Alkaline deacetylation as a strategy to improve sugars recovery and ethanol production from rice straw hemicellulose and cellulose

Castro, Rafael Cunha de Assis; Fonseca, Bruno Guedes; Santos, Hilton Túlio Lima dos; Ferreira, Isabela Silveira; Mussatto, Solange I.; Roberto, Inês Conceição

doi:10.1016/j.indcrop.2016.08.053

Cited by 79 publications

(30 citation statements)

References 32 publications

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“…29,38 On the other hand, glucan, xylan, arabinan and ash contents of bagasse do not appear significantly altered as percentage of native dry matter (Table 2). Noteworthy, contrasting with our results, previous studies reported reduction of ash contents after deacetylation, 39,40 suggesting dependence on the type of the minerals present in the biomass. It is also important to note that the preservation of components differs in sugar cane straw.…”

Section: Apêndice Acontrasting

confidence: 99%

Produção de etanol celulósico por >i<Spathaspora passalidarum>/i< a partir do bagaço de cana-de-açúcar: estratégia para melhorar fermentabilidade e estudo do perfil metabólico durante fermentação de hidrolisado hemicelulósico

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Section: Apêndice Acontrasting

confidence: 99%

Produção de etanol celulósico por >i<Spathaspora passalidarum>/i< a partir do bagaço de cana-de-açúcar: estratégia para melhorar fermentabilidade e estudo do perfil metabólico durante fermentação de hidrolisado hemicelulósico

Lima¹

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“…Mechanical refining further improves the digestibility of deacetylated biomass by increasing the cellulose accessibility with cutting and fibrillation (Jones et al, , 2014Kim S. M. et al, 2016;Park et al, 2016). The DMR pre-treated biomass has shown good digestibility and superb fermentability as reported previously (Zhang et al, 2014;Chen et al, 2016;de Assis Castro et al, 2017).…”

Section: Introductionmentioning

confidence: 55%

Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover

et al. 2019

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The National Renewable Energy Laboratory (NREL) developed low severity Deacetylation and Mechanical Refining (DMR) process under atmospheric pressure, generates highly fermentable, low toxicity sugar syrups and highly reactive lignin streams. The dilute alkali deacetylation step saponifies acetyl groups from the hemicellulosic fraction of biomass into a black liquor waste stream that also contains solubilized acetic, ferulic, p-coumaric acids, lignin-carbohydrate complexes (LCCs), oligosaccharides, and solubilized lignin. Valorization of the soluble components in the deacetylation black liquor waste stream requires characterization of the black liquor. Our analyses show that glucan, xylan, and lignin were three main components in black liquor and the oligosaccharides in the black liquor were mainly dimers and trimers with arabinofuranose branch groups mainly on the xylooligomers. GPC chromatograms showed that the black liquor also contained oligomeric lignin moieties with molecular weights ranging between 1,800 and ∼10,000 Da. Enzymatic saccharification of the black liquor was conducted based on the compositional and structural information. Incorporation of commercial cellulase and hemicellulase enzyme preparations with various accessary enzymes showed up to 57% hydrolysis of the xylooligosaccharides in the black liquor to monomeric sugars, which could be used to improve biorefinery carbon utilization in biological fermentations to improve end product fuel or bioproduct volumes per metric ton of biomass processed. This is so far the first study about characterizing DMR black liquor and exploring breaking down the oligosaccharides in black liquor for potential use.

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“…The biomass was milled (1 cm length per 1 mm thickness) in a hammer-mill and stored at room temperature. The pre-treatment of rice straw consisted of an alkaline step (deacetylation) using 80 mg NaOH/g of biomass, at 70 • C and 45 min, followed by a dilute acid step using 100 mg H 2 SO 4 /g deacetylated rice straw, at 121 • C for 85 min, as described by Castro et al [24]. Both treatments were performed in a 50-L batch reactor with a liquid-to-solid ratio of 10:1 (mL/g).…”

Section: Raw Materials Preparationmentioning

confidence: 99%

Ethanol Production from High Solid Loading of Rice Straw by Simultaneous Saccharification and Fermentation in a Non-Conventional Reactor

et al. 2020

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Simultaneous saccharification and fermentation (SSF) at high solid loading is a potential approach to improve the economic feasibility of cellulosic ethanol. In this study, SSF using high loading of rice straw was assessed using a vertical ball mill reactor. First, the conditions of temperature and number of glass spheres were optimized at 8% (w/v) initial solids (41.5 °C, 18 spheres). Then, assays were carried out at higher solid loadings (16% and 24% w/v). At 8% or 16% solids, the fermentation efficiency was similar (ηF~75%), but the ethanol volumetric productivity (QP) reduced from 1.50 to 1.14 g/L.h. By increasing the solids to 24%, the process was strongly affected (ηF = 40% and QP = 0.7 g/L.h). To overcome this drawback, three different feeding profiles of 24% pre-treated rice straw were investigated. Gradual feeding of the substrate (initial load of 16% with additions of 4% at 10 and 24 h) and an inoculum level of 3 g/L resulted in a high ethanol titer (52.3 g/L) with QP of 1.1 g/L.h and ηF of 67%. These findings demonstrated that using a suitable fed-batch feeding strategy helps to overcome the limitations of SSF in batch mode caused by the use of high solid content.

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Alkaline deacetylation as a strategy to improve sugars recovery and ethanol production from rice straw hemicellulose and cellulose

Cited by 79 publications

References 32 publications

Produção de etanol celulósico por >i<Spathaspora passalidarum>/i< a partir do bagaço de cana-de-açúcar: estratégia para melhorar fermentabilidade e estudo do perfil metabólico durante fermentação de hidrolisado hemicelulósico

Produção de etanol celulósico por >i<Spathaspora passalidarum>/i< a partir do bagaço de cana-de-açúcar: estratégia para melhorar fermentabilidade e estudo do perfil metabólico durante fermentação de hidrolisado hemicelulósico

Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover

Ethanol Production from High Solid Loading of Rice Straw by Simultaneous Saccharification and Fermentation in a Non-Conventional Reactor

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