All-plastic, low-power, disposable, continuous-flow PCR chip with integrated microheaters for rapid DNA amplification

Moschou, Despina; Vourdas, N.; Kokkoris, George; Papadakis, George; Parthenios, John; Chatzandroulis, S.; Tserepi, Angeliki

doi:10.1016/j.snb.2014.04.007

Cited by 120 publications

(95 citation statements)

References 31 publications

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“…Such as simple substrate is ideal for commercial upscaling of LoC devices, since it benefits from the high degree of electronics integration, high precision, and the accumulated experience of several decades of industrial manufacturing [13,18]. Examples of PCBbased devices include micromixers [17], nucleic acid amplification chips [19] and chemical sensors [20].…”

Section: Page 2 Ofmentioning

confidence: 99%

Long-lasting FR-4 surface hydrophilisation towards commercial PCB passive microfluidics

Vasilakis

Moschou

Carta

et al. 2016

Applied Surface Science

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Highlights oxygen plasma treatment on FR-4 laminates  effect of power and exposure time studied to achieve long-lasting hydrophilic properties  super-hydrophilic surface for 13 days demonstrated  surface remains weakly hydrophilic between 13 th and 26 th day after the treatment  hydrophilic behaviour is retained for 26 days in total  oxygen plasma treatment was used on a commercially manufactured PCB microfluidic chip to enable passive filling of a microfluidic network AbstractPrinted circuit boards (PCB) technologies are an attractive system for simple sensing and microfluidic systems. Controlling the surface properties of PCB material is an important part of this technology and to date there has been no study on long-term hydrophilisation stability of these materials. In this work, the effect of different oxygen plasma input power and treatment duration times on the wetting properties of FR-4 surfaces was investigated by sessile droplet contact angle measurements. Super and weakly hydrophilic behaviour was achieved and the retention time of these properties was studied, with the hydrophilic nature being retained for at least 26 days. To demonstrate the applicability of this treatment method, a commercially manufactured microfluidic structure made from a multilayer PCB (3-layer FR-4 stack) was exposed to oxygen plasma at the optimum conditions. The structures could be filled with deionised (DI) water under capillary flow unlike the virgin devices.

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Section: Page 2 Ofmentioning

confidence: 99%

Long-lasting FR-4 surface hydrophilisation towards commercial PCB passive microfluidics

Vasilakis

Moschou

Carta

et al. 2016

Applied Surface Science

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“…(4)(5)(6)(7) PCR chips obtain information rapidly through the gene analysis of extremely small quantities of DNA, and thus can be used to diagnose diseases. (8)(9)(10)(11) The most important function of PCR chips is accurate temperature control. (12,13) However, research into their degree of accuracy has been limited.…”

Section: Introductionmentioning

confidence: 99%

Calibration of Temperature Sensors with Different Tolerances for Polymerase Chain Reaction Chips

2017

Sensors and Materials

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The calibration of temperature sensors with different tolerances is investigated to evaluate the effect of a polymerase chain reaction (PCR) on the performance of a PCR chip. Two types of thermistor sensors with 0.5 and 1% tolerances are applied to printed circuit board (PCB)-based PCR chips. The PCR performance is evaluated for calibrated and uncalibrated thermistors. The band volume variation of gel images is analyzed statistically to evaluate the performance. As a result of analyzing the band volume in gel images, the 0.5% thermistor was found to exhibit a level of performance that is almost equal in both the calibrated and uncalibrated thermistors.

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“…Starting from the early 90s a series of outstanding advances in microfluidics technology have been performed [1,2], enabling the research of fluid flows in the micro-and nano-scale [3], and inseminating a wide range of applications from chemical and biological analysis [4][5][6][7], to micro and nano synthesis routes [8]. Pertaining market analysis have shown the overall Compound Annual Growth Rate (CAGR), is expected to grow up to ~25% from 2010 to 2016, while the unit cost is decreasing to well below €5 (see ref.…”

Section: Introductionmentioning

confidence: 99%

uVALVIT: A tool for droplet mobility control and valving

Vourdas¹,

Dalamagkidis²,

Stathopoulos³

2016

MATEC Web of Conferences

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Abstract. Active control of droplet mobility through low cost tools is highly desirable in applications entailing microfluidics, Lab-on-Chip devices and pertinent technologies. Here, we present the design concepts of a versatile, low cost tool for dynamic droplet mobility manipulation, employing a scheme with backpressure application. Initially sticky open-or closed-channel fluidics with hydrophobic, porous walls are rendered slippery with the application of backpressure through the porous walls. Deliberate control of backpressure directs the wetting phenomena to the desired state. Operation parameters, and control system considerations are presented. Ultra-low backpressure values, are needed for channels with small cross-sections, which in turn are compatible with ultra-low energy demands.

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All-plastic, low-power, disposable, continuous-flow PCR chip with integrated microheaters for rapid DNA amplification

Cited by 120 publications

References 31 publications

Long-lasting FR-4 surface hydrophilisation towards commercial PCB passive microfluidics

Long-lasting FR-4 surface hydrophilisation towards commercial PCB passive microfluidics

Calibration of Temperature Sensors with Different Tolerances for Polymerase Chain Reaction Chips

uVALVIT: A tool for droplet mobility control and valving

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