Alkohol und Blut

Peroxo Compounds of Vanadium(V) and Niobium(V) as Potent Inhibitors of Calcineurin Activity towards RII‐Phosphopeptide

“…It is worth noting that each of the inhibitor species including H 2 O 2 inhibited the CN activity towards RII-phosphopeptide to nearly the same extent as its p-NPP phosphatase activity with comparable IC 50 values [ Figure 7 and Figure S6 (Supplementary Information) and Table 3]. The IC 50 value of 32.5 mM obtained for H 2 O 2 in the present study is in good agreement with the IC 50 value of 30-40 mM previously reported by Reiter and Rusnak, [18,19] for inhibition of intracellular CN activity by H 2 O 2 in T-lymphocytes. The IC 50 for polymeric compounds is reported on the basis of their actual peroxometal loading.…”

“…[4][5][6][8][9][10][11][12][13][14][15][16][17] Interestingly, a number of groups provided evidence that CN activity can be inhibited by extracellular oxidants, in particular H 2 O 2 . [17][18][19][20][21][22] However, there has been conflicting conclusions about mechanism of such inhibition. [17][18][19][20][21][22] In a previous investigation, a synthetic peroxovanadate compound was also used to study the effect of oxidative stress on calcineurin activity [22] whereas another report showed that orthovanadate inhibits protein serine/threonine phosphatase activity of calcineurin.…”

“…[17][18][19][20][21][22] However, there has been conflicting conclusions about mechanism of such inhibition. [17][18][19][20][21][22] In a previous investigation, a synthetic peroxovanadate compound was also used to study the effect of oxidative stress on calcineurin activity [22] whereas another report showed that orthovanadate inhibits protein serine/threonine phosphatase activity of calcineurin. [12] Stabilized diperoxovanadate has already been recognized as a useful tool in investigations of cellular effects mediated by H 2 O 2 .…”