Alkaline phophatase from the hepatopancreas of shrimp (Pandalus borealis): A dimeric enzyme with catalytically active subunits

Crystal Structure of Alkaline Phosphatase from the Antarctic Bacterium TAB5

“…3 The AP from Antarctic strain TAB5 (TAP) is inactivated completely at 50°C (15 min incubation), while the traditional calf intestinal AP (CIP) retains 90% of its activity when treated in the same way. 4 In addition to TAP, coldadapted APs from Atlantic cod (Gadus morhua), 5 a marine Vibrio sp, 6 the bacterial strain HK47, 7 a Shewanella sp, 8 and shrimp (Pandalus borealis, SAP), 9,10 have been isolated. Both SAP and TAP are commercially available and are used for in vitro reactions.…”

A Moderately Thermostable Alkaline Phosphatase from Geobacillus thermodenitrificans T2: Cloning, Expression and Biochemical Characterization

“…Moreover, the enzyme also exhibited at broad high activity (more than 62% maximal activity) at temperatures ranging from 45°to 65°C, which is consistent with the temperature for the survival of Geobacillus species. The purified recombinant Gtd AP was found to retain a relatively high activity after incubation of 60°C for 1 h. This result showed that this enzyme was more stable than AP from shrimp [31], calf intestine [32], Bacillus stearothermophilus [33], and most psychrophiles [8,[34][35][36][37][38]. These thermal sensitive APs significantly lost activity after the incubation at 60°C.…”

Purification and biochemical properties of highly efficient alkaline phosphatase from Fenneropenaeus merguiensis brain

“…The catalytic mechanism involves the formation of a serine phosphate at the active site, which reacts with water at alkaline pH to release P i from the enzyme [4,5]. Alkaline phosphatases are widely distributed in nature and knowledge of the alkaline phosphatases has increased remarkably during the last few years [6]. ALPs in marine organisms play important roles in cell phosphate metabolism, which is related to the absorption of phosphate and calcium from seawater and the biomineralization process in marine organisms [7,8].…”