a reduced volume PCR amplification for PowerPlex® Fusion kit using FTA™ cards and generation of population genetic data for Brunei population

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Product

Resources

About

D5S818 Typing Discrepancy Between PowerPlex^® Fusion and Other STR Kits Including GlobalFiler^® Caused by a One‐base Deletion in 31 Nucleotides Upstream of the Repeat Region