Allele and Genotype Frequencies of the Polymorphic Methylenetetrahydrofolate Reductase and Lung Cancer in ther Jordanian Population: a Case Control Study

Yousef, Al-Motassem; Shomaf, Maha; Said, Ismail; Berger, Sondra H.; Ababneh, Nidaa A.; Diab, Ola; Obeidat, Nathir M.; Awidi, Abdallah

doi:10.7314/apjcp.2015.16.8.3101

Cited by 12 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Al-Motassem et al established distribution of MTHFR c.677 C>T polymorphic variants as follows: C/C 59.6%, C/T 33.0%, and T/T 7.4%. Authors announced that patients who have C/C polymorphic variant also have an increased risk for LC [ 57 ]. Similar results on MTHFR c.677 C>T SNP distribution in LC patients were showed in our study: C/C polymorphic variant was dominating in our samples, C/C, C/T, T/T polymorphisms frequencies were respectively 58.7%, 37.0%, and 4.4%.…”

Section: Discussionmentioning

confidence: 99%

Individual and combined effect ofTP53, MDM2, MDM4, MTHFR, CCR5,andCASP8gene polymorphisms in lung cancer

et al. 2017

View full text Add to dashboard Cite

Lung cancer (LC) is the second common and with the highest mortality oncological disease. Specific biomarkers for its diagnostics, treatment, and prognosis are still under the investigations. Aim of our study was to evaluate the relationship between the polymorphisms of TP53 pathway genes TP53, MDM2, MDM4, the polymorphisms of HPV-associated genes MTHFR, CASP8, CCR5, and HPV infection with survival of LC patients. SNPs were genotyped using PCR-RFLP. qRT-PCR was used to detect, identify, and quantify HPV. No statistically significant differences were detected between individual SNPs and patient survival with stage I-IV LC. Cluster analysis of SNPs in genes MDM4 A/A, CCR5 wt/Δ32, MTHFR C/T, MDM2 T/T showed possible association with the worse survival. Patients who were diagnosed with C/T polymorphic variant of gene MTHFR tend not to survive stage III-IV LC (P = .12). There is a tendency between MDM2 gene T/T variant and worse survival of patients diagnosed with late stage LC (P = .11). HPV infection is very rear among LC patients (3 of 92). Overall, there is a link, although statistically insignificant, between specific SNPs and LC patient survival frequency and time, meanwhile the combination of specific SNPs showed a statistically significant measure. In conclusion, we determined statistically significant (P = .04) link between the poor survival of LC patients after surgery and the combination of polymorphic variants C/T of the MTHFR and T/T of the MDM2 genes, whereas individually these SNPs do not show significant relationship with the survival of patients after surgery.

show abstract

Section: Discussionmentioning

confidence: 99%

Individual and combined effect ofTP53, MDM2, MDM4, MTHFR, CCR5,andCASP8gene polymorphisms in lung cancer

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Recent studies show that the 677 and 1298 polymorphisms could be a risk factor for different type of cancer (ARAUJO et al 2015, AL-MOTASSEM et al 2015, and could also influence the radiotherapy treatments in patients with head and neck squamous cell carcinoma, presumably through the role of MTHFR in DNA methylation (FRISO et al 2012, JONES 2015, ANDERS et al 2015.…”

Section: Discussionmentioning

confidence: 99%

“…The focus of many studies since 1995 has been the association between the 677 C>T polymorphism, plasma homocysteine concentrations, and various clinical conditions: cardiovascular diseases-CVD (FROSST et al 1995, CHRISTENSEN et al 1997, SAFFARI et al 2013, HANSON et al 2001, vein thrombosis (DJORDJEVIC et al 2011), and neural tube closure defects (VAN DER PUT et al 1998, BEAUDIN andSTOVER, 2009). More recently, the findings that individuals homozygous for 677TT exhibit lower levels of genomic DNA methylation under low folate status (FRISO et al 2012) and that global DNA hypomethylation is observed in early tumorogenesis (JONES 2005), suggested that the 677 C>T mutation could also be connected with the development of cancer (ARAUJO et al 2015, AL-MOTASSEM et al 2015.…”

Section: Introductionmentioning

confidence: 99%

Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?

