All-trans retinoic acid stimulates the secretion of TGF-β2 via the phospholipase C but not the adenylyl cyclase signaling pathway in retinal pigment epithelium cells

Zhang, Daren; Deng, Zhihong; Tan, Jia; Liu, Shuirong; Hu, Shuyu; Tao, Hui; Tang, Renhong

doi:10.1186/s12886-018-1017-6

Cited by 10 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, with further development of myopia, axial lengthening of the eye can lead to loss of epithelial integrity, ultimately resulting in degeneration of the choroid and retina. Additionally, atRA promotes the secretion of TGF-β in RPE through the phospholipase C pathway [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

No Evidence of an Association between Genetic Factors Affecting Response to Vitamin A Supplementation and Myopia: A Mendelian Randomization Study and Meta-Analysis

Xu,

Liu,

2024

Nutrients

View full text Add to dashboard Cite

The relationship between vitamin A supplementation and myopia has been a topic of debate, with conflicting and inconclusive findings. We aimed to determine whether there is a causal relationship between vitamin A supplementation and the risk of myopia using Mendelian randomization (MR) and meta-analytical methods. Genetic variants from the UK Biobank and FinnGen studies associated with the response to vitamin A supplementation were employed as instrumental variables to evaluate the causal relationship between vitamin A supplementation and myopia. Fixed-effects meta-analysis was then used to combine MR estimates from multiple sources for each outcome. The meta-analysis of MR results found no convincing evidence to support a direct causal relationship between vitamin A supplementation and myopia risk (odds ratio (OR) = 0.99, 95% confidence interval (CI) = 0.82–1.20, I2 = 0%, p = 0.40). The analysis of three out of the four sets of MR analyses indicated no direction of causal effect, whereas the other set of results suggested that higher vitamin A supplementation was associated with a lower risk of myopia (OR = 0.002, 95% CI 1.17 × 10−6–3.099, p = 0.096). This comprehensive MR study and meta-analysis did not find valid evidence of a direct association between vitamin A supplementation and myopia. Vitamin A supplementation may not have an independent effect on myopia, but intraocular processes associated with vitamin A may indirectly contribute to its development.

show abstract

Section: Discussionmentioning

confidence: 99%

No Evidence of an Association between Genetic Factors Affecting Response to Vitamin A Supplementation and Myopia: A Mendelian Randomization Study and Meta-Analysis

Xu,

Liu,

2024

Nutrients

View full text Add to dashboard Cite

show abstract

“…Through the miR-328 -PAX6 axis, exogenous atRA could upregulate metalloproteinase-2 and downregulate collagen I and integrin β 1 in the scleral cells [ 38 ]. In addition, atRA could stimulate retinal pigment epithelium to secret TGF-β, another strong extracellular matrix remodeling promoter [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Screening for novel risk factors related to high myopia using machine learning

et al. 2022

View full text Add to dashboard Cite

Background High myopia-related complications have become a major cause of irreversible vision loss. Evaluating the association between potential factors and high myopia can provide insights into pathophysiologic mechanisms and further intervention targets for myopia progression. Method Participants aged 12–25 years from National Health and Nutrition Examination Survey 2001–2006 were selected for the analysis. Myopia was defined as spherical equivalent (sum of spherical error and half of the cylindrical error) of any eyes ≤-0.5 diopters. High myopia was defined as the spherical equivalent of any eye ≤ − 5.00 diopters. Essential variables were selected by Random Forest algorithm and verified by multivariable logistic regression. Results A total of 7,033 participants and 74 potential factors, including demographic (4 factors), physical examination (6 factors), nutritional and serological (45 factors), immunological (9 variables), and past medical history factors (10 factors), were included into the analysis. Random Forest algorithm found that several anthropometric, nutritional, and serological factors were associated with high myopia. Combined with multivariable logistic regression, high levels of serum vitamin A was significantly associated with an increased prevalence of high myopia (adjusted odd ratio = 1.46 for 1 µmol/L increment, 95% confidence interval [CI] 1.01–2.10). Furthermore, we found that neither C-reactive protein nor asthma increased the risk and severity of myopia. Conclusion High levels of serum vitamin A was seemingly associated with an increased prevalence of high myopia. This borderline significant association should be interpreted with caution because the potential increased type I error after the multiple testing. It still needs further investigation regarding the mechanism underlying this association. Neither C-reactive protein nor asthma increased the risk and severity of myopia.

show abstract

“…Primary human pigmented epithelial cells (HRPEs) were purchased from Angio-Proteomie (cAP-0037, Boston, MA, USA) and maintained in HRPECs Growth Medium (contains serum and growth supplements, cAP-33) following the manufacturer’s instructions. HRPE cells were plated in a 6-well plate and cultured for 24 h. Cells were washed once with PBS before being treated with MEM media (Lonza Bioscience) containing retinoic acid, dexamethasone, or vehicle control dimethyl sulfoxide (DMSO) for another 24 h 93 . Cells were washed three times with PBS before being lysed for total RNA extraction using NucleoSpin RNA, Mini Kit (Macherey-Nagel, Duren, Germany) following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Contribution of common and rare variants to Asian neovascular age-related macular degeneration subtypes

Fan,

Li,

Wang

et al. 2023

Nat Commun

View full text Add to dashboard Cite

Neovascular age-related macular degeneration (nAMD), along with its clinical subtype known as polypoidal choroidal vasculopathy (PCV), are among the leading causes of vision loss in elderly Asians. In a genome-wide association study (GWAS) comprising 3,128 nAMD (1,555 PCV and 1,573 typical nAMD), and 5,493 controls of East Asian ancestry, we identify twelve loci, of which four are novel ($$P \, < \, 1.19\times {10}^{-8}$$ P < 1.19 × 10 − 8 ). Substantial genetic sharing between PCV and typical nAMD is noted (rg = 0.666), whereas collagen extracellular matrix and fibrosis-related pathways are more pronounced for PCV. Whole-exome sequencing in 259 PCV patients revealed functional rare variants burden in collagen type I alpha 1 chain gene (COL1A1; $$P=1.05\times {10}^{-6}$$ P = 1.05 × 10 − 6 ) and potential enrichment of functional rare mutations at AMD-associated loci. At the GATA binding protein 5 (GATA5) locus, the most significant GWAS novel loci, the expressions of genes including laminin subunit alpha 5 (Lama5), mitochondrial ribosome associated GTPase 2 (Mtg2), and collagen type IX alpha 3 chain (Col9A3), are significantly induced during retinal angiogenesis and subretinal fibrosis in murine models. Furthermore, retinoic acid increased the expression of LAMA5 and MTG2 in vitro. Taken together, our data provide insights into the genetic basis of AMD pathogenesis in the Asian population.

show abstract

All-trans retinoic acid stimulates the secretion of TGF-β2 via the phospholipase C but not the adenylyl cyclase signaling pathway in retinal pigment epithelium cells

Cited by 10 publications

References 26 publications

No Evidence of an Association between Genetic Factors Affecting Response to Vitamin A Supplementation and Myopia: A Mendelian Randomization Study and Meta-Analysis

No Evidence of an Association between Genetic Factors Affecting Response to Vitamin A Supplementation and Myopia: A Mendelian Randomization Study and Meta-Analysis

Screening for novel risk factors related to high myopia using machine learning

Contribution of common and rare variants to Asian neovascular age-related macular degeneration subtypes

Contact Info

Product

Resources

About