All- trans -retinoic acid represses chemokine expression in adipocytes and adipose tissue by inhibiting NF-κB signaling

Karkeni, Esma; Bonnet, L.; Astier, Julien; Couturier, Cyril; Dalifard, Julie; Tourniaire, Franck; Landrier, Jean‐François

doi:10.1016/j.jnutbio.2017.01.004

Cited by 40 publications

(32 citation statements)

References 46 publications

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“…Total cellular RNA was extracted using TRIzol reagent according to the manufacturer's instructions. cDNA was synthesized from 1 µg of total RNA using random primers and Moloney murine leukemia virus reverse transcriptase . Real‐time quantitative RT‐PCR analyses were performed using the Mx3005P Real‐Time PCR System (Stratagene, La Jolla, CA) as previously described .…”

Section: Methodsmentioning

confidence: 99%

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(all‐E)‐ and (5Z)‐Lycopene Display Similar Biological Effects on Adipocytes

Fenni

Astier

Bonnet

et al. 2018

Molecular Nutrition Food Res

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Scope Although about 90% of lycopene in dietary sources occurs in the linear all‐trans conformation, a large proportion of the lycopene found in human tissues is of the cis‐isomer type, notably (5Z)‐lycopene. The biological effects of this (5Z) isomer have been under‐researched. The aim of this study is to evaluate some biological functions of (5Z)‐lycopene in adipocytes and to compare them with those of (all‐E)‐lycopene. Methods and results (all‐E)‐ and (5Z)‐Lycopene displayed strong similarities in global gene expression profile and biological pathways impacted. Peroxisome proliferator‐activated receptor (PPAR) signaling is identified as a major actor mediating the effects of lycopene isomers. Transactivation assays confirmed the ability of both isomers to transactivate PPARγ. In addition, the TNFα‐induced proinflammatory cytokine mRNA expression in 3T3‐L1 adipocytes is reduced by both isomers via a reduction in the phosphorylation levels of p65. Finally, lycopene isomers restore the TNF‐α‐blunted uptake of glucose by adipocytes via a modulation of AKT phosphorylation. Conclusion These results show that lycopene isomers exert similar biological functions in adipocytes, linked to their ability to transactivate PPARγ. These findings add to our knowledge of lycopene effects in adipocyte biology and point to the possible use of lycopene in the prevention of obesity‐related disorders.

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Section: Methodsmentioning

confidence: 99%

“…RNA quality control was performed on an Agilent 2100 Bioanalyzer (Massy, France) with 6000 Nano Chips, according to the manufacturer's instructions and as previously reported . RNA from three independent experiments were pooled per treated group and hybridized to Agilent Whole Human Genome (4 × 44k; Massy, France).…”

Section: Methodsmentioning

confidence: 99%

(all‐E)‐ and (5Z)‐Lycopene Display Similar Biological Effects on Adipocytes

Fenni

Astier

Bonnet

et al. 2018

Molecular Nutrition Food Res

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“…The results suggest that ATRA may prevent the IκΒ degradation and increased expression of IKK induced by paclitaxel. It has been reported that ATRA inhibits NF‐κΒ activation in many cell types . In this study, treatment with ATRA was found to reduce NF‐κΒ expression in the nuclei of resistant cells, but not in those of parental colorectal cancer cells.…”

Section: Resultsmentioning

confidence: 52%

“…It has been reported that ATRA inhibits NF-κΒ activation in many cell types. 21,22 In this study, treatment with ATRA was found to reduce NF-κΒ expression in the nuclei of resistant cells, but not in those of parental colorectal cancer cells. Furthermore, Cx43 transfection does not obviously reduce NF-κΒ expression in the nuclei of resistant cells.…”

Section: High Levels Of Nf-κβ Activation In Paclitaxelresistant Celmentioning

confidence: 56%

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All‐trans retinoic acid reverses epithelial‐mesenchymal transition in paclitaxel‐resistant cells by inhibiting nuclear factor kappa B and upregulating gap junctions

