Alkaloids as potential anti-tubercular agents

Kishore, N.; Mishra, Bhuwan B.; Tripathi, Vishal; Tiwari, Vinod K.

doi:10.1016/j.fitote.2009.01.002

Cited by 77 publications

(46 citation statements)

References 86 publications

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“…Flavonoids may act through inhibiting cytoplasmic membrane function as well as by inhibition of DNA gyrase and β-hydroxyacyl-acyl carrier protein dehydratase activities [19,31]. Alkaloids was reported by Kishore et al (2009), that alkaloids have been found more effective anti-tubercular against M. tuberculosis [30]. The mechanism of alkaloid compounds is by disrupting the peptidoglycan component of the bacterial cell so that the cell wall layer is not formed intact and affect the amino acids of cell wall and bacterial DNA [6,33].…”

Section: Discussionmentioning

confidence: 99%

Anti-Tubercular Activity of Extract and Coumpounds of Noni (Morinda Citrifolia Linn)

Mauliku

Hendro

Saputro

et al. 2017

Int J Pharm Pharm Sci

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Objective: The study evaluated the anti-tubercular activity of crude extracts and active compounds which was isolated, purification and characterization from noni (Morinda citrifolia Linn) against Mycobacterium tuberculosis strains H37Rv. Methods:The crude extract of the noni fruits was macerated using ethanol (96%). Ethanol crude extracts were qualitatively screened to identify of the flavonoid, scopoletin, antraquinon and alkaloids using phytochemical fractionation by harborn method. The anti-tubercular activity of noni fruit and its combination were determined by the minimum inhibitory concentration (MIC) of the bacterial growth at various doses 10 mg/ml, 20 mg/ml, 30 mg/ml, and 40 mg/ml using agar well diffusion method. Statistical analysis was performed by analysis of variance. Results:Crude extract and active compounds of noni fruit such as flavonoid, scopoletin, antraquinon and alkaloids significantly work as antitubercular against Mycobacterium tuberculosis strain H37Rv at various doses (p value=0,000). Crude extract (59.00±60.513), alkaloids (64.83±49.356), anthraquinones (69.50±50.396), and flavonoid (72.92±58.728) showed the highest anti-tubercular activity in inhibiting the growth of Mycobacterium tuberculosis strain H37Rv compared to scopoletin (95.92±33.280) and the negative control (189.25±33.280). The minimum inhibitory concentration was found at a dose of 40 mg/ml. Conclusion:Crude extract of noni fruit was the most active anti-tubercular against Mycobacterium tuberculosis strains H37Rv. One can assume that noni fruit can be fruther researched to be used as adjuvant therapy for anti-tuberculosis drugs.

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Section: Discussionmentioning

confidence: 99%

Anti-Tubercular Activity of Extract and Coumpounds of Noni (Morinda Citrifolia Linn)

Mauliku

Hendro

Saputro

et al. 2017

Int J Pharm Pharm Sci

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“…Another alkaloid, lunacridine and its trifluoroacetyl derivative were found to be effective for DNA intercalation, topoisomerase II decatenation, cytotoxicity and caspase activation activities 15 . The presence of a fully aromatic ring and 4-methoxyl group of quinoline alkaloids were attributed to be the active principle in the antitubercular activity of the extract against M. tuberculosis multidrug resistant strains 40,22 .…”

Section: Fig 3: Percent Cell Proliferation Of Bal B/c Mice Splenocytmentioning

confidence: 99%

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2018

IJPSR

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ABSTRACT:This study was conducted to evaluate the antimicrobial and cellular metabolic inhibitory properties of the ethanolic extract from the bark of 'Lunas-bagon', Lunasia sp. Extracts were evaluated using the agar well diffusion method for antimicrobial assay. The standard phytochemical screening was done for the determination of compounds that can be considered toxic and MTT assay for the effects of the extract on cellular metabolism. Results showed that the extracts have ability to inhibit selected Gram-positive and Gram-negative bacterial isolates indicating that the extracts are effective antibacterials. Results from the MTT assay revealed at doses lower than 100.0 ug/ml, extracts will not inhibit cellular metabolism of the splenocytes. Higher dose, however shows reduction of metabolic activity which may indicate the onset of apoptosis in cells. Basic biochemical tests indicated the absence of cyanogenic glycosides and anthraquinones, thus the extracts may not have toxic effects. Identification of bioactive compounds in through GC-MS analysis showed the presence of compounds that are known as antimicrobials and also effective in the treatment of selected healthrelated problems and conditions. The information generated in this study clearly indicates that the folk medical uses of 'Lunas-bagon', Lunasia sp. have antimicrobial and biochemical bases.

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“…Different types of nitrogen-containing substances have been found to display antimicrobial [119,120], antifungal, antiparasitary [121] and antiviral [122] effects. Chinese traditional phytomedicine is a vast source of bioactive scaffolds, and berberine 6 is one interesting representative, originally isolated from Coptis chinensis rhizome [123].…”

Section: Alkaloidsmentioning

confidence: 99%