ALKBH5 Is Lowly Expressed in Esophageal Squamous Cell Carcinoma and Inhibits the Malignant Proliferation and Invasion of Tumor Cells

Abstract

Background. Modification of N6-methyladenosine (m6A) and RNA m6A regulatory factors is required in cancer advancement. The contribution of m6A and its alteration in esophageal squamous cell carcinoma (ESCC) is still unclear. Results. ALKBH5 was lowly expressed in ESCC tissues, which the total m6A level was increased in ESCC tissue than the presentation in normal healthy tissue. The pcDNA3.1-ALKBH5 recombinant plasmid was transfected into KYSE-150 and Eca-109 cells. The overexpression of ALKBH5 is responsible for a significant reduction of the total m6A levels in Eca-109 and KYSE150 cells, inhibiting the proliferation capability, migration, and cell invasion. Conclusions. ALKBH5 as a demethylase was lowly expressed in cancer progression of ESCC and acts as a crucial component in ESCC progression.

Role of N6-Methyladenosine Methylation Regulators in the Drug Therapy of Digestive System Tumours

“…ALKBH5, an m6A regulatory factor, is down-regulated in esophageal cancer. The overexpression of ALKBH5 inhibits esophageal cancer cell proliferation and promotes ESCC cell apoptosis ( Li et al, 2021a ). Liu et al revealed a METTL14-miR-99a-5p-TRIB2 positive feedback loop in esophageal cancer that enhances tumour stem cell characterisation and drug resistance of ESCC cells.…”

The Role of m6A Modification and m6A Regulators in Esophageal Cancer

“…Most studies revealed that the m 6 A level was elevated in ESCC tissues and cell lines [ 73 , 84 , 85 , 86 ]. However, Cui et al [ 83 ] found lower m 6 A levels in ESCC cell lines and tissues compared to their counterparts.…”

“…The expression, clinical significance and biological function of m 6 A regulators in ESCA are shown in Supplementary Table S1 [ 59 , 60 , 61 , 66 , 69 , 72 , 73 , 74 , 75 , 76 , 79 , 80 , 83 , 84 , 85 , 86 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 ].…”

“…The expression of ALKBH5 was downregulated in ESCA tissues [ 80 , 86 , 122 , 130 , 131 ], which was more frequently observed in advanced ESCC such as T3–T4, N1–N3, clinical stage III–IV and histological grade III tumors [ 122 , 130 , 131 ]. FTO was reported to be increased in ESCA tissues and cell lines in most studies [ 73 , 74 , 83 , 132 ].…”

“…The m 6 A regulators affected the biological behavior of ESCC cells through the same or a different gene/signaling pathway, which is shown in Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 [ 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 124 , 126 , 127 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 141 ].…”