Alkali-based pretreatments distinctively extract lignin and pectin for enhancing biomass saccharification by altering cellulose features in sugar-rich Jerusalem artichoke stem

Abstract

Jerusalem artichoke (JA) has been known as a potential nonfood feedstock for biofuels. Based on systems analysis of total 59 accessions, both soluble sugar and ash could positively affect biomass digestibility after dilute sodium hydroxide pretreatment (A). In this study, one representative accession (HEN-3) was used to illustrate its enzymatic digestibility with pretreatments of ultrasonic-assisted dilute sodium hydroxide (B), alkaline peroxide (C), and ultrasonic-assisted alkaline peroxide (D). Pretreatment D exhibited the highest hexose release rate (79.4%) and total sugar yield (10.4 g/L), which were 2.4 and 2.6 times higher, respectively, than those of the control. The analysis of cellulose crystalline index (CrI), cellulose degree of polymerization (DP), thermal behavior and SEM suggested that alkali-based pretreatments could distinctively extract lignin and pectin polymers, leading to significant alterations of cellulose CrI and DP for high biomass saccharification. Additionally, hydrogen peroxide (H2O2) could significant reduce the generation of fermentation inhibitors during alkali-based pretreatments.

Pretreatment of wheat straw leads to structural changes and improved enzymatic hydrolysis

“…Li et al . used four different alkali-based pretreatments to pretreat stems of Jerusalem artichoke and found that alkali pretreatment as the most effective technique in removing non-cellulosic polymers 23 .…”

High-throughput analysis of chemical components and theoretical ethanol yield of dedicated bioenergy sorghum using dual-optimized partial least squares calibration models

“…All sorghum samples were harvested on their dates of physiological maturity after a growth period of between 110 and 150 days. Preparation of samples was performed according to the process described by Li et al [ 3 ] with minor modifications. In this study, stem samples were ground using a crusher mill into particles 1–2 cm in size.…”

“…The soluble sugar was extracted with distilled water and determined by the anthrone/H 2 SO 4 method using a UV–VIS spectrometer (TU-1901, Beijing Purkinje Instruments Co. Ltd., Beijing, China) according to Li et al [ 3 ] The standard curve was plotted using d -glucose as the standard (purchased from Xilong Scientific Co., Ltd., China).…”

“…Interest in biomass as an alternative energy resource has been increasing dramatically in step with increases in fossil energy costs worldwide [ 1 ]. As a promising advanced biofuel, bioethanol has become one of the most practical solutions for carbon emission reduction and food security in recent decades [ 2 , 3 ]. Moreover, production of second-generation bioethanol derived from lignocellulosic agricultural wastes offers farmers and rural communities more economic opportunities than did first-generation bioethanol production [ 4 ].…”

A combined sodium phosphate and sodium sulfide pretreatment for enhanced enzymatic digestibility and delignification of corn stover

“…Lignocellulosic biomass is a plentiful, sustainable, and non-food resource that can be used to produce alternative fuels and valuable chemicals to address the concerns of increasing energy consumption and environmental pollution (Himmel et al, 2007;Li et al, 2016). However, the processing of lignocellulosic biomass is much more challenging than bioconversion of corn starch or sugarcane to liquid fuels, due to the inherent recalcitrance of lignocelluloses to enzymatic or microbial decomposition.…”