Alkaline phosphatase-positive cells isolated from human hearts have mesenchymal stem cell characteristics

Abstract

Tissue-specific resident cells have been identified as a promising population of progenitor cells for cell-based therapies. We describe here the isolation from adult human hearts of tissue nonspecific alkaline phosphatase-positive cells (ALPL+ cells) with mesenchymal stem cell (MSC) characteristics. Samples from 24 adult cadaveric donors were obtained from a valve bank. Mean total ischemia time was 21.5 ± 9.1 hours. The success rate for the isolation of human heart-derived cells by the explant culture technique was 70% for the right auricle (14 of 20 trials) and 33% for the right ventricle (7 of 21 trials). The total auricle-derived cell population (TAD) was used for the purification of ALPL+ cells. TAD and ALPL+ cells expressed markers for MSC and pericytes. TAD cells and ALPL+ cells differentiated into adipocytes, osteoblasts and chondroblasts, and ALPL+ cells expressed markers of these three lineages more strongly than TAD cells, as shown by RT-PCR. This population therefore has a greater potential for differentiation into mesechymal lineages than TAD cells. Both cell populations express some markers of cardiac progenitors, such as GATA4, CD117 and VEGF. ALPL+ cells expressed troponin T and ABCG2, which are also markers of the cardiac lineage. Heart samples from tissue banks could be considered as sources of MSC with putative commitment towards cardiac lineages, even after prolonged ischemia times

Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study

“…One of the prominent properties of stem cells is their strong ability to exclude Hoechst 33342 dye, which is mediated by ABCG2 protein (Zhou et al, 2001). In fact, it has been reported that ALP (+) cells isolated from the human heart have high ABCG2 activity (de Aguiar et al, 2011). Unfortunately, the present Hoechst 33342 exclusion assay revealed that the ALP (+) HeLa cells were brightly stained, as were the ALP (-) H-1 cells (see Fig.…”

The role of dentin matrix protein 1 (DMP1) in regulation of osteogenic differentiation of rat dental follicle stem cells (DFSCs)

“…For example, mesenchymal stem cells (MSCs) could be isolated by selection of ALP-positive cells from human hearts [33]. Present study showed that DFSCs express significantly higher levels of ALP than their counterpart non-stem cells, suggesting that ALP could be a marker for identification and purification of stem cells from dental follicle.…”

Human Heart Explant-Derived Extracellular Vesicles: Characterization and Effects on the In Vitro Recellularization of Decellularized Heart Valves

“…A wide variety of cell types, such as cardiomyocytes, endothelial cells, smooth muscle cells and cardiac resident stromal cells (CRSCs), such as fibroblasts and stem/progenitor cells, are found in the adult heart [1,2]. CRSCs have been isolated and characterized by the ex vivo culture of cardiac explants [3,4,5]. The conditioned media from cultured CRSCs exhibits cardioprotective and regenerative capabilities, being able to drive proliferation, cardiac differentiation and tube formation in endothelial cells [4].…”