Alkaliphilic, Alkalitolerant Microorganisms

Kanekar, Pradnya Pralhad; Kanekar, Sagar Pralhad

doi:10.1007/978-981-19-1573-4_3

Cited by 3 publications

(2 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adding to its distinctiveness, the lake is divided into two distinct parts, each with different chemical compositions. The outer region maintains a neutral pH of 7, while the inner area is highly alkaline, registering a pH of 11 (Kanekar and Kanekar, 2022). Each of these areas hosts unique ora and fauna, contributing to the lake's exceptional ecological diversity.…”

Section: Resultsmentioning

confidence: 99%

The Ecological Tapestry of Lonar Crater: Biodiversity, Conservation Challenges, and Preservation Strategies of the Ecosystem

Dighe,

Singh,

Ragde

et al. 2024

Preprint

View full text Add to dashboard Cite

The Lonar Crater, a geological marvel located in Buldana District of India’s Maharashtra state, stands as a unique testament to Earth's natural history, formed around 30,000-50,000 years ago by a meteorite impact on Deccan Plateau basalts. The floral and faunal study of Lonar Lake is a comprehensive research endeavor aimed at understanding the biodiversity and ecological dynamics of this unique ecosystem. The study covered various significant points around the lake spanning a total distance of 3.2 km (aerial) and then back to the Lonar Lake entry point. The main objective of this study was to investigate the occurrence of native flora and fauna and to assess the impact of introduced species on the native biodiversity of the area. This study deals into the diverse flora and fauna of the Lonar ecosystem, revealing 85 plant species, including natives, naturalized, and introduced varieties, alongside a rich avian and reptilian fauna. However, presently the ecosystem faces challenges, including invasive species, deforestation, and pollution. This research underscores the urgency of conservation efforts to protect Lonar's ecological balance and preserve its unique biodiversity.

show abstract

Section: Resultsmentioning

confidence: 99%

The Ecological Tapestry of Lonar Crater: Biodiversity, Conservation Challenges, and Preservation Strategies of the Ecosystem

Dighe,

Singh,

Ragde

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Bacteria, which constituted the majority of the microorganisms (either isolated or from the collection) capable of growing in NEM, are generally known to grow in an optimum pH near neutral (6-7.5). However, some bacteria can tolerate or even require alkaline conditions (pH > 9) due to their evolutionary adaptation to extreme environmental conditions, allowing them to colonize various habitats [60,61]. On the other hand, the growth of two fungi and two yeasts in NEM is noteworthy, considering that these microorganisms typically prefer acidic environments (pH < 6).…”

Section: Isolation and Selection Of The Microorganismmentioning

confidence: 99%

Bioremediation with an Alkali-Tolerant Yeast of Wastewater (Nejayote) Derived from the Nixtamalization of Maize

Román-Escobedo,

Cristiani-Urbina,

Morales-Barrera

2024

Fermentation

View full text Add to dashboard Cite

Nejayote, the wastewater from the nixtamalization of maize, is difficult to biodegrade due to its abundant calcium content; low levels of nitrogen, phosphorus, and easily assimilable sugars; elevated pH; and high chemical oxygen demand (COD). The aim of the present study was to isolate microorganisms capable of utilizing filtered nejayote (NEM) as the only source of carbon for growth and to test the best microorganism for the bioremediation of this wastewater by lowering the level of pH and COD. Of the 15 strains of microorganisms tested, Rhodotorula mucilaginosa LCRE was chosen and identified using molecular techniques. Subsequently, its growth kinetics were characterized during cultivation in unenriched NEM (control) and NEM enriched with nitrogen and phosphorus salts. R. mucilaginosa LCRE showed a greater growth (6.9 ≤ X ≤ 8.9 g L−1), biomass yield (0.33 ≤ YX/S ≤ 0.39 g g−1), and specific growth rate (0.748 ≤ µ ≤ 0.80 day−1) in the enriched versus control NEM (X = 6.55 g L−1, YX/S = 0.28 g g−1, and µ = 0.59 day−1). However, a higher total sugar consumption (94.98%), better COD removal efficiency (75.5%), and greater overall COD removal rate (1.73 g L−1 h−1) were found in the control NEM. Hence, R. mucilaginosa LCRE holds promise for the efficient bioremediation of nejayote without costly pretreatments or nutrient supplementation.

show abstract

Exogenous putrescine plays a switch-like influence on the pH stress adaptability of biofilm-based activated sludge

Jiang,

Wang,

Wang

et al. 2024

Appl Environ Microbiol

View full text Add to dashboard Cite

Microbial community adaptability to pH stress plays a crucial role in biofilm formation. This study aims to investigate the regulatory mechanisms of exogenous putrescine on pH stress, as well as enhance understanding and application for the technical measures and molecular mechanisms of biofilm regulation. Findings demonstrated that exogenous putrescine acted as a switch-like distributor affecting microorganism pH stress, thus promoting biofilm formation under acid conditions while inhibiting it under alkaline conditions. As pH decreases, the protonation degree of putrescine increases, making putrescine more readily adsorbed. Protonated exogenous putrescine could increase cell membrane permeability, facilitating its entry into the cell. Subsequently, putrescine consumed intracellular H + by enhancing the glutamate-based acid resistance strategy and the γ-aminobutyric acid metabolic pathway to reduce acid stress on cells. Furthermore, putrescine stimulated ATPase expression, allowing for better utilization of energy in H + transmembrane transport and enhancing oxidative phosphorylation activity. However, putrescine protonation was limited under alkaline conditions, and the intracellular H + consumption further exacerbated alkali stress and inhibits cellular metabolic activity. Exogenous putrescine promoted the proportion of fungi and acidophilic bacteria under acidic stress and alkaliphilic bacteria under alkali stress while having a limited impact on fungi in alkaline biofilms. Increasing Bdellovibrio under alkali conditions with putrescine further aggravated the biofilm decomposition. This research shed light on the unclear relationship between exogenous putrescine, environmental pH, and pH stress adaptability of biofilm. By judiciously employing putrescine, biofilm formation could be controlled to meet the needs of engineering applications with different characteristics. IMPORTANCE The objective of this study is to unravel the regulatory mechanism by which exogenous putrescine influences biofilm pH stress adaptability and understand the role of environmental pH in this intricate process. Our findings revealed that exogenous putrescine functioned as a switch-like distributor affecting the pH stress adaptability of biofilm-based activated sludge, which promoted energy utilization for growth and reproduction processes under acidic conditions while limiting biofilm development to conserve energy under alkaline conditions. This study not only clarified the previously ambiguous relationship between exogenous putrescine, environmental pH, and biofilm pH stress adaptability but also offered fresh insights into enhancing biofilm stability within extreme environments. Through the modulation of energy utilization, exerting control over biofilm growth and achieving more effective engineering goals could be possible.

show abstract

Alkaliphilic, Alkalitolerant Microorganisms

Cited by 3 publications

References 118 publications

The Ecological Tapestry of Lonar Crater: Biodiversity, Conservation Challenges, and Preservation Strategies of the Ecosystem

The Ecological Tapestry of Lonar Crater: Biodiversity, Conservation Challenges, and Preservation Strategies of the Ecosystem

Bioremediation with an Alkali-Tolerant Yeast of Wastewater (Nejayote) Derived from the Nixtamalization of Maize

Exogenous putrescine plays a switch-like influence on the pH stress adaptability of biofilm-based activated sludge

Contact Info

Product

Resources

About