Alisol A 24-Acetate and Alisol B 23-Acetate Induced Autophagy Mediates Apoptosis and Nephrotoxicity in Human Renal Proximal Tubular Cells

Wang, Chunfei; Feng, Liang; Ma, Liang; Chen, