Alkaline semen diluent combined with R848 for separation and enrichment of dairy goat X-sperm

Huang, Min; Cao, Xinyou; He, Qifu; Yang, H.W.; Chen, Y.Z.; Zhao, J.L.; Ma, Haofeng; Kang, Jian; Liu, J.; Quang, F.S.

doi:10.3168/jds.2022-22115

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…At a sperm diluent pH of 7.4, the proportion of upper layer X-chromosome spermatozoa was 30.45% ± 1.03, which differed significantly from a proportion of 52.35% ± 1.72 in the control group (pH 6.8) [ 127 ]. A higher proportion of X-chromosome spermatozoa (85.57% ± 3.27) was observed in the alkaline diluent (pH 7.4) upon the co-administration of resiquimod [ 128 ]. pH affects mitochondrial activity and glucose uptake capacity primarily through phosphorylation of NFκB and GSK3α/β.…”

Section: Ph and Regulation Of Sperm Functionmentioning

confidence: 99%

pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization

Dai,

Zou,

Cai

et al. 2024

Biomolecules

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pH homeostasis is crucial for spermatogenesis, sperm maturation, sperm physiological function, and fertilization in mammals. HCO3− and H+ are the most significant factors involved in regulating pH homeostasis in the male reproductive system. Multiple pH-regulating transporters and ion channels localize in the testis, epididymis, and spermatozoa, such as HCO3− transporters (solute carrier family 4 and solute carrier family 26 transporters), carbonic anhydrases, and H+-transport channels and enzymes (e.g., Na+-H+ exchangers, monocarboxylate transporters, H+-ATPases, and voltage-gated proton channels). Hormone-mediated signals impose an influence on the production of some HCO3− or H+ transporters, such as NBCe1, SLC4A2, MCT4, etc. Additionally, ion channels including sperm-specific cationic channels for Ca2+ (CatSper) and K+ (SLO3) are directly or indirectly regulated by pH, exerting specific actions on spermatozoa. The slightly alkaline testicular pH is conducive to spermatogenesis, whereas the epididymis’s low HCO3− concentration and acidic lumen are favorable for sperm maturation and storage. Spermatozoa pH increases substantially after being fused with seminal fluid to enhance motility. In the female reproductive tract, sperm are subjected to increasing concentrations of HCO3− in the uterine and fallopian tube, causing a rise in the intracellular pH (pHi) of spermatozoa, leading to hyperpolarization of sperm plasma membranes, capacitation, hyperactivation, acrosome reaction, and ultimately fertilization. The physiological regulation initiated by SLC26A3, SLC26A8, NHA1, sNHE, and CFTR localized in sperm is proven for certain to be involved in male fertility. This review intends to present the key factors and characteristics of pHi regulation in the testes, efferent duct, epididymis, seminal fluid, and female reproductive tract, as well as the associated mechanisms during the sperm journey to fertilization, proposing insights into outstanding subjects and future research trends.

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Section: Ph and Regulation Of Sperm Functionmentioning

confidence: 99%

pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization

Dai,

Zou,

Cai

et al. 2024

Biomolecules

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“…Microbial biofilms are multicellular communities wherein bacterial cells are surrounded by extracellular polymeric substances (EPS), including a variety of environmental DNA (eDNA), polysaccharides, proteins and phospholipids (Yuan et al 2023a). This mode of life might be another survival strategy for B. cereus in dairy processing environments.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and risk‐related features of Bacillus cereus in fluid milk

Dai,

Yuan,

Fan

et al. 2024

Int J of Dairy Tech

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This work assessed the prevalence and risk‐related phenotypes of Bacillus cereus in milk products. Bacillus was the most dominant genus of 113 collected strains from 41 milk samples, and B. cereus (19.27%) was the most abundant species showing a high genetic diversity with seven random amplified polymorphic DNA patterns. Most B. cereus isolates were strong biofilm formers with good motility, extracellular polymeric substance‐producing ability, high proteolytic and lipolytic activities. Antibiotic resistance analysis suggested the majority B. cereus strains were resistant to oxacillin (90.48%), tetracycline (95.24%), and penicillin G sodium (47.62%). Results revealed the genotypic diversity of B. cereus, and their potential risk in dairy industry.

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“…Their research elucidated that R848 treatment inhibited X sperm motility in goats through the TLR7/8 signaling pathway, affecting X sperm ATP levels via the GSK3α/β–hexokinase pathway. In 2022, Huang [ 20 ] combined R848 with an alkaline semen diluent, achieving the successful isolation and enrichment of goat X sperm. This sex control technique, relying on the R848 activation of X sperm TLR7/8, was successfully validated in several mammals, including mice, cattle, and sheep, confirming the robustness of this sex control technology [ 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…In 2022, Huang [ 20 ] combined R848 with an alkaline semen diluent, achieving the successful isolation and enrichment of goat X sperm. This sex control technique, relying on the R848 activation of X sperm TLR7/8, was successfully validated in several mammals, including mice, cattle, and sheep, confirming the robustness of this sex control technology [ 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Gender Control of Mouse Embryos by Activation of TLR7/8 on X Sperm via Ligands dsRNA-40 and dsRNA-DR

Hou,

Peng,

Hong

et al. 2024

Molecules

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Gender control technologies are promising for enhancing the production efficiency of the farm animal industry, and preventing sex-linked hereditary diseases in humans. It has been shown that the X sperm of mammalian animals specifically expresses X-chromosome-derived toll-like receptor 7/8 (TLR7/8), and the activation of TLR7/8 on the X sperm by their agonist, R848, can separate X and Y sperm via the specific inhibition of X sperm motility. The use of R848-preselected sperm for fertilization resulted in sex-ratio-skewed embryos or offspring. In this study, we aimed to investigate whether two other TLR7/8 ligands, double-stranded RNA-40 (dsRNA-40) and double-stranded RNA-DR (dsRNA-DR), are also effective in the separation of mouse X and Y sperm and the subsequent generation of gender-ratio-skewed in vitro fertilization (IVF) embryos. Our results indicated that cholesterol modification significantly enhances the transfection of dsRNA-40 and dsRNA-DR into sperm cells. dsRNA-40 and dsRNA-DR incubation with mouse sperm could separate X and Y sperm by the specific suppression of X sperm motility by decreasing its ATP level and mitochondrial activity. The use of a dsRNA-40- or dsRNA-DR-preselected upper layer of sperm, which predominantly contains high-motility Y sperm, for IVF caused a male-biased sex ratio shift in resulting embryos (with 65.90–74.93% of embryos being male). This study develops a simple new method for the efficient separation of mammalian X and Y sperm, enabling the selective production of male or female progenies.

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Alkaline semen diluent combined with R848 for separation and enrichment of dairy goat X-sperm

Cited by 8 publications

References 45 publications

pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization

pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization

Occurrence and risk‐related features of Bacillus cereus in fluid milk

Gender Control of Mouse Embryos by Activation of TLR7/8 on X Sperm via Ligands dsRNA-40 and dsRNA-DR

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