Djurovic

Stojković

Todorovic³

et al. 2017

Genetika

View full text Add to dashboard Cite

Methylenetetrahydrofolate reductase (MTHFR) plays a critical role in the folate metabolism. The polymorphism 677C>T of the MTHFR gene, producing thermolabile enzyme with decreased function, is widely studied and associated with many conditions. Additionally, it has been shown that another polymorphism, 1298A>C, also reduces the activity of this enzyme, although to a lesser extent. The aim of this study is to evaluate the clinical informativeness of testing both MTHFR polymorphisms. Genomic DNA, were extracted from peripheral blood of 180 female patients with pregnancy complications and 183 healthy female controls, and genotyped for MTHFR 677C>T and 1298A>C loci, using TaqMan assays. Our study found similar frequency of alleles and genotypes between two groups. Based on MTHFR 677C>T genotype, 11.7% of patients homozygous for this mutation were under the possible risk. When the position 1298 was included in the testing, 22.8% of the patients were heterozygous for both polymorphisms. Additionally, 8.9% of the patients were homozygous only for the MTHFR 1298 mutation. Although, there was no differences compared to healthy control (p>0.05), 43% of patients were found to have elevated risk which is about four time higher than results with only MTHFR 677C>T genotyping. After obtaining information for the 677 position, testing for the second polymorphism (1298A>C) should be 378

show abstract

“…However, the risk of population stratification in this study is unlikely because the Jordanian population is considered to be a genetically homogeneous population. Jordanians are descended from villagers and a Bedouin lineage originating in the Arabian Peninsula (27). Notably, the majority of the population is Arab (nearly 98%), and the remaining 2% is divided equally into Armenians and Circassians.…”

Section: Discussionmentioning

confidence: 99%

Genetic association analysis of ERBB4 polymorphisms with the risk ofschizophrenia susceptibility in a Jordanian population of Arab descent

2017

View full text Add to dashboard Cite

Background/aim: The ERBB4 gene encodes a transmembrane tyrosine kinase and is considered to be one of the risk genes of schizophrenia. Although there is evidence of the roles of genes and the environment in the etiology of schizophrenia, a comprehensive biological and genetic background of the disease is still lacking. The aim of this study is to assess whether genetic variation in the human ERBB4 gene is associated with vulnerability to schizophrenia in the Jordanian Arab population. Materials and methods:A total of 185 inpatients with schizophrenia participated in this study and 195 healthy genetically homogeneous individuals were also used as controls. Two genetic variants, rs839523 (G/A, intron 2) and rs3748962 (A/G, exon 27), encompassing the ERBB4 gene were genotyped using DNA sequencing. Results:The results revealed a strong and statistically significant genetic association of rs839523 with schizophrenia (P = 0.002 for allele and P = 0.006 for genotype). Conclusion:This study provides strong statistical evidence that there is an association between the ERBB4 gene and schizophrenia in a Jordanian population of Arab descent.

show abstract

Allele and Genotype Frequencies of the Polymorphic Methylenetetrahydrofolate Reductase and Lung Cancer in ther Jordanian Population: a Case Control Study

Cited by 12 publications

References 47 publications

Individual and combined effect ofTP53, MDM2, MDM4, MTHFR, CCR5,andCASP8gene polymorphisms in lung cancer

Individual and combined effect ofTP53, MDM2, MDM4, MTHFR, CCR5,andCASP8gene polymorphisms in lung cancer

Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?

Genetic association analysis of ERBB4 polymorphisms with the risk ofschizophrenia susceptibility in a Jordanian population of Arab descent

Contact Info

Product

Resources

About