Alkaloids from Chelidonium majus and their inhibitory effects on LPS-induced NO production in RAW264.7 cells

Park, Ji Eun; Cuong, To Dao; Hung, Tran Manh; Lee, Ik‐Soo; Na, MinKyun; Kim, Jin Cheol; Ryoo, Sungwoo; Lee, Jeong Hyung; Choi, Jae Sue; Woo, Mi Hee; Min, Byung Sun

doi:10.1016/j.bmcl.2011.09.128

Cited by 74 publications

(40 citation statements)

References 16 publications

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“…This and previous studies found that baicalin, one of the most enriched compounds in BSE, was effective in the inhibition of NO and PGE 2 productions induced by LPS [35]. On the other hand, although berberine (10 μ M) inhibited PGE 2 accumulation, it failed to block in the NO induction, which was matched with previous findings (IC 50 > 30 μ M) [36]. Therefore, baicalin might, at least in part, be one of the active compounds in BSE in the aspects of anti-inflammation in LPS-stimulated macrophages.…”

Section: Discussionsupporting

confidence: 90%

Bojesodok-eum, a Herbal Prescription, Ameliorates Acute Inflammation in Association with the Inhibition of NF-κB-Mediated Nitric Oxide and ProInflammatory Cytokine Production

Sohn

Cho

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Bojesodok-eum (BSE) is a herbal prescription consisting of Coptidis Rhizoma and Scutellariae Radix as main components. This paper investigated the effects of BSE on the induction of nitric oxide (NO), prostaglandin E2 (PGE2), and proinflammatory cytokines that are caused by lipopolysaccharide (LPS) in murine macrophage cell line and on the paw edema formation in animals. Administration of BSE (0.3 g/kg and 1 g/kg) in rats significantly inhibited carrageenan-induced paw edema formation, as did dexamethasone, an anti-inflammatory positive control drug. In cell model, treatment of BSE decreased the production of NO and PGE2 in RAW264.7 cells stimulated by LPS. BSE also inhibited the expression of iNOS and COX-2 protein as well as COX activity in a concentration-dependent manner. Consistently, BSE suppressed the ability of LPS to produce TNF-α, interleukin-1β, and interleukin-6. LPS treatment induced nuclear NF-κB level and I-κBα phosphorylation, which were inhibited subsequent treatment of BSE, suggesting its repression of LPS-inducible NF-κB activation. BSE abrogated the induction of NO, PGE2, and proinflammatory cytokines, as well as iNOS and COX-2 protein expression in RAW264.7 cells stimulated by LPS as mediated with NF-κB inhibition.

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Section: Discussionsupporting

confidence: 90%

Bojesodok-eum, a Herbal Prescription, Ameliorates Acute Inflammation in Association with the Inhibition of NF-κB-Mediated Nitric Oxide and ProInflammatory Cytokine Production

Sohn

Cho

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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“…Published data show the modest anti-proliferative potency of chelidonine (Table S2). IG50 of chelidonine varies from 2.58 µM for A375 cells to over 141 µM for A549 cells [9], [62]–[66]. Calculated IG50 of chelidonine in Am for HeLa, HT29 and A549 cells was 0.478 µM, 1.7 µM, 1.45 µM respectively.…”

Section: Discussionmentioning

confidence: 99%

Amitozyn Impairs Chromosome Segregation and Induces Apoptosis via Mitotic Checkpoint Activation

et al. 2013

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Amitozyn (Am) is a semi-synthetic drug produced by the alkylation of major celandine (Chelidonium majus L.) alkaloids with the organophosphorous compound N,N’N’-triethylenethiophosphoramide (ThioTEPA). We show here that the treatment of living cells with Am reversibly perturbs the microtubule cytoskeleton, provoking a dose-dependent cell arrest in the M phase. Am changed the dynamics of tubulin polymerization in vitro, promoted the appearance of aberrant mitotic phenotypes in HeLa cells and induced apoptosis by the activation of caspase-9, caspase-3 and PARP, without inducing DNA breaks. Am treatment of HeLa cells induced changes in the phosphorylation of the growth suppressor pRb that coincided with maximum mitotic index. The dose-dependent and reversible anti-proliferative effect of Am was observed in several transformed cell lines. Importantly, the drug was also efficient against multidrug-resistant, paclitaxel-resistant or p53-deficient cells. Our results thus open the way to further pre-clinical evaluation of Am.

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“…It was also reported that chelidonine and hydroxydihydrosanguinarine inhibit LPS-induced NO production in macrophage RAW264.7 cells. Cox-2 and iNOS mRNA level were decreased by chelidonine and hydroxydihydrosanguinarine in LPS treated RAW264.7 cells (Park et al, 2011). Chelidonine and sanguinarine caused apoptosis in human acute T-lymphoblastic leukaemia MT-4 cells (Philchenkov et al, 2008).…”

Section: Discussionmentioning

confidence: 98%

“…It contains isoquiniline alkaloids such as sanguinarine, chelidonine, chelerythrine, berberine, propopine, and coptisine (Gilca et al, 2010). CM has a various biological activities such as anti-inflammatory (Park et al, 2011), antimicrobial (Meng et al, 2009), antioxidant (Nadova et al, 2008), anticancer (Habermehl et al, 2006;Noureini and Wink, 2009;Paul et al, 2012), immunomodulatory (Chung et al, 2004), radioprotective (Song et al, 2003), and nephronprotective activities (Koriem et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Its Mechanism of Chelidonium majus Extract

Heo¹,

Kim²,

Lee³

et al. 2013

Korean Journal of Medicinal Crop Science

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: Chelidonium majus (CM) contains several isoquinoline alkaloids that have been reported to have various biological activities such as anti-inflammatory, antimicrobial, antioxidant, immune-modulatory, and antitumoral. It has been reported that the extract of CM had an antioxidant potential, however the mechanism has not been verified. In this study, we found that CM extract activated FOXO3a. FOXO3a is a transcription factor that involved in various biological processes such as cell cycle arrest, apoptosis, DNA repair, and ROS detoxification. Transcriptional activities of FOXO3a were regulated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Protein level of FOXO3a was increased by CM extract. Promoter activities of FOXO-transcriptional target genes such as MnSOD, p27 and GADD45 were activated by CM extract in a dose dependent manner. In addition, protein level of MnSOD, major antioxidant enzyme, was increased by CM extract. Thereby ROS level was decreased by CM in old HEF cells. These results suggest that CM extract has an antioxidant activity through FOXO activation.

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Alkaloids from Chelidonium majus and their inhibitory effects on LPS-induced NO production in RAW264.7 cells

Cited by 74 publications

References 16 publications

Bojesodok-eum, a Herbal Prescription, Ameliorates Acute Inflammation in Association with the Inhibition of NF-κB-Mediated Nitric Oxide and ProInflammatory Cytokine Production

Bojesodok-eum, a Herbal Prescription, Ameliorates Acute Inflammation in Association with the Inhibition of NF-κB-Mediated Nitric Oxide and ProInflammatory Cytokine Production

Amitozyn Impairs Chromosome Segregation and Induces Apoptosis via Mitotic Checkpoint Activation

Antioxidant Activity and Its Mechanism of Chelidonium majus Extract

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