Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta

Bhaskaran, G.; Dahm, Karl H.; Barrera, Punnee; Pacheco, J.; Peck, Kenneth E.; Muszyńska-Pytel, M.

doi:10.1016/0016-6480(90)90053-o

Cited by 40 publications

(14 citation statements)

References 30 publications

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“…Na-acetylation of ASB2 caused a small loss of activity, but it partially restored that of the truncated (9-18), (10)(11)(12)(13)(14)(15)(16)(17)(18), and (11-18) ASB2 amides (Table 1). Apparently, the greater the formal positive charge on these partial sequences, the greater the loss of bioactivity and the greater the extent of restoration by Na-acetylation.…”

Section: Resultsmentioning

confidence: 99%

“…They have been studied mostly for their action on glands from adult females in relation to the reproductive cycle (5)(6)(7)(8) but are also reported to act on larval CA (5) and, hence, may also have a role in metamorphosis. Compounds with similar function probably exist in other insects (9,10). The primary structures of an octapeptide amide and tridecapeptide amide from brains of Diploptera punctata were independently de-termined in two laboratories (5-7), and related nonapeptide and decapeptide amides have also been found (5).…”

mentioning

confidence: 99%

“…Thus, the endocrine message is located at the C terminus. Na-acetylation of the N-truncated (9)(10)(11)(12)(13)(14)(15)(16)(17)(18), (10)(11)(12)(13)(14)(15)(16)(17)(18), and (11)(12)(13)(14)(15)(16)(17)(18) fragments of ASB2 increases activity relative to the nonacetylated peptides. The site ofaction of type A and type B allatostatins is located before mevalonate kinase in the biosynthetic pathway for juvenile hormone.…”

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confidence: 99%

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Identity of a second type of allatostatin from cockroach brains: an octadecapeptide amide with a tyrosine-rich address sequence.

Pratt

Farnsworth

Fok

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

167

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An octadecapeptide that inhibits juvenile hormone synthesis has been isolated by HPLC from brainretrocerebral complexes of the cockroach Diploptera punctata. The primary structure of this allatostatin has been elucidated by tandem mass spectrometry: Ala-Tyr-Ser-Tyr-Val-Ser-GluTyr-Lys-Arg-Leu-Pro-Val-Tyr-Asn-Phe-Gly-Leu-NH2 (ASB2). The amidated three-residue C terminus of this type B allatostatin is identical to that of four known type A allatostatins, and the preceding three residues show close structural homology. ASB2 has over twice the activity of the type A tridecapeptide Ala-Pro-Ser-Gly-Ala-Gln-Arg-Leu-Tyr-GlyPhe-Gly-Leu-NH2 (ASA1) in inhibiting juvenile hormone biosynthesis in corpora allata from females in early vitellogenesis (day 2), and its efficacy persists during pregnancy, but it is equally effective as ASA1 on glands from day-10 females (IC50 = 0.31 nM). The octadecapeptide is characterized by a potential dibasic cleavage site, Lys'-Arg"', the integrity of which is needed for high potency. The ASB2-(11-18)-octapeptide amide gives a full response at high concentrations at day 10 (ICso = 48 nM), but the C-truncated (1-9)-, (1-11)-, and (1-17)-amide fragments of ASB2 are inactive. Thus, the endocrine message is located at the C terminus. Na-acetylation of the N-truncated (9-18), (10-18), and (11-18) fragments of ASB2 increases activity relative to the nonacetylated peptides. The site ofaction of type A and type B allatostatins is located before mevalonate kinase in the biosynthetic pathway for juvenile hormone.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Identity of a second type of allatostatin from cockroach brains: an octadecapeptide amide with a tyrosine-rich address sequence.

Pratt

Farnsworth

Fok

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

167

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“…Thirty thousand trimmed heads of M. sexta (fresh weight, "'.1460 g) were processed in three batches of 10 20-26% (vol/vol) 1-propanol in 0.5% heptafluorobutyric acid at a flow rate of S ml/min; 10-ml fractions were collected. Mas-AS activity was present in fractions that eluted at 48-60 min.…”

Section: Methodsmentioning

confidence: 99%

“…[4][5][6][7][8] or inhibitory [allatostatins (ASs), e.g., refs. [9][10][11][12][13][14][15][16][17]. It has been widely acknowledged that the design of agents that antagonize and/or mimic the mode of action of insect neuropeptides could lead to environmentally sound insect control strategies (18,19).…”

mentioning

confidence: 99%

Identification of an allatostatin from the tobacco hornworm Manduca sexta.

