Alkaline phosphatase and bone calcium parameters

Fauran-Clavel, M.J.; Oustrin, J

doi:10.1016/8756-3282(86)90680-0

Cited by 20 publications

(8 citation statements)

References 23 publications

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“…During mineralization and osteogenic differentiation a higher amount of proteins is expressed [40]. Increased conversion of ALP corresponds to increased osteogenic activity [41]. The present study determined the conversion of ALP normalized to the total expressed protein according to the method described by Liu et al and Zhao et al [18,31].…”

Section: Discussionmentioning

confidence: 86%

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Sabandal¹,

Schäfer²,

Aed³

et al. 2020

Head Face Med

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Background: Frequently statins were administered to reduce the LDL-concentration in circulating blood. Especially simvastatin (SV) is an often prescribed statin. Pleiotropic effects of these drugs were reported. Thus, the aim of this study was to evaluate effects of SV on osteoblastic mineralization. Methods: After informed consent primary osteoblasts were collected from tissue surplus after treatment of 14 individuals in the Department of Cranio-Maxillofacial Surgery, University Hospital Münster. The cells were passaged according to established protocols. Viability, mineralization capability and osteoblastic marker (alkaline phosphatase) were determined at day 9, 13 and 16 after adding various SV concentrations (0.05 μM, 0.1 μM, 0.5 μM, 1.0 μM). Statistical analysis was performed using the Kruskal-Wallis-test. Results: The cell cultures showed a time and dose-dependent significantly decreased viability (p < 0.01) and a significantly increased mineralization (p < 0.01) in a late mineralization stage after adding SV. The typical alteration of the alkaline phosphatase (ALP) levels during osteogenic differentiation was not recognizable. Conclusions: The pleiotropic effects found for different SV concentrations were possibly originated from other mineralization pathways beside the ALP induced one. Additionally, possible alterations of protein expression levels during mineralization and investigation of possible deviating application of SV in other treatment fields can be considered after gaining a deeper insight in the affected mechanisms.

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Section: Discussionmentioning

confidence: 86%

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Sabandal¹,

Schäfer²,

Aed³

et al. 2020

Head Face Med

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“…In good agreement with this result, ALPase activity also appeared defective in UCWJ cells. Alkaline phosphatase activity has been reported to correlate with the degree of calcification [29]. The cells derived from UCWJ differentiated into chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s jelly explants of human umbilical cord

et al. 2009

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We isolated mesenchymal stem cells (MSC) from arteries (UCA), veins (UCV), and Wharton's jelly (UCWJ) of human umbilical cords (UC) and determined their relative capacities for sustained proliferation and multilineage differentiation. Individual UC components were dissected, diced into 1-2 mm(3) fragments, and aligned in explant cultures from which migrating cells were isolated using trypsinization. Preparations from 13 UCs produced 13 UCWJ, 11 UCV, and 10 UCA cultures of fibroblast-like, spindle-shaped cells negative for CD31, CD34, CD45, CD271, and HLA-class II, but positive for CD13, CD29, CD44, CD73, CD90, CD105, and HLA-class I. UCV cells exhibited a significantly higher frequency of colony-forming units fibroblasts than did UCWJ and UCA cells. Individual MSCs could be selectively differentiated into osteoblasts, chondrocytes, and adipocytes. When compared for osteogenic potential, UCWJ cells were the least effective precursors, whereas UCA-derived cells developed alkaline phosphatase activity with or without an osteogenic stimulus. UC components, especially blood vessels, could provide a promising source of MSCs with important clinical applications.

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“…Romagnoli et al (31) reported that the expression of ALP in bones is useful in clinical practice. ALP can be used as a marker to assess osteoblast activity and decreased serum ALP activity reduces osteogenesis (32,33). In the present study, HG stimulation decreased the activity and expression of ALP, suggesting that HG reduced osteogenesis.…”

Section: Discussionmentioning

confidence: 99%

High glucose inhibits osteogenic differentiation and proliferation of MC3T3‑E1 cells by regulating P2X7

Yang

Zhang

2019

Mol Med Report

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Diabetes mellitus adversely affects human bones and increases the risk of developing osteoporosis. In the present study, treatment with 30 mmol/l glucose was used to establish a high glucose (HG) cell model in vitro. Plasmids were used to overexpress the P2X purinoceptor 7 (P2X7) gene. Brilliant blue G and (4-benzoyl-benzoyl)-ATP were used as a P2X7 antagonist and agonist, respectively. Proliferation of osteogenic MC3T3-E1 cells and alkaline phosphatase (ALP) activity were determined using MTT and colorimetric assays, respectively. Alizarin Red S was used to assess calcification of MC3T3-E1 cells. Western blotting and reverse transcription-quantitative PCR were performed to determine protein and mRNA expression levels. The results demonstrated that HG inhibited MC3T3-E1 cell proliferation and P2X7 expression, reduced calcification, and downregulated the expression levels of ALP and osteocalcin (Ocn) in MC3T3-E1 cells. Overexpression of P2X7 in HG conditions increased calcification and proliferation, and upregulated the levels of ALP and Ocn in MC3T3-E1 cells. Inhibition of P2X7 downregulated the expressions of ALP and Ocn in MC3T3-E1 cells under HG conditions. Therefore, the present results indicated that HG caused damage to osteogenic MC3T3-E1 cells. Thus, P2X7 may be a regulatory factor that may be used to counteract the effects of HG on osteogenesis.

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Alkaline phosphatase and bone calcium parameters

Cited by 20 publications

References 23 publications

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts

Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s jelly explants of human umbilical cord

High glucose inhibits osteogenic differentiation and proliferation of MC3T3‑E1 cells by regulating P2X7

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