Abstract. Eigen frequency is a very important parameter in a fiber optic gyroscope (FOG). To establish an accurate measurement of eigen frequency, we propose a new measurement method based on employing the square-wave, over-bias modulation with double eigen frequency. We investigate the proposed method both experimentally and theoretically. Experimental results show the measurement accuracy of eigen frequency is better than AE5 Hz, which satisfies the eigen frequency measurement of most FOGs. A theoretical model of the method gives a good explanation of the experimental results. The method is simple, low-cost, and easy to implement. In addition, it can greatly improve the accuracy of the eigen frequency measurement without needing additional hardware for existing gyro systems. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.