Alkaline phosphatase fusions in the study of cell division genes

Barondess, James J.; Carson, M.; Verduzco, L.M. Guzman; Beckwith, Jon

doi:10.1016/0923-2508(91)90044-b

Cited by 7 publications

(6 citation statements)

References 10 publications

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“…Several classes of Kanr, active AP fusions were obtained on this plasmid, including fusions to theftsI gene. We further characterized a class of fusions that mapped 5' to ftsI (2). DNA sequencing revealed that in two fusions of this class, L81 and L88 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The presence of the ftsI gene was confirmed by complementation of the ftsI(Ts) allele of strain JJ143 at the nonpermissive temperature. Plasmids pZ26::TnphoAL81, pZ26::TnphoAL88, and pZ26::TnphoAL112 were obtained by transposition of ATnphoA into plasmid pZ26 as previously described (2,20). The insertions were defined by restriction mapping, and the fusion junctions were determined by sequencing (see Fig.…”

Section: Methodsmentioning

confidence: 99%

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FtsL, an Essential Cytoplasmic Membrane Protein Involved in Cell Division in Escherichia coli

1992

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We have identified a gene involved in bacterial cell division, located immediately upstream of the ftsI gene in the min 2 region of the Escherichia coli chromosome. This gene, which we named ftsL , was detected through characterization of Tn phoA insertions in a plasmid containing this chromosomal region. Tn phoA topological analysis and fractionation of alkaline phosphatase fusion proteins indicated that the ftsL gene product is a 13.6-kDa cytoplasmic membrane protein with a cytoplasmic amino terminus, a single membrane-spanning segment, and a periplasmic carboxy terminus. The ftsL gene is essential for cell growth and division. A null mutation in ftsL resulted in inhibition of cell division, formation of long, nonseptate filaments, ultimate cessation of growth, and lysis. Under certain growth conditions, depletion of FtsL or expression of the largest ftsL-phoA fusion produced a variety of cell morphologies, including Y-shaped bacteria, indicating a possible general weakening of the cell wall. The FtsL protein is estimated to be present at about 20 to 40 copies per cell. The periplasmic domain of the protein displays a sequence with features characteristic of leucine zippers, which are involved in protein dimerization.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

FtsL, an Essential Cytoplasmic Membrane Protein Involved in Cell Division in Escherichia coli

1992

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“…The main component is FtsZ, which is the most abundant cell division protein, with 4,000 to 15,000 molecules per cell (Table 1) (54,76,78). So, the bulk of the ring lies in the cytoplasmic side, while the membrane and periplasmic parts constitute a smaller fraction, at least in mass terms, because some of its components, PBP3, FtsQ, and FtsL, are found in very small amounts in the cell (5,93) or are very small proteins, such as FtsL and FtsB (Table 1) (15,35).…”

Section: The Division Ringmentioning

confidence: 99%

Septum Enlightenment: Assembly of Bacterial Division Proteins

et al. 2006

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“…Proteins with this topology have been termed bitopic membrane proteins. All three proteins are made in very small amounts, with estimates ranging from 25 to 150 molecules per cell (1,7,11,18).…”

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confidence: 99%