Alkaloids from Rhazya stricta

Atta-ur-Rahman, _; Zaman, Khurshid; Perveen, Shahnaz; Habib-ur-Rehman, _; Muzaffar, Anium; Choudhary, M. Iqbal; Pervin, Azra

doi:10.1016/s0031-9422(00)95218-x

Cited by 46 publications

(19 citation statements)

References 39 publications

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“…These data were in accordance with those reported by Atta-ur-Rahman et al (1991) except the slight maximum at 298 nm and at 305.…”

Section: Plant Cell Repsupporting

confidence: 93%

“…Pawelka and Stöckigt 1986). The present results were in line with three UV maxima and MS data (Table 2, Online 3) obtained for isolated alkaloid from Rhazya analysed by EI-HRMS (Atta-ur-Rahman et al 1991). The MS data for alkaloid 4 from other plant species (Yamazaki et al 2003;Dhooghe et al 2008;Donalisio et al 2013) have been also consistent with the present results.…”

Section: Plant Cell Repsupporting

confidence: 92%

“…Strictosidine lactam (4) is a highly polar alkaloid containing a sugar moiety (Atta-ur-Rahman et al 1991) in R. stricta and its cell cultures (e.g. Pawelka and Stöckigt 1986).…”

Section: Plant Cell Repmentioning

confidence: 99%

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Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

et al. 2015

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Transgenic hairy roots of R. stricta were developed for investigation of alkaloid accumulations. The contents of five identified alkaloids, including serpentine as a new compound, increased compared to non-transformed roots. Rhazya stricta Decne. is a rich source of pharmacologically active terpenoid indole alkaloids (TIAs). In order to study TIA production and enable metabolic engineering, we established hairy root cultures of R. stricta by co-cultivating cotyledon, hypocotyl, leaf, and shoot explants with wild-type Agrobacterium rhizogenes strain LBA 9402 and A. rhizogenes carrying the pK2WG7-gusA binary vector. Hairy roots initiated from the leaf explants 2 to 8 weeks. Transformation was confirmed by polymerase chain reaction and in case of GUS clones with GUS staining assay. Transformation efficiency was 74 and 83% for wild-type and GUS hairy root clones, respectively. Alkaloid accumulation was monitored by HPLC, and identification was achieved by UPLC-MS analysis. The influence of light (16 h photoperiod versus total darkness) and media composition (modified Gamborg B5 medium versus Woody Plant Medium) on the production of TIAs were investigated. Compared to non-transformed roots, wild-type hairy roots accumulated significantly higher amounts of five alkaloids. GUS hairy roots contained higher amounts two of alkaloids compared to non-transformed roots. Light conditions had a marked effect on the accumulation of five alkaloids whereas the composition of media only affected the accumulation of two alkaloids. By successfully establishing R. stricta hairy root clones, the potential of transgenic hairy root systems in modulating TIA production was confirmed.

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“…These data were in accordance with those reported by Atta-ur-Rahman et al (1991) except the slight maximum at 298 nm and at 305.…”

Section: Plant Cell Repsupporting

confidence: 93%

Section: Plant Cell Repsupporting

confidence: 92%

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Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

et al. 2015

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“…In the case of the bhimberine sample, one of the impurities may have been (1 6R)-E-isositsirikine acetate (vide supra), whose presence in Rhazya stricta has been claimed (8). Both original samples seem to have been contaminated by some impurities.…”

mentioning

confidence: 99%

The Rhazimanine-Bhimberine Enigma

Lounasmaa¹,

Jokela²,

Hanhinen³

et al. 1995

J. Nat. Prod.

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“…Askham and Cornelius (1970) found a negative effect of saltbush, Atriplex canescens, containing saponin, on seed germination of many subject plants at high concentrations, but interestingly, the growth was stimulated at low concentrations. Other desert shrubs such as Rhazya stricta contain alkaloides (Atta ur Rahman et al, 1991) that completely inhibited the germination of many other range plants in Saudi Arabia (Assaeed and Al-Doss, 1997). The importance of such toxic substances is greater in desert region because of restricted rainfall inhibiting leaching from soil allowing allelopathic compounds to residue for a long time (Sen and Chawan, 1970).…”

Section: Role Of Allelopathy In Treesmentioning

confidence: 99%

Interactions of allelopathy and competition affecting Ziziphus spina-christi and Prosopis juliflora seedlings

Alshahrani¹

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Interactions of allelopathy and competition affecting growth of Ziziphus spina-christi and Prosopis juliflora seedlings. Thobayet S. Alshahrani This study consists of two parts, provides data on the interference between Ziziphus spina-christi, native to Saudi Arabia, and Prosopis juliflora, an introduced species. Dry leaves from both species were milled and used to prepare extracts using 0g/l, 5g/l, 20g/l, 40g/l, 60g/l, and 100 g/l of dried leaves. Leaf extract of P. juliflora was used to irrigate Z. spina-christi with 120 ml weekly over a 7-month growth period, and vice versa. Plant height, plant diameter, number of leaves, leaf area, chlorophyll content, total root length, number of root tips, root diameter, root surface area, root volume, shoot dry weight, root dry weight, and root to shoot ratio were measured monthly. Ziziphus spinachristi leaf extract produced a negative impact on all studied parameters of P. juliflora and the negative impact increased with increasing extract concentrations. Lower leaf concentration extracts of P. juliflora positively affected the studied parameters of Z. spinchristi. At 60 g/l the growth of Z. spina-christ was highly stimulated but at 100 g/l all the growth parameters were reduced. The study explored the negative effect of Z. spinachristi leaf extracts on the growth of other plant species where no such study has been conducted before. The second part is a study of the effect of interaction between interspesific competition and nitrogen levels on growth of Prosopis juliflora and Ziziphus spinachristi seedlings for 7-months. Dry weight, root:shoot ratio, leaf area, plant height, plant diameter, total chlorophyll, relative yield, and aggressivity were measured and/or calculated. In mixed plantings with a high level of nitrogen, Prosopis juliflora exceeded the growth of the native species in most studied parameters but the effect of intraspesific competition was high. Under a low level of nitrogen the native species growth exceeded that of P. juliflora. In monoculture plantings, both species showed a reduction in growth parameters with increasing numbers of plants per pot and the reduction in low levels of nitrogen exceeded that in high levels of nitrogen. For total dry weight and leaf area, seedlings of Z. spina-christi grown in low levels of nitrogen exceeded that of seedlings grown in high levels of nitrogen. iii DEDICATION To my wife To my children Omar Tamador Norah Mohaned iv ACKNOWLEDGEMENT In the beginning, all my duty, appreciation and thankfulness goes to Allah (God) for his mercy, help, and guidance to the right way and without his help nothing of this work can exist. With sincere appreciation, I would like to thank my advisor Dr. Ray Hicks Jr. for guidance, support, understanding and helpful comments and criticisms. His guidance advice and patience make a lot of things easier. To my committee members, Drs. Sven Verlinden, Devinder K. Bhumbla, Richard B. Thomas, and Jonathan R. Cumming, I would like to thank for helpful comments, encouragement and suggestions during ...

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Alkaloids from Rhazya stricta

Cited by 46 publications

References 39 publications

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

The Rhazimanine-Bhimberine Enigma

Interactions of allelopathy and competition affecting Ziziphus spina-christi and Prosopis juliflora seedlings

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