Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture

Manjusha, K.; Puthumana, Jayesh; Jose, Divya; Sreelakshmi, B.; Priyaja, P.; Gopinath, Pramod; Saramma, A. V.; Singh, I. S. Bright

doi:10.1007/s10616-012-9472-z

Cited by 6 publications

(2 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We then performed a dendrogram analysis using MALDI BioTyper Compass Explorer ver.4.1 software ( Figure 5 ). Accurate distinction was feasible for most species of Vibrio including V. mimicus , V. cholerae and V. albensis , which were previously reported indistinguishable species ( Manjusha et al, 2013 ). Only a few Vibrio species could not be clearly distinguished.…”

Section: Resultsmentioning

confidence: 91%

PVBase: A MALDI-TOF MS Database for Fast Identification and Characterization of Potentially Pathogenic Vibrio Species From Multiple Regions of China

Liu

Kang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

The potentially pathogenic species of the genus Vibrio pose a threat to both humans and animals, creating medical burdens and economic losses to the mariculture industry. Improvements in surveillance and diagnosis are needed to successfully manage vibriosis outbreaks. Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can provide rapid diagnosis and has been widely used in the identification of Vibrio spp. The main weakness of this technology is the limited number of strains and species of Vibrio in the existing commercial database. Here, we develop a new in-house database named PVBase containing 790 main spectra projections (MSP) of ten Vibrio species that come from various regions of China and include abundant clinical and environmental strains. PVBase was validated through a blind test of 65 Vibrio strains. The identification accuracy and scoring of Vibrio strains was greatly improved through the addition of PVBase. Identification accuracy increased from 73.4 to 100%. The number of strains with identification scores above 2.2 increased from 53.1% to 96.9% and 53.1% of strains had an identification score above 2.59. Moreover, perfect discrimination was obtained when using all of the MSPs created for the Vibrio species, even for very closely related species such as V. cholerae, V. albensis, and V. mimicus or V. alginolyticus, V. parahaemolyticus, and V. harveyi. In addition, we used phyloproteomic analysis to study whether there are differences in protein fingerprints of different regions or pathogenic strains. We found that MSP characteristics of Vibrio species were not related to their region or source. With the construction of PVBase, the identification efficiency of potentially pathogenic Vibrio species has been greatly improved, which is an important advance for epidemic prevention and control, and aquaculture disease detection.

show abstract

Section: Resultsmentioning

confidence: 91%

PVBase: A MALDI-TOF MS Database for Fast Identification and Characterization of Potentially Pathogenic Vibrio Species From Multiple Regions of China

Liu

Kang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…(Bhargavi and Prakasham 2013 ), and Vibrio sp. (Manjusha et al 2013 ), among many other microbial strains .…”

Section: Sv1mentioning

confidence: 99%

Alkaline Proteases from Patagonian Bacteria

Olivera

Iglesias

Sequeiros

2016

Biology and Biotechnology of Patagonian Microorganisms

View full text Add to dashboard Cite

In addition to their ecological importance in the acquisition of nitrogenrich organic compounds, extracellular proteases also have interesting biotechnological applications. Particularly, alkaline proteases represent one of the most important groups of commercial enzymes. First, we introduce the classifi cation and catalytic mechanisms of proteases. Then, this chapter reviews the advances in the bioprospection of alkaline proteases produced by bacteria adapted to selective conditions from different environments of Patagonia (Argentina). Among them, the arid soils of the Patagonian Monte are propitious for the development of alkaliphilic microorganisms. Thus, we focus on the description of the species Bacillus patagoniensis and the biochemical and catalytic properties of its alkaline protease. Then, we discuss investigations about alkaline protease-producing bacteria from the southern Patagonian coast, the prevalence of psychrophilic and psychrotolerant strains, and the response of their extracellular proteases to temperature.

show abstract

Collagen and collagenolytic proteases: A review

Bhagwat

Dandge

2018

Biocatalysis and Agricultural Biotechnology

View full text Add to dashboard Cite

Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture

Cited by 6 publications

References 59 publications

PVBase: A MALDI-TOF MS Database for Fast Identification and Characterization of Potentially Pathogenic Vibrio Species From Multiple Regions of China

PVBase: A MALDI-TOF MS Database for Fast Identification and Characterization of Potentially Pathogenic Vibrio Species From Multiple Regions of China

Alkaline Proteases from Patagonian Bacteria

Collagen and collagenolytic proteases: A review

Contact Info

Product

Resources

About