All-trans-Retinoic Acid Blocks Cell Cycle Progression of Human Ovarian Adenocarcinoma Cells at Late G1

Cell cycle genes as targets of retinoid induced ovarian tumor cell growth suppression

“…Effect of RA on steady state mRNA levels of cell cycle genes in CA-OV3 and SK-OV3 cells Previous work from our laboratory has shown that the ovarian carcinoma cell line CA-OV3 is sensitive to RA treatment while the SK-OV3 cell line is resistant (Wu et al, 1997a). In an attempt to identify potential growth regulatory targets of RA we initially compared changes in expression of a representative group of cell cycle genes in CA-OV3 and SK-OV3 cells with and without RA treatment.…”

“…Many laboratories including our own have shown that retinoids inhibit the growth of a wide variety of tumor cells including ovarian carcinoma cells both in vivo and in vitro (Caliaro et al, 1994;Chao et al, 1997;Grunt et al, 1991Grunt et al, , 1992a for review see Zhang et al, 2000). Previous studies from our laboratory have investigated the e ect of all-transretinoic acid (RA) on the growth of ovarian carcinoma cells (Wu et al, 1997a). The growth of the cell line CA-OV3 was found to be inhibited by RA treatment while the growth of SK-OV3 cells was not.…”

Synthetic retinoids as inducers of apoptosis in ovarian carcinoma cell lines

“…Since retinoid treatment inhibits the growth of a variety of epithelial cancers, such as ovarian carcinomas, retinoids have great promise in the area of cancer chemotherapy and chemoprevention (De Luca, 1991;Gudas et al, 1994;Moon et al, 1994;Wu et al, 1997aWu et al, ,b, 1998bZhang et al, 2000).…”

Modulation of retinoic acid receptor function alters the growth inhibitory response of oral SCC cells to retinoids

“…MTT assay was performed daily as described previously by Wu et al, 1997a. For the e ects of all-trans-RA on SCC-25 cells, SCC-25 cells overexpressing mutant RAR-b2(R269Q) or MSCVpac vector transduced SCC-25 cells were seeded in 96-well plates at a density of 4000 cells/well, and treated with all-trans-RA at concentrations ranging from 10 710 ± 10 76 M. Control cultures were treated with an equal volume of ethanol. MTT assay was performed on day 7 after treatment.…”

“…Retinoids have been demonstrated to inhibit development of a number of di erent types of tumors (Aylsworth et al, 1986;Munker et al, 1987) and to inhibit the growth of many human tumor cell lines (Wu et al, 1997a(Wu et al, ,b, 1998aHarant et al, 1993;Saunders et al, 1993;Grunt et al, 1992;Somay et al, 1991Somay et al, , 1992Cline and Rice, 1983;Kim et al, 1984;Reiss et al, 1985;Thatcher et al, 1985;Rubin and Rice, 1986;Lotan et al, 1987;Sakar and Das, 1988) including squamous cell carcinomas (SCCs) (Cline and Rice, 1983;Kim et al, 1984;Reiss et al, 1985;Thatcher et al, 1985;Rubin and Rice, 1986;Lotan et al, 1987;Sakar and Das, 1988). Recently, retinoids have been successfully used in vivo to prevent the progression of preneoplastic oral, bronchial, and head-and-neck lesions to frank malignant tumors Benner et al, 1994;Lotan et al, 1995).…”