Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro

Iwamoto, Atsushi; Inoue, Yuichi; Tachibana, Hirofumi; Kawahara, Hiroharu

doi:10.1007/s10616-019-00306-5

Cited by 9 publications

(9 citation statements)

References 27 publications

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“…Pectin containing more than 80% galacturonic acid residues was found both to decrease macrophage activity and inhibit delayed-type hypersensitivity reactions [50]. Moreover, alkali-soluble pectin suppressed IgE production in a human myeloma cell line in vitro [112]. Other results also indicate that administration of Asian pear pectin-sol (a pH and enzymatically modified pectin in colloidal dispersion) in sensitized mice suppressed allergic asthmatic reactions [122].…”

Section: Effect Of Pectin On the Immune Response And Allergic Sensitizationmentioning

confidence: 96%

The Dietary Fiber Pectin: Health Benefits and Potential for the Treatment of Allergies by Modulation of Gut Microbiota

Blanco-Pérez

Steigerwald

Schülke

et al. 2021

Curr Allergy Asthma Rep

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Purpose of Review The incidence of allergies is increasing and has been associated with several environmental factors including westernized diets. Changes in environment and nutrition can result in dysbiosis of the skin, gut, and lung microbiota altering the production of microbial metabolites, which may in turn generate epigenetic modifications. The present review addresses studies on pectin-mediated effects on allergies, including the immune modulating mechanisms by bacterial metabolites. Recent Findings Recently, microbiota have gained attention as target for allergy intervention, especially with prebiotics, that are able to stimulate the growth and activity of certain microorganisms. Dietary fibers, which cannot be digested in the gastrointestinal tract, can alter the gut microbiota and lead to increased local and systemic concentrations of gut microbiota-derived short chain fatty acids (SCFAs). These can promote the generation of peripheral regulatory T cells (Treg) by epigenetic modulation and suppress the inflammatory function of dendritic cells (DCs) by transcriptional modulation. The dietary fiber pectin (a plant-derived polysaccharide commonly used as gelling agent and dietary supplement) can alter the ratio of Firmicutes to Bacteroidetes in gut and lung microbiota, increasing the concentrations of SCFAs in feces and sera, and reducing the development of airway inflammation by suppressing DC function. Summary Pectin has shown immunomodulatory effects on allergies, although the underlying mechanisms still need to be elucidated. It has been suggested that the different types of pectin may exert direct and/or indirect immunomodulatory effects through different mechanisms. However, little is known about the relation of certain pectin structures to allergies.

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Section: Effect Of Pectin On the Immune Response And Allergic Sensitizationmentioning

confidence: 96%

The Dietary Fiber Pectin: Health Benefits and Potential for the Treatment of Allergies by Modulation of Gut Microbiota

Blanco-Pérez

Steigerwald

Schülke

et al. 2021

Curr Allergy Asthma Rep

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“…However, whereas OP altered cell viability, WP, HXP, and HP did not. Moreover, pectin increased the proportion of IL-2 receptor (IL-2R)-positive cells in mesenteric lymph node (MLN) lymphocytes, indicating that its immunoglobulin modifying effect was mediated by activated Th1 cells [ 58 ].…”

Section: Anticancer Properties Of Pectinmentioning

confidence: 99%

Pectin: A Bioactive Food Polysaccharide with Cancer Preventive Potential

et al. 2022

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Pectin is an acidic heteropolysaccharide found in the cell walls and the primary and middle lamella of land plants. To be authorized as a food additive, industrial pectins must meet strict guidelines set forth by the Food and Agricultural Organization and must contain at least 65% polygalacturonic acid to achieve the E440 level. Fruit pectin derived from oranges or apples is commonly used in the food industry to gel or thicken foods and to stabilize acid-based milk beverages. It is a naturally occurring component and can be ingested by dietary consumption of fruit and vegetables. Preventing long-term chronic diseases like diabetes and heart disease is an important role of dietary carbohydrates. Colon and breast cancer are among the diseases for which data suggest that modified pectin (MP), specifically modified citrus pectin (MCP), has beneficial effects on the development and spread of malignancies, in addition to its benefits as a soluble dietary fiber. Cellular and animal studies and human clinical trials have provided corroborating data. Although pectin has many diverse functional qualities, this review focuses on various modifications used to develop MP and its benefits for cancer prevention, bioavailability, clinical trials, and toxicity studies. This review concludes that pectin has anti-cancer characteristics that have been found to inhibit tumor development and proliferation in a wide variety of cancer cells. Nevertheless, further clinical and basic research is required to confirm the chemopreventive or therapeutic role of specific dietary carbohydrate molecules.

