Alkaloids with neuroprotective effects from the leaves of Isatis indigotica collected in the Anhui Province, China

Alkaloid Enantiomers from Isatis tinctoria with Neuroprotective Effects against H2O2-Induced SH-SY5Y Cell Injury

“…The neuroprotective activities of all compounds on human neuroblastoma SH-SY5Y cell injury induced by H 2 O 2 were measured according to a published method [15]. Cells were seeded at 96-well culture plates (1 × 10 4 cells/well) in 200 µL of medium.…”

“…Previous chemical investigations of I. tinctoria have shown a wide range of natural products, including alkaloids [3][4][5][6], lignans [7,8], flavonoids [9], ceramides [10], and organic acids [11,12]. Biological research has reported that these compounds have a broad range of activities, such as cytotoxic, anti-inflammatory, antiviral, and neuroprotective activities [13][14][15]. In the medicinal plant I. tinctoria, alkaloids are one of the characteristic and active components.…”

“…Therefore, it is essential to evaluate the bioactivity of enantiomers. As part of our continuing efforts to discover compounds with potent neuroprotective activity from I. tinctoria [15,[21][22][23], six pairs of alkaloid enantiomers including 11 new alkaloids (1a/1b-5a/5b, 6a) (▶ Fig. 1) were obtained.…”

Ecological Roles of Tryptanthrin, Indirubin and N‐Formylanthranilic Acid in Isatis indigotica: Phytoalexins or Phytoanticipins?

“…(Chinese woad), a biennial crucifer widely cultivated and used in traditional Chinese medicine, [2] and I. tinctoria L., an indigo dye plant, produce a number of metabolites with various biological activities. [2,3] Interestingly, air drying leaves of I. tinctoria and I. indigotica was shown to change their metabolite profiles causing the loss of glucosinolates and indigo precursors and the formation of tryptanthrin (1) and indirubin (2; Figure 1). [4] Both tryptanthrin (1) and indirubin (2) are compounds well-known for their pharmacological activities including antimicrobial, anti-inflammatory and anticancer.…”

“…[4] Both tryptanthrin (1) and indirubin (2) are compounds well-known for their pharmacological activities including antimicrobial, anti-inflammatory and anticancer. [3,[5][6][7][8] Nonetheless, their roles in the producing organisms remain unknown to date.…”