All 4 Bile Salt Hydrolase Proteins Are Responsible for the Hydrolysis Activity in Lactobacillus plantarum ST‐III

Abstract

Hypercholesterolemia is considered one of the major risk factors for coronary heart disease. More interest has focused on intestinal microbes because of their role in the decrease of serum cholesterol. BSH proteins play an important role in the reduction of cholesterol. This paper adds to a better understanding of BSH proteins of intestinal microbes. It gives a great hint that probiotics can be used to solve hypercholesterolemia one day.

Identification and characterization of wild lactobacilli and pediococci from spontaneously fermented Mountain Cheese

“…All amino acids were purchased from Sigma. The capacity of isolated strains to hydrolyze bile salts was tested by using a plate assay according to the method previously described by Ren et al (2011), with some modifications. Each culture was spotted (10 mL) onto MRS agar plates supplemented with 0.5% (w/v) taurodeoxycholic acid (TDCA; Sigma) and 0.5% (w/v) glycodeoxycholic acid (GDCA; Sigma).…”

Determination of the essential nutrients required for milk fermentation by Lactobacillus plantarum

“…Certain L. plantarum strains may exhibit beneficial functions in vivo, such as the regulation of the immune system, inhibition of intestinal pathogens, and prevention of cardiovascular disease (Choi & Chang, 2015;Kawashima et al, 2011;Ren, Sun, Wu, Yao, & Guo, 2011). Therefore, these strains could potentially be used as functional probiotics.…”

Penicillin acylases revisited: importance beyond their industrial utility

“…Structures have been elucidated for BSH from Clostridium perferingens (Rossocha et al, 2005) and Bifidobacterium longum (Kumar et al, 2006). It is also noted that (Ren et al, 2011) most BSH occur in Gram positive bacteria (except Bacteroides), while PVAs occur widely in all bacteria. Unlike PVA, the occurrence of multiple BSH homologues in the same bacterium has also been reported.…”