Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis

Agrawal, Deepti; Patidar, Pankaj; Banerjee, Tushar; Patil, Sandeep

doi:10.1016/j.procbio.2004.03.006

Cited by 53 publications

(32 citation statements)

References 24 publications

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“…The medium was inoculated with fungal hyphae discs and incubated at 28±2°C. After 5 days, the radial growth of colonies and substrate clearing zones were measured (Agrawal et al, 2005).…”

Section: Screening Of Protease Production By Plate Agarmentioning

confidence: 99%

“…The strain was maintained on potato dextrose agar slants at 4°C and subculture for each two months on the same medium to preserve its vitality. The inoculum for fermentation process was prepared by scarping off the spores from cultures grown on potato dextrose agar (28±2°C for 5-7 days) in a sterile solution of 0.1% Tween-80 (10ml/ Petri plate) under aseptic conditions (Agrawal et al, 2005;Sandhya et al, 2005). After filtration through Whatman paper no.1, the absorbance of the suspension was measured at 650nm (Markwell et al, 1989).…”

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confidence: 99%

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Optimization of Milk-Clotting Protease Production by a Local Isolate of Aspergillus Niger Ffb1 in Solid-State Fermentation

Bensmail¹,

Mechakra²,

Fazouane–Naimi³

2015

J microb biotech food sci

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The need to surmount the limitation of obtaining rennin, has been actively pushed researches to find new substitutes that present high milk-clotting activity which enables the production of high yields of cheese. In this study, the production of extracellular milk-clotting protease by locally isolated fungal specie, Aspergillus niger FFB1 under solid-state fermentation (SSF) using cheep agro-industrial byproduct (wheat bran) was optimized. The effects of several physicochemical and environmental factors were investigated to select the optimal conditions that ensure the best milk-clotting activity by application of "One-factor-at-a-time" method. A trial of cheese production using the crude extract was also carried out. The maximum enzyme activity (830 SU/g bran with a ratio MCA/PA of 4.25) was obtained under the optimum conditions of temperature (30°C), spores concentration (106 spores/mL), incubation time (72 hours), and moisture content of solid substrate (39.2%) adjusted suitably with mineral solution (Czapek-Dox) of pH 4.

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“…The medium was inoculated with fungal hyphae discs and incubated at 28±2°C. After 5 days, the radial growth of colonies and substrate clearing zones were measured (Agrawal et al, 2005).…”

Section: Screening Of Protease Production By Plate Agarmentioning

confidence: 99%

mentioning

confidence: 99%

Optimization of Milk-Clotting Protease Production by a Local Isolate of Aspergillus Niger Ffb1 in Solid-State Fermentation

Bensmail¹,

Mechakra²,

Fazouane–Naimi³

2015

J microb biotech food sci

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“…Our results are in agreement with the observation of Castilho et al (2000), who also found that acetate buffer (pH 4.4) extracted pectinases from wheat bran by Aspergillus niger better than glycerol or distilled water. Adsorption of the enzyme to cells or solid substrates has been attributed to ionic bond, hydrogen bond and Van der Waal's forces (Agrawal et al, 2005). Extraction increased with buffer as compared to distilled water probably due to the salting-in effect of the salt.…”

Section: Effect Of Solvent Type On Enzyme Extractionmentioning

confidence: 99%

Utilization of orange bagasse and molokhia stalk for production of pectinase enzyme

Ahmed

Mostafa

2013

Braz. J. Chem. Eng.

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-Studies were conducted on the production and extraction of exo-polygalacturonase (PG) in solidstate fermentation (SSF) using orange bagasse (Ob) and molokhia stalks (Ms) as a new solid support by Penicillium pinophlilum Hedg 3503 NRRL. The parameters affecting PG production under SSF were optimized. The maximum PG activity of ~ 3270 U/ g dry solid substrate was obtained from P. pinophlilum Hedg 3503 NRRL grown for 7 days on Ob and Ms in the ratio 1:3 (w/w), moistened with distilled water at 68.2% initial moisture content. Highest enzyme titers occurred in SSF without added nutrients, indicating nutrient sufficiency of the Ob and MS mixture to sustain growth and a high level of pectic substances which induced PG production. The extraction of PG from the fermented biomass was optimized. Among the various solvents tested, the maximum level of enzyme activity was achieved when acetate buffer (0.05 M; pH 5.0) was used. The optimum volume of buffer was 50 mL, an extraction time of 60 min was sufficient to extract most enzyme, which yielded 3269.6 U/ g dry solid substrate of enzyme activity. Repeated washes under the optimum conditions showed that most of the enzyme (about 98%) was recovered in three repeated extractions. Moreover, 68.9% of total activity was achieved in the first wash.

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“…[1], Serratia marcescens [52], Streptomyces sp. [8], Aspergillus clavatus [51], Beauveria feline [1], Rhizopus oryzae [3], Pseudomonas [13,45]; but from the point of view of both their historical interest and their high levels of production, the members of the genus Bacillus have been predominant [18,23,27,30,36,46,50].…”

Section: Introductionmentioning

confidence: 99%

Proteases production by two Vibrio species on residuals marine media

Vázquez¹,

Docasal²,

Mirón³

et al. 2006

J IND MICROBIOL BIOTECHNOL

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Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis

Cited by 53 publications

References 24 publications

Optimization of Milk-Clotting Protease Production by a Local Isolate of Aspergillus Niger Ffb1 in Solid-State Fermentation

Optimization of Milk-Clotting Protease Production by a Local Isolate of Aspergillus Niger Ffb1 in Solid-State Fermentation

Utilization of orange bagasse and molokhia stalk for production of pectinase enzyme

Proteases production by two Vibrio species on residuals marine media

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