Alkaline phosphatase and maltase activity in the embryonic chick intestine in culture

Black, Betty L.; Moog, Florence

doi:10.1016/0012-1606(78)90287-7

Cited by 70 publications

(35 citation statements)

References 31 publications

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“…cortisone and thyroxine. Indeed the observations of Black and Moog [1] indicating that insulin not only has no effect on maltase and alkaline phosphatase activities of chick embryo small intestine in organ culture but also inhibits the increases of activities induced by cortisone and thyroxine, suggested that this phenome non could be generalized to mammalian small intestine. The present investigation us ing suckling mice confirms that insulin accel erates the development of the brush border membrane hydrolytic activities [20] and shows that there is no antagonism between insulin and cortisone or thyroxine on maltase and alkaline phosphatase activities.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Insulin and Thyroxine on the Differentiation and Proliferation of Epithelial Cells of Suckling Mouse Small Intestine

Malo¹,

Ménard²

1983

Neonatology

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The interactions between insulin, cortisone and thyroxine on the functional maturation of the small intestine were investigated. 8-day-old suckling mice received one injection/day during 3 days of a single hormone or of the following hormonal combinations: insulin plus cortisone or insulin plus thyroxine. The differentiation of intestinal epithelial cells was evaluated by measuring brush border membrane hydrolytic activities and proliferation by establishing the mitotic index and ³H-thymidine incorporation into DNA. The effects of cortisone and insulin on enterocyte’s differentiation are additive. Thyroxine accelerates the differentiation and has a limited action on cell proliferation. Insulin exerts a sustained effect on these two aspects of the intestinal maturation and, furthermore, acts in synergism with thyroxine. It appears that insulin, cortisone and thyroxine have their own specific effect on differentiation and proliferation of the epithelial cells and that these three hormones act in cooperation to induce the maturation of the suckling mouse small intestine during postnatal development.

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Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Insulin and Thyroxine on the Differentiation and Proliferation of Epithelial Cells of Suckling Mouse Small Intestine

Malo¹,

Ménard²

1983

Neonatology

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“…The cells of the connective tissue through their interaction with the epithelium via connective tissue matrix are in a position to mediate influences on epithelial cells. Thus, corticosteroids, for example, may affect epithelial cell differentiation, both directly (28) or indirectly (29). Cytokines, also, may have different effects on the epithelium in contact with fibroblasts than in enterocytes alone (30).…”

Section: Discussionmentioning

confidence: 99%

Human fetal enterocytes in vitro: modulation of the phenotype by extracellular matrix.

Sanderson

Ezzell

Kédinger

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

117

101

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The differentiation of small intestinal epithelial cells may require stimulation by microenvironmental factors in vivo. In this study, the effects of mesenchymal and luminal elements in nonmalignant epithelial cells isolated from the human fetus were studied in vitro. Enterocytes from the human fetus were cultured and microenvironmental factors were added in stages, each stage more closely approximating the microenvironment in vivo. Four stages were exam-

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“…Furthermore, insulin exerted a sustained effect on crypt cell proliferation [17], It is, however, difficult to conclude whether or not these modifications resulted from a direct action of insulin on the intestinal mucosa or represented a response to a systemic reaction such as for example the transient hypoglycemia caused by insulin [ 19], Because organ culture removes the stud ied tissue from the interacting complexities of the intact organism, it permits a more sys tematic approach to the study of the intrinsic and extrinsic regulators of intestinal epithe lial maturation than is possible in vivo [11,30]. This methodology made it possible to investigate the ontogenetic development of the intestine of the fetal rat, mouse and chick [3,5,6], Few investigations have been de voted to the analysis of intestinal develop-mcnt in vitro using suckling intestine [ 12,28,29]. Since we recently reported the successful maintenance of suckling mouse small intes tine in a serum-free medium [ 16], the present investigation was undertaken to determine the possible direct effect of insulin on the development of the hydrolytic functions of the brush border and on the proliferation of the crypt cells of suckling mouse small intes tine maintained in organ culture.…”

Section: Introductionmentioning

confidence: 99%

Insulin Influences the Maturation and Proliferation of Suckling Mouse Intestinal Mucosa in Serum-Free Organ Culture

Arsenault

Ménard²

1984

Neonatology

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Explants of suckling mouse jejunum have been maintained in serum-free organ culture with or without insulin added to the medium in order to determine the possible direct effect of this hormone on the hydrolytic functions of the brush border and on the proliferation of the crypt cells. The addition of insulin induced the precocious appearance of sucrase activity and increased trehalase, glucoamylase and lactase activities. Alkaline phosphatase activity remained unaffected in the tissue as well as in the medium. An increased DNA content and ³H-thymidine incorporation into DNA were already recorded after 24 h of culture. The mitotic index was significantly increased after 24 h and remained elevated when the culture was extended to 48 h. These results show that insulin directly influences the enzymatic maturation and the proliferation of intestinal epithelial cells of suckling mouse.

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Alkaline phosphatase and maltase activity in the embryonic chick intestine in culture

Cited by 70 publications

References 31 publications

Synergistic Effects of Insulin and Thyroxine on the Differentiation and Proliferation of Epithelial Cells of Suckling Mouse Small Intestine

Synergistic Effects of Insulin and Thyroxine on the Differentiation and Proliferation of Epithelial Cells of Suckling Mouse Small Intestine

Human fetal enterocytes in vitro: modulation of the phenotype by extracellular matrix.

Insulin Influences the Maturation and Proliferation of Suckling Mouse Intestinal Mucosa in Serum-Free Organ Culture

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