All0809/8/7 is a DevBCA-like ABC-type efflux pump required for diazotrophic growth in Anabaena sp. PCC 7120

Staron, Peter; Maldener, Iris

doi:10.1099/mic.0.058909-0

Cited by 11 publications

(12 citation statements)

References 43 publications

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“…PCC 7120 (encoded by the genes all0809 , all2675 , alr3647 , alr4280 , alr4973 , and all5347 ) also have cytoplasmic tails of 10–18 amino acids, but they are totally different in amino acid sequence. The protein All0809 is not able to promote a substrate‐dependent reaction of the ATPase activity of DevAC, although it binds to DevAC [35]. The cytoplasmic tail might add an additional binding‐specificity control of the MFP to the IMF and vice versa .…”

Section: Discussionmentioning

confidence: 99%

Structure–function analysis of the ATP‐driven glycolipid efflux pump DevBCA reveals complex organization with TolC/HgdD

2013

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a b s t r a c tIn Gram-negative bacteria, trans-envelope efflux pumps have periplasmic membrane fusion proteins (MFPs) as essential components. MFPs act as mediators between outer membrane factors (OMFs) and inner membrane factors (IMFs). In this study, structure-function relations of the ATPdriven glycolipid efflux pump DevBCA-TolC/HgdD from the cyanobacterium Anabaena sp. PCC 7120 were analyzed. The binding of the MFP DevB to the OMF TolC absolutely required the respective tip-regions. The interaction of DevB with the IMF DevAC mainly involved the b-barrel and the lipoyl domain. Efficient binding to DevAC and TolC, substrate recognition and export activity by DevAC were dependent on stable DevB hexamers. Structured summary of protein interactions:DevB binds to DevB by surface plasmon resonance (View interaction) DevC and DevA physically interact with DevB by surface plasmon resonance (View interaction) TolC binds to DevB by surface plasmon resonance (1, 2) DevB and DevB bind by molecular sieving (View interaction)

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Section: Discussionmentioning

confidence: 99%

Structure–function analysis of the ATP‐driven glycolipid efflux pump DevBCA reveals complex organization with TolC/HgdD

2013

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“…have been identified (Shvarev & Maldener, ; Staron, ). Some are important for diazotrophic growth and heterocyst maturation (Fan et al, ; Shvarev, Nishi, Wörmer, & Maldener, ; Staron & Maldener, ). The cluster all5347/all5346/all5345 ( hgdB/hgdC/hgdA ) is of particular interest because the ATPase‐coding gene devA is replaced by the hgdA gene coding for a putative epimerase.…”

Section: Introductionmentioning

confidence: 99%

Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP‐galactose 4‐epimerase HgdA

2019

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The nitrogenase complex in the heterocysts of the filamentous freshwater cyanobacterium Anabaenasp. PCC 7120 fixes atmospheric nitrogen to allow diazotrophic growth. The heterocyst cell envelope protects the nitrogenase from oxygen and consists of a polysaccharide and a glycolipid layer that are formed by a complex process involving the recruitment of different proteins. Here, we studied the function of the putative nucleoside‐diphosphate‐sugar epimerase HgdA, which along with HgdB and HgdC is essential for deposition of the glycolipid layer and growth without a combined nitrogen source. Using site‐directed mutagenesis and single homologous recombination approach, we performed a thoroughly functional characterization of HgdA and confirmed that the glycolipid layer of the hgdAmutant heterocyst is aberrant as shown by transmission electron microscopy and chemical analysis. The hgdA gene was expressed during late stages of the heterocyst differentiation. GFP‐tagged HgdA protein localized inside the heterocysts. The purified HgdA protein had UDP‐galactose 4‐epimerase activity in vitro. This enzyme could be responsible for synthesis of heterocyst‐specific glycolipid precursors, which could be transported over the cell wall by the ABC transporter components HgdB/HgdC.

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“…Several homologs of the devBCA gene cluster in the genome of Anabaena sp. have been identified (19, 20). Some are important for diazotrophic growth and heterocyst maturation (21– 23).…”

Section: Introductionmentioning

confidence: 99%

“…have been identified (19, 20). Some are important for diazotrophic growth and heterocyst maturation (21– 23). The cluster all5347/all5346/all5345 ( hgdB/hgdC/hgdA ) is of particular interest because the ATPase-coding gene devA is replaced by the hgdA gene coding for a putative epimerase.…”

Section: Introductionmentioning

confidence: 99%

Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP-galactose 4-epimerase HgdA

Shvarev

Nishi

Maldener

2018

Preprint

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27The nitrogenase complex in the heterocysts of the filamentous freshwater cyanobacterium 28Anabaena sp. PCC 7120 fixes atmospheric nitrogen to allow diazotrophic growth. The heterocyst 29 cell envelope protects the nitrogenase from oxygen and consists of a polysaccharide and a 30 glycolipid layer that are formed by a complex process involving the recruitment of different 31 proteins. Here we studied the function of the putative nucleoside-diphosphate-sugar epimerase 32HgdA, which along with HgdB and HgdC is essential for deposition of the glycolipid layer and 33 growth without a combined nitrogen source. Using site-directed mutagenesis and single 34 homologous recombination approach, we performed a thoroughly functional characterization of 35HgdA and confirmed that the glycolipid layer of the hgdA mutant heterocyst is aberrant as shown 36 by transmission electron microscopy and chemical analysis. The hgdA gene was expressed 37 during late stages of the heterocyst differentiation. GFP-tagged HgdA protein localized inside the 38 heterocysts. The purified HgdA protein had UDP-galactose 4-epimerase activity in vitro. This 39 enzyme could be responsible for synthesis of heterocyst-specific glycolipid precursors, which 40 could be transported over the cell wall by the ABC transporter components HgdB/HgdC. 41 42

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All0809/8/7 is a DevBCA-like ABC-type efflux pump required for diazotrophic growth in Anabaena sp. PCC 7120

Cited by 11 publications

References 43 publications

Structure–function analysis of the ATP‐driven glycolipid efflux pump DevBCA reveals complex organization with TolC/HgdD

Structure–function analysis of the ATP‐driven glycolipid efflux pump DevBCA reveals complex organization with TolC/HgdD

Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP‐galactose 4‐epimerase HgdA

Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP-galactose 4-epimerase HgdA

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