All-printed cell counting chambers with on-chip sample preparation for point-of-care CD4 counting

Wasserberg, Dorothee; Zhang, Xichen; Breukers, Christian; Connell, Bridgette Janine; Baeten, Ellen; Blink, Dorine van den; Benet, Èlia S O L À; Bloem, Andries C.; Nijhuis, Monique; Wensing, Annemarie M. J.; Terstappen, Leon W.M.M.; Beck, Markus

doi:10.1016/j.bios.2018.07.002

Cited by 13 publications

(16 citation statements)

References 55 publications

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“…While capacitance values extracted from the signal are very similar between the two measurement runs, absolute cell numbers differed. Differences in cell counting between cell chamber counting methods and flow cytometric analysis are also reported in literature for different applications [43,44]. Flow cytometry analyzes a high number of cells within a very short time and can distinguish between single, budding and dead cells.…”

Section: Impedance Measurement Atline At Stiegl Brewerymentioning

confidence: 99%

Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

Brunauer

Spadiut

Alfred³

et al. 2020

Chemosensors

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Electrochemical impedance spectroscopy is a powerful tool in life science for cell and pathogen detection, as well as for cell counting. The measurement principles and techniques using impedance spectroscopy are highly diverse. Differences can be found in used frequency range (β or α regime), analyzed quantities, like charge transfer resistance, dielectric permittivity of double layer capacitance and in off- or online usage. In recent contributions, applications of low-frequency impedance spectroscopy in the α regime were tested for determination of cell counts and metabolic burden in Escherichia coli and Saccharomyces cerevisiae. The established easy to use methods showed reasonable potential in the lab scale, especially for S. cerevisiae. However, until now, measurements for cell counts in food science are generally based on Thoma cell counting chambers. These microscopic cell counting methods decelerate an easy and quick prediction of yeast viability, as they are labor intensive and result in a time delayed response signal. In this contribution we tested our developed method using low frequency impedance spectroscopy locally at an industrial brewery propagation site and compared results to classic cell counting procedures.

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Section: Impedance Measurement Atline At Stiegl Brewerymentioning

confidence: 99%

Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

Brunauer

Spadiut

Alfred³

et al. 2020

Chemosensors

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“…Recently, a new technique of chip microfabrication based on the Inkjet printing method has been adapted to CD4+ T cells trapping [66]. Furthermore, by the same technique, hydrogel layers with embedded fluorophore-labeled antibodies were deposited on polymethyl methacrylate slides onto microfluidic chips.…”

Section: Microfluidic Tools For Monitoring Aids Progressionmentioning

confidence: 99%

“…After incubation, the chambers are imaged in a custom-built, wide field-of-view fluorescence imager, which also performs the automated image analysis, to count CD4+ T cells (number of cells per μL of blood). Again, this technique showed an excellent agreement with FCM (R 2 = 0.97; n = 57) [ 66 ].…”

Section: Microfluidics For Studying Hiv Infectionmentioning

confidence: 99%

Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection

Eid

Mougel

Socol

2020

Viruses

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HIV-1 is the causative agent of acquired immunodeficiency syndrome (AIDS). It affects millions of people worldwide and the pandemic persists despite the implementation of highly active antiretroviral therapy. A wide spectrum of techniques has been implemented in order to diagnose and monitor AIDS progression over the years. Besides the conventional approaches, microfluidics has provided useful methods for monitoring HIV-1 infection. In this review, we introduce continuous microfluidics as well as the fabrication and handling of microfluidic chips. We provide a review of the different applications of continuous microfluidics in AIDS diagnosis and progression and in the basic study of the HIV-1 life cycle.

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“…Application of microfabrication to study tissue- and organ-level processes (tissues-on-a-chip and organs-on-a-chip) is a growing field of research and could serve as a platform for carrying out tightly controlled, high-throughput drugs toxicity screening studies [8,35,36]. Besides controlling the cellular microenvironment, microfabrication and soft lithography techniques have found excellent uses in imaging biological specimens [37,38,39], cell counting and sorting [40,41,42,43,44,45,46,47], and engineering of micro bioreactors [48,49,50], amongst other applications. Furthermore, soft lithography have allowed to propose and develop revolutionary approaches for biomedical applications; one such example is its impact on diagnostics and global health care enabled by miniaturization, costs reduction and integration of multiple functionalities in portable and reliable platforms [51,52].…”

Section: Development Of Microfabrication For Biological Research: mentioning

confidence: 99%

Integrating Microfabrication into Biological Investigations: the Benefits of Interdisciplinarity

2019

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The advent of micro and nanotechnologies, such as microfabrication, have impacted scientific research and contributed to meaningful real-world applications, to a degree seen during historic technological revolutions. Some key areas benefitting from the invention and advancement of microfabrication platforms are those of biological and biomedical sciences. Modern therapeutic approaches, involving point-of-care, precision or personalized medicine, are transitioning from the experimental phase to becoming the standard of care. At the same time, biological research benefits from the contribution of microfluidics at every level from single cell to tissue engineering and organoids studies. The aim of this commentary is to describe, through proven examples, the interdisciplinary process used to develop novel biological technologies and to emphasize the role of technical knowledge in empowering researchers who are specialized in a niche area to look beyond and innovate.

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All-printed cell counting chambers with on-chip sample preparation for point-of-care CD4 counting

Cited by 13 publications

References 55 publications

Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection

Integrating Microfabrication into Biological Investigations: the Benefits of Interdisciplinarity

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