Kramer¹,

Toschi²,

Miller³

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

263

165

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A peptide (Manduca sexta ailatostatin) that strongly inhibits juvenile hormone biosynthesis in vitro by the corpora allata from fifth-stadium larvae and adult females has been purified from extracts of heads of pharate adult M. sexta by a nine-step purification procedure. The primary structure of this 15-residue peptide has been determined: pGlu-Val-ArgPhe-Arg-Gln-Cys-Tyr-Phe-Asn-Pro-Ile-Ser-Cys-Phe-OH, where pGlu is pyroglutamate). To our knowledge, this neurohormone has no sequence similarity with any known neuropeptide from other organisms. The synthetic free acid and amide forms showed in vitro activity indistihable from that of native M. sexta aliatostatn. The EDsO of synthetic M. sexta allatostatin on early fifth stadium larval corpora allata in vitro was =2 nM. This inhibition was reversible. In a cross-species study, M. sexta allatostatin also inhibited the corpora allata of adult female Heliothis virescens but had no effect on the activity of corpora allata ofadult females of the beetle Tenebrio molitor, the grasshopper Melanoplus sanguinipes, or the cockroach Periplaneta americana.Juvenile hormone (JH), which is synthesized and released by the retrocerebral corpora allata (CA), plays a vital role in insect development, primarily in the control of metamorphosis, adult sexual maturation, and reproduction (1, 2). Environmental and physiological factors influence neurosecretory centers in the brain that affect the activity of CA through peptidergic materials either transported directly to the CA by axons or released into the blood (3). These neuropeptides may be stimulatory (allatotropins, e.g., refs. 4-8) (27). However, instead of assaying for JH production by chloroform extraction followed by TLC analysis, we used a rapid isooctane partitioning assay for JH (28). In the cross-species studies, we used the same in vitro technique (one or two pair of CA per incubation).Extraction and Preliminary Purification (Steps 14). Thirty thousand trimmed heads of M. sexta (fresh weight, "'.1460 g) were processed in three batches of 10,000. Each batch was defatted by homogenizing in 2 liters of ice-cold acetone and filtered. Residues were extracted with 1.4 liters of 1 M HOAc/20 mM HCO (containing 0.1 mM phenylmethylsulfonyl fluoride and 0.01 mM pepstatin A, prepared immediately before use) and centrifuged at 10,000 x g for 30 min at 40C. The pellet was extracted and centrifuged twice using a total volume of 2.8 liters of the same solution. The combined supernatants were stirred with swollen SP-Sephadex C-25 resin (300 ml) overnight. The resin was allowed to settle for 2 hr, subsequently poured into a Bio-Rad column (50 x 300 mm), and equilibrated with 1 M HOAc. Material was eluted from the column sequentially with 1-liter volumes of 0.05 M NH4OAc (pH 4.0) and 0.05 M, 0.1 M, 0.2 M, 0.4 M, and 0.8 M NH4OAc (pH 7.0). The 0.1 M and 0.2 M fractions, which had M. sexta AS (Mas-AS) activity, were applied directly to 10 g of reversed-phase Vydac C4 packing material (20-30 pAm, contained in a 75-ml polypropylene syr...

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Expression of theManduca sexta allatotropin gene in cells of the central and enteric nervous systems

Bhatt

Horodyski

1999

J. Comp. Neurol.

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Allatinhibin, a neurohormonal inhibitor of juvenile hormone biosynthesis in Manduca sexta

Cited by 40 publications

References 30 publications

Identity of a second type of allatostatin from cockroach brains: an octadecapeptide amide with a tyrosine-rich address sequence.

Identity of a second type of allatostatin from cockroach brains: an octadecapeptide amide with a tyrosine-rich address sequence.

Identification of an allatostatin from the tobacco hornworm Manduca sexta.

Expression of theManduca sexta allatotropin gene in cells of the central and enteric nervous systems

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