Alkaline-active xylanase produced by an alkaliphilicBacillus sp isolated from kraft pulp

Yang, Vina W.; Zhuang, Ziyu; Elegir, Graziano; Jeffries, Thomas W.

doi:10.1007/bf01569971

Cited by 77 publications

(46 citation statements)

References 36 publications

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“…The brewing industry uses xylanases to reduce viscosity and improve filtration rate (Shi et al 2010). The pulp and paper industry has also made use of xylanases for selective degradation in cellulosic pulps to remove residual xylans in brownstock holding tanks and also in deinking (Singh et al 2007;Yang et al 1995). Xylanases can also improve bread quality by increasing volume; enhance clarification of juices; facilitate extraction of coffee plant oils and starch; and degumming flax, hemp, or jute fibers (Beg et al 2001).…”

Section: Hemicellulosementioning

confidence: 97%

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Bacterial biodegradation and bioconversion of industrial lignocellulosic streams

Mathews

Pawlak

Grunden

2015

Appl Microbiol Biotechnol

104

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Lignocellulose is a term for plant materials that are composed of matrices of cellulose, hemicellulose, and lignin. Lignocellulose is a renewable feedstock for many industries. Lignocellulosic materials are used for the production of paper, fuels, and chemicals. Typically, industry focuses on transforming the polysaccharides present in lignocellulose into products resulting in the incomplete use of this resource. The materials that are not completely used make up the underutilized streams of materials that contain cellulose, hemicellulose, and lignin. These underutilized streams have potential for conversion into valuable products. Treatment of these lignocellulosic streams with bacteria, which specifically degrade lignocellulose through the action of enzymes, offers a low-energy and low-cost method for biodegradation and bioconversion. This review describes lignocellulosic streams and summarizes different aspects of biological treatments including the bacteria isolated from lignocellulose-containing environments and enzymes which may be used for bioconversion. The chemicals produced during bioconversion can be used for a variety of products including adhesives, plastics, resins, food additives, and petrochemical replacements.

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Section: Hemicellulosementioning

confidence: 97%

“…Few xylan-degrading alkaliphiles have been identified; however, Bacillus sp. (V1-4), isolated from hardwood kraft pulp, produced xylanase at pH 9 under mesophilic temperatures and aerobic conditions (Yang et al 1995). This enzyme is promising for use in biological treatment of lignocellulose streams.…”

Section: Hemicellulosementioning

confidence: 99%

Bacterial biodegradation and bioconversion of industrial lignocellulosic streams

Mathews

Pawlak

Grunden

2015

Appl Microbiol Biotechnol

104

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“…Positive xylanolytic isolates were detected based on the clear zone of hydrolysis on the MEA xylan agar plate. Further confirmation of potential xylanase producers was carried out using the defined xylan agar (DX) media (Birch wood xylan 1.0%, KH 2 PO 4 0.1%, (NH 4 ) 2 SO 4 0.2%, NaCl 0.1%, agar-agar 2.0%) [14]. The isolates obtained were further screened for xylanase activities by cultivating in DX broth for 12 days under shaking condition.…”

Section: Isolation and Identification Of Xylanolytic Microorganismsmentioning

confidence: 99%

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Pandya¹,

Gupte²

2012

Bioprocess Biosyst Eng

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The production of extracellular xylanase by a locally isolated strain of Aspergillus tubingensis JP-1 was studied under solid-state fermentation. Among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production. The influence of various parameters such as initial pH, moisture, moistening agents, nitrogen sources, additives, surfactants and pretreatment of substrates were investigated. The production of the xylanase reached a peak in 8 days using untreated wheat straw with modified MS medium, pH 6.0 at 1:5 moisture level at 30 °C. Under optimized conditions yield as high as 6,887 ± 16 U/g of untreated wheat straw was achieved. Crude xylanase was used for enzymatic saccharification of agro-residues like wheat straw, rice bran, wheat bran, sugarcane bagasse and industrial paper pulp. Dilute alkali (1 N NaOH) and acid (1 N H(2)SO(4)) pretreatment were found to be beneficial for the efficient enzymatic hydrolysis of wheat straw. Dilute alkali and acid-pretreated wheat straw yielded 688 and 543 mg/g reducing sugar, respectively. Yield of 726 mg/g reducing sugar was obtained from paper pulp after 48 h of incubation.

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“…AR-135, Bacillus sp. strain 41M-1 have been shown to produce xylanases having good activity at a pH greater than 8 and an optimum temperature for activity and stability at or below 50-55°C (Gessesse and Mamo, 1998;Nakamura et al, 1993;Yang et al, 1995). A highly active, thermostable, cellulase-free and alkali-tolerant xylanase from S. racemosum Cohn.…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of a thermostable-cellulase free xylanase from Syncephalastrum racemosum Cohn.

Sapre

Jha

Patil

2005

J. Gen. Appl. Microbiol.

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Syncephalastrum racemosum Cohn. produces an extracellular xylanase that was shown to potentially bleach pulp at pH 10 and 50°C. The enzyme was found to be a dimer with an apparent molecular weight of 29 kDa as determined by SDS-PAGE. The optimum activity was found at two pH values 8.5 and 10.5; however the activity sharply decreased below pH 6 and above pH 10.5. The enzyme was stable for 72 h at pH 10.5 and at 50°C. Kinetic experiments at 50°C gave V max and K m of 1,400 U/ml min ؊1 mg ؊1 protein and 0.05 mg/ml respectively. The enzyme had no apparent requirement for cofactors, and its activity was strongly inhibited by group II b metal ions like Zn 2؉, Hg 2؉, etc. Xylan completely protected the enzyme from being inactivated by N-bromosuccinimide.

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Alkaline-active xylanase produced by an alkaliphilicBacillus sp isolated from kraft pulp

Cited by 77 publications

References 36 publications

Bacterial biodegradation and bioconversion of industrial lignocellulosic streams

Bacterial biodegradation and bioconversion of industrial lignocellulosic streams

Production of xylanase under solid-state fermentation by Aspergillus tubingensis JP-1 and its application

Purification and characterization of a thermostable-cellulase free xylanase from Syncephalastrum racemosum Cohn.

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