Alkaline xylanases from Bacillus mojavensis A21: Production and generation of xylooligosaccharides

Haddar, Anissa; Driss, Dorra; Frikha, Fakher; Ellouz-Chaabouni, Semia

doi:10.1016/j.ijbiomac.2012.06.036

Cited by 39 publications

(23 citation statements)

References 49 publications

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“…This is in accordance with the results of the xylanases from B. mojavensis A21 (Haddar et al 2012), G. mesophila KMM241 (Guo et al 2013), Geobacillus thermoleovorans (Verma and Satyanarayana 2012), M. cinnamomea (Fan et al 2014), etc. While some other xylanases showed xylosidase activity, the end product was xylose, such as originated from Thermoascus thermarum (Shi et al 2013), Thermoascus aurantiacus (Zhang et al 2011), the rumen contents of Hu sheep, and Caldicoprobacter algeriensis (Amel et al 2016).…”

Section: Substrate Specificity and Analysis Of Hydrolytic Productssupporting

confidence: 91%

“…These results indicated that Xyn10A was stable in moderate and slightly alkaline conditions. This was similar to the xylanases from Bacillus mojavensis A21 (Haddar et al 2012), as its best activity was also found at pH 8.0. Moreover, several alkaliphilic xylanases from the same genus (Bacillus) showed an optimum activity at pH 9.0 to 10.0, such as B. pumilus SV-85S (Nagar et al 2010), and B. halodurans MTCC 9512 and S7 (Mamo et al 2006;Mamo et al 2009;Garg et al 2009).…”

Section: The Properties Of Xyn10a Effect Of Ph and Temperature On Actsupporting

confidence: 74%

“…Xylanases originating from the Bacillus strains are intrinsically interesting because of their characteristics, such as alkali and salt tolerance, cold adaptation, or thermostability; they are widely employed in industrial applications (Chang et al 2004;Huang et al 2006;Mamo et al 2006;Ruller et al 2008;Khandeparker et al 2011;Haddar et al 2012;Thomas et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Sun

Shi

et al. 2016

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A xylanase (Xyn10A) gene from the saline-alkali-tolerant microorganism Bacillus cellulosilyticus DSM 2522 was cloned and expressed in Escherichia coli BL21 (DE3). The open reading frame was composed of 1008 base pairs, and it encoded 335 amino acid residues belonging to glycosyl hydrolase family 10. The optimal temperature and pH of the purified Xyn10A were 40 °C and 8.0, respectively. The Xyn10A was sensitive to heat and showed obvious cold-adapted activity, retaining 38.3%, 55.7%, and 82.9% of the optimal activity at 4, 20, and 30 °C, respectively. Xyn10A also showed a high level of NaCl tolerance. The highest activity was observed with 1.5 M NaCl. The specific enzyme activity of Xyn10A was as much as 163.8 U/mg. Kinetic assays showed that Km, Vmax, and Kcat were 2.56 mg/mL, 202.5 μM/min/mg, and 132.6 /s, respectively. Additionally, the main hydrolysis products using birchwood xylan as substrate were xylobiose, xylotriose, and xylotetraose, as determined by thin layer chromatography analysis. As a cold-adapted and salt-tolerant enzyme, Xyn10A is an ideal candidate for further research and biotechnological applications.

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Section: Substrate Specificity and Analysis Of Hydrolytic Productssupporting

confidence: 91%

Section: The Properties Of Xyn10a Effect Of Ph and Temperature On Actsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Sun

Shi

et al. 2016

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“…This heteropolysaccharide is composed of several sugars, including glucose, arabinose, mannose, galactose, and galacturonic acid, branched in the main chain of xylan, composed of xylose monomers linked through β-1,4-glycosidic bonds (ALVES- PRADO et al, 2010;HADDAR et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Xylanases are enzymes responsible for hydrolyzing the β-1,4-xylan bonds to release xylose oligosaccharides, thus reducing the degree of polymerization of the main hemicellulose chain and disrupting the plant cell wall (HADDAR et al, 2012;GOWDHAMAN et al, 2014). This catalytic property enables these enzymes to be used in the hydrolysis of vegetal biomass for the production of biofuels, the formulation of animal feed, or even in bread-making processes to improve the quality of dough, hydrolyzing xylooligosaccharides that are disadvantageous to the formation of gluten and impairs the crumb structure (BECKER et al, 2009;BRIENZO et al, 2012;OLIVEIRA et al, 2014;PIROTA et al, 2015;TERRASAN and CARMONA, 2015).…”

Section: Introductionmentioning

confidence: 99%

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Costa¹,

Scalabrini²,

Silvestre³

et al. 2016

Biosci. J.

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ABSTRACT:Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g -1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes.

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Alkaline Active Hemicellulases

Mamo¹

2019

Advances in Biochemical Engineering/Biotechnology

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Alkaline xylanases from Bacillus mojavensis A21: Production and generation of xylooligosaccharides

Cited by 39 publications

References 49 publications

Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Alkaline Active Hemicellulases

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