Shi

Zheng

et al. 2018

Cancer Science

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Paclitaxel is a widely used chemotherapy drug, but development of resistance leads to treatment failure. Tumor cells that are treated with a sublethal dose of paclitaxel for a long period of time show the epithelial‐mesenchymal transition (EMT) phenotype, which leads to metastasis and resistance. All‐trans retinoic acid (ATRA) is always used in combination with paclitaxel and can reverse EMT in many types of cancer cells. The ability of ATRA to reverse EMT in chemoresistant cells is still unknown. In the present study, the ability of ATRA to reverse EMT in paclitaxel‐resistant cells was investigated. Three colorectal cancer cell lines, HCT116, LoVo and CT26, were treated with sublethal doses of paclitaxel to create resistant cell lines. Western blotting, immunocytochemistry, and “parachute” dye‐coupling assays showed that ATRA reverses EMT, inhibits nuclear factor kappa B (NF‐κΒ), and upregulates gap junctions in paclitaxel‐resistant cells. Scratch wound‐healing and Transwell assays showed that ATRA decreases the migration and invasion abilities of paclitaxel‐resistant cells. In addition, the CT26 cell line was used in the Balb/c pulmonary metastasis model to show that ATRA reduces metastasis of paclitaxel‐resistant cells in vivo. Given these data, ATRA may reverse EMT by inhibiting NF‐κΒ and upregulating gap junctions in paclitaxel‐resistant cells.

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Palovarotene inhibits connective tissue progenitor cell proliferation in a rat model of combat‐related heterotopic ossification

Wheatley

Cilwa

Dey

et al. 2017

Journal Orthopaedic Research

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Heterotopic ossification (HO) develops in the extremities of wounded service members and is common in the setting of high-energy penetrating injuries and blast-related amputations. No safe and effective prophylaxis modality has been identified for this patient population. Palovarotene has been shown to reduce bone formation in traumatic and genetic models of HO. The purpose of this study was to determine the effects of Palovarotene on inflammation, progenitor cell proliferation, and gene expression following a blast-related amputation in a rodent model (n = 72 animals), as well as the ability of Raman spectroscopy to detect early HO before radiographic changes are present. Treatment with Palovarotene was found to dampen the systemic inflammatory response including the cytokines IL-6 (p = 0.01), TNF-α (p = 0.001), and IFN-γ (p = 0.03) as well as the local inflammatory response via a 76% reduction in the cellular infiltration at post-operative day (POD)-7 (p = 0.03). Palovarotene decreased osteogenic connective tissue progenitor (CTP-O) colonies by as much as 98% both in vitro (p = 0.04) and in vivo (p = 0.01). Palovarotene treated animals exhibited significantly decreased expression of osteo- and chondrogenic genes by POD-7, including BMP4 (p = 0.02). Finally, Raman spectroscopy was able to detect differences between the two groups by POD-1 (p < 0.001). These results indicate that Palovarotene inhibits traumatic HO formation through multiple inter-related mechanisms including anti-inflammatory, anti-proliferative, and gene expression modulation. Further, that Raman spectroscopy is able to detect markers of early HO formation before it becomes radiographically evident, which could facilitate earlier diagnosis and treatment. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1135-1144, 2018.

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All- trans -retinoic acid represses chemokine expression in adipocytes and adipose tissue by inhibiting NF-κB signaling

Cited by 40 publications

References 46 publications

(all‐E)‐ and (5Z)‐Lycopene Display Similar Biological Effects on Adipocytes

(all‐E)‐ and (5Z)‐Lycopene Display Similar Biological Effects on Adipocytes

All‐trans retinoic acid reverses epithelial‐mesenchymal transition in paclitaxel‐resistant cells by inhibiting nuclear factor kappa B and upregulating gap junctions

Palovarotene inhibits connective tissue progenitor cell proliferation in a rat model of combat‐related heterotopic ossification

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