Alkaline hydrolysis of olive fruits wastes for the production of single cell protein by Candida lipolytica

Rages, Ashraf Anwar; Haider, Mustafa M.; Aydin, Miraç

doi:10.1016/j.bcab.2021.101999

Cited by 26 publications

(16 citation statements)

References 15 publications

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“…In these conditions the yeast produced 40-50 % of the protein in its dry cell weight cultivated on medium based on wastes from biodiesel production (vegetable oils, degumming, and glycerol fractions) [15]. These environmental conditions appropriate for Y. lipolytica cultivation in olive fruits waste-based medium is consistent with another study by Rages et al, [46], which observed the highest Environmental and Climate Technologies ____________________________________________________________________________ 2022 / 26 protein concentration of about 71 %, which has been reached at 30 °C and pH 5. With an increase in pH from 5.5 to7.5, a decrease in the amount of protein in the biomass was observed.…”

Section: Temperature and Phsupporting

confidence: 88%

“…With an increase in pH from 5.5 to7.5, a decrease in the amount of protein in the biomass was observed. In contrast, media with an initial pH of 6.5 resulted in a higher biomass yield, with 55 % content of protein [46]. Kot et al [74] noted that the pH value in the range of 4.0-7.0 did not significantly affect the amount of protein in the R. glutinis biomass, which varied from 38.5 % to 41.3 % after 72 h of cultivation.…”

Section: Temperature and Phmentioning

confidence: 99%

“…According to the literature, the main factors that affect yeast biomass growth and SCP content are pH, temperature, time of cultivation and requirements for carbon, nitrogen, and trace elements. [32], [43]- [46].…”

Section: Influencing Factors On Yeast Scp Productionmentioning

confidence: 99%

“…Yarrowia lipolytica, another specie of yeast, commonly used for single-cell oil production, however, can also be used for single-cell protein production [24]. Studies report high protein content in the cells of the preceding species, about 46-71 % [24], [46]. Other yeast species such as Rhodotorula glutinis, Pichia stipitis, Blastobotrys adeninivorans and Wickerhamomyces anomalus show satisfactory results, 30-46 % of protein content [28], [34], [57].…”

Section: Selection Of Yeast Speciesmentioning

confidence: 99%

“…Another important factor in successful yeast cultivation is the choice of temperature and pH of medium. According to the literature, the most common temperature used for protein biosynthesis by yeast species is 25-30 °C [22], [24], [46], [64], [73]; however, for some yeasts, such C. utilis and K. marxianus a temperature of 35 and 40 °C can be used [58]. Low temperature can inhibit nutrients from crossing the cell membrane, while a high temperature may inactivate enzymes of the metabolic pathway [22].…”

Section: Temperature and Phmentioning

confidence: 99%

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Optimization of Yeast Cultivation Factors for Improved SCP Production

Raita

Kusnere

Spalviņš

et al. 2022

Environmental and Climate Technologies

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Yeast Single-Cell Proteins (SCP) production using various agro-industrial byproducts and wastes have significant potential as an alternative to the soy meal, and fish meal protein used for livestock and aquaculture feeds. The use of organic wastes as a substrate in the fermentation processes can be accepted as one of the solutions to reduce the total price of the culture and an environmentally friendlier method of removing these residues. This review article focuses on the yeast biomass yield and protein content increase strategies, which is impossible without understanding metabolic pathways and switching mechanisms. The present work discusses optimization strategies for protein-enriched yeast biomass production, such as fermentation medium composition, including a selection of carbon and nitrogen sources and their ratio, supplemented trace elements, and cultivation conditions such as pH, temperature, time of cultivation, and inoculum size. This review summarizes the theoretical knowledge and experimental results of other researchers that provide an overview of the achievements of the last decades in the production of SCP.

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Section: Temperature and Phsupporting

confidence: 88%

Section: Temperature and Phmentioning

confidence: 99%

Section: Influencing Factors On Yeast Scp Productionmentioning

confidence: 99%

Section: Selection Of Yeast Speciesmentioning

confidence: 99%

Section: Temperature and Phmentioning

confidence: 99%

See 3 more Smart Citations

Optimization of Yeast Cultivation Factors for Improved SCP Production

Raita

Kusnere

Spalviņš

et al. 2022

Environmental and Climate Technologies

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Opportunities and challenges in single‐cell protein production using lignocellulosic material

Kumar,

Raj,

Næss

et al. 2023

Biofuels Bioprod Bioref

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The increase in world population has led to an increase in global food demand, which is estimated to grow by 35–56% from 2010 to 2050. As a result, the risk of hunger is also expected to rise. Protein consumption between 2000 and 2018 rose by 40% worldwide and is expected to increase at 9.3% cumulative annual growth rate in the near future. Hence, there is a huge demand and potential market available for human and animal protein. Therefore, newer and more protein sources will be required to meet the longer food value chains. Hence, a microbial protein produced using waste biomass could offer socio‐economic benefits and consumer acceptance owing to its vegetarian nature. Moreover, waste biomass‐based proteins are environmentally benign and very good ingredients in food and feed. Dry microbial protein, described as single‐cell protein, grown on agricultural residue, has been considered as a potent source of protein for both humans and animals. Thus, single‐cell protein will open up new opportunities to meet the growing protein demands for humans and animals. Microbially produced beer, cheese and yogurt are all examples of microbial protein sources for humans and animals. The feedstock, like agriculture residue, is relatively cheap and could be a potential source for large‐scale protein production in the future.

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Reusing wastewater from Coffea arabica processing to produce single‐cell protein using Candida sorboxylosa: Optimizing of culture conditions

Pillaca‐Pullo,

Lopes,

Estela‐Escalante

2023

Biotechnology Progress

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Coffee is a crop of significant socioeconomic importance, and the reuse of agri‐food by‐products and biowaste has great potential across several industries. Coffee wastewater (CWW) is a valuable resource containing essential nutrients that can be utilized by Candida sorboxylosa for single‐cell protein (SCP) production. This utilization contributes to mitigating the negative impacts of agro‐industrial waste. The optimization of culture conditions using the design of experiments (DoE) technique is crucial in understanding the environmental factors influencing metabolite production. In our study, the DoE technique was employed to analyze culture conditions, including room temperature, pH 8.4, agitation at 200 rpm, a headspace of 60% (v/v), and an inoculum of 0.75 DO600nm over 28‐h period. This approach resulted in a remarkable SCP yield of 64.4% and dry cell weight (DCW) of 2.26 g/L. It is noteworthy that there is no literature reporting SCP production under alkaline pH conditions in yeast. Interestingly, our work demonstrated that an alkaline pH of 8.4 significantly influenced SCP production by C. sorboxylosa. The DoE technique proved to be an efficient statistical tool for optimizing culture conditions, offering several advantages, such as: (i) conducting cultures at room temperature to minimize unnecessary energy consumption; (ii) reducing the incubation time from 46 to 28 h, thereby enhancing overall productivity; (iii) achieving 1.7‐fold increase in SCP yield compared to previous basal production levels.

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Alkaline hydrolysis of olive fruits wastes for the production of single cell protein by Candida lipolytica

Cited by 26 publications

References 15 publications

Optimization of Yeast Cultivation Factors for Improved SCP Production

Optimization of Yeast Cultivation Factors for Improved SCP Production

Opportunities and challenges in single‐cell protein production using lignocellulosic material

Reusing wastewater from Coffea arabica processing to produce single‐cell protein using Candida sorboxylosa: Optimizing of culture conditions

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