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“…■ ABBREVIATIONS IgE, immunoglobulin E; FcεRI, high-affinity IgE receptor; DMEM, Dulbecco's modified Eagles' medium; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; CCK-8, Cell Count Kit-8; HRP, horseradish peroxidase; TLC, thin layer chromatography; FBS, fetal bovine serum; PBS, phosphate buffered saline; OVA, ovalbumin; ELISA, enzyme-linked immunosorbent assay; PIP3, phosphatidylinositol (3,4,5)-trisphosphate…”

Section: Author Contributionsmentioning

confidence: 99%

“…Hence, plant-based therapeutics have gained popularity as relatively safe options. For instance, food components such as methylated catechin, strictinin, pectin, and metabolic enzymes are known to exhibit anti-allergic properties. − Allergic diseases such as allergic asthma, rhinitis, and atopic dermatitis are categorized as type I hypersensitivity reactions that are provoked by the degranulation of granulocytes, including mast cells and basophils. − Immunoglobulin (IgE) binds to high-affinity IgE receptor (FcεRI) expressed on granulocytes, and the cross-linking between an allergen and IgE to FcεRI results in the release of inflammatory mediators such as histamine, leukotriene, cytokines, and chemokines through degranulation. The binding of a specific allergen to a receptor-bound IgE may increase the concentration of intracellular Ca 2+ , leading to the granulocyte-mediated release of preformed mediators and arachidonic acid metabolites.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Green Asparagus Extract, Especially Phospholipids, on Allergic Responses in Vitro and in Vivo

Iwamoto¹,

Hamajima²,

Tsuge³

et al. 2020

J. Agric. Food Chem.

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Asparagus (Asparagus officinalis L.) is one of the widely consumed vegetables. To investigate the mechanism underlying the anti-allergic responses of asparagus, we extracted different fractions from asparagus and measured their inhibitory effects on β-hexosaminidase release in RBL-2H3 cells in vitro and an atopic dermatitis NC/Nga mouse model in vivo. The lipid fractions from asparagus were extracted with 50% ethanol, separated using chloroform by liquid–liquid phase separation, and fractionated by solid-phase extraction. Among them, acetone fraction (rich in glycolipid) and MeOH fraction (rich in phospholipid) markedly inhibited β-hexosaminidase release from RBL-2H3 cells. In NC/Nga mice treated with picryl chloride, atopic dermatitis was alleviated following exposure to the 50% EtOH extract, acetone fraction, and methanol fraction. The inhibitory effects of asparagus fractions in vivo were supported by the significant decrease in serum immunoglobulin E (IgE) levels. The phospholipid fractions showed significantly better inhibitory effects, and phosphatidic acid from this fraction showed the best inhibitory effect on β-hexosaminidase release. In mice challenged with ovalbumin (OVA), oral administration of asparagus extract and its fractions decreased the OVA-specific IgE level and total IgE, indicating that these effects may be partly mediated through the downregulation of antigen-specific IgE production. Taken together, the present study shows for the first time that asparagus extract and its lipid fractions could potentially mitigate allergic reactions by decreasing degranulation in granulocytes. Our study provides useful information to develop nutraceuticals and functional foods fortified with asparagus.

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Alkali-soluble pectin suppresses IgE production in human myeloma cell line in vitro

Cited by 9 publications

References 27 publications

The Dietary Fiber Pectin: Health Benefits and Potential for the Treatment of Allergies by Modulation of Gut Microbiota

The Dietary Fiber Pectin: Health Benefits and Potential for the Treatment of Allergies by Modulation of Gut Microbiota

Pectin: A Bioactive Food Polysaccharide with Cancer Preventive Potential

Inhibitory Effects of Green Asparagus Extract, Especially Phospholipids, on Allergic Responses in Vitro and in Vivo

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