Alkali-Induced Corneal Neovascularization Is Independent of CXCR2-Mediated Neutrophil Infiltration

Abstract

DMEK may provide quick visual rehabilitation in the treatment of corneal endothelial disorders by transplantation of an organ-cultured DM transplanted through a clear corneal tunnel incision. DMEK may be a highly accessible procedure to corneal surgeons, because donor DM sheets can be prepared from preserved corneo-scleral rims.

Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy: review of the first 50 consecutive cases

“…14,15 Twenty-three patients were men and 27 women, ranging from 41 to 88 years of age (Table 1). All patients signed an IRB-approved informed consent.…”

“…17,18 After 1 week of culture, endothelial cell morphology and viability were evaluated and the corneo-scleral buttons were mounted endothelial side up on a custom made holder with a suction cup. Descemet's membrane (DM) was stripped from the posterior stroma, so that a 9.0-10.0 mm diameter flap of posterior DM with its endothelial monolayer was obtained.…”

“…International), and sucked into a custom made injector (Hippocratech, Rotterdam, The Netherlands), to transfer the tissue from the culture medium vial to the anterior chamber. 15,17,18 Using the injector, the donor Descemetroll was inserted into the anterior chamber and the graft was oriented endothelial side down (donor DM facing recipient posterior stroma) by careful, indirect manipulation of the tissue with air and fluid. While maintaining the anterior chamber with fluid and air, the graft was gently spread out over the iris.…”

“…Then, an air bubble was injected underneath the donor DM to position the tissue onto the recipient posterior stroma. 15,17,18 The anterior chamber was completely filled with air for 30-60 min followed by an air-liquid exchange to pressurise the eye. Each surgical procedure was recorded on DVD (Pioneer DVR-RT601H-S, Tokyo, Japan).…”

“…[9][10][11][12] In 1998, we also described a technique for selective transplantation of Descemet membrane through a self-sealing 3.5 mm clear corneal incision, 13,14 tentatively named Descemet membrane endothelial keratoplasty (DMEK). [15][16][17][18] As PK has been the gold standard for keratoplasty for almost a century, 19 an alternative technique should bring major advantages in clinical outcome, reducing complications as well as technical feasibility, to get accepted and to change a longstanding consensus in ophthalmology. The early forms of endothelial keratoplasty, that is, DLEK and DSEK/ DSAEK, may have shown that the visual outcome could compete with PK, whereas the most frequent long-term complications were minimised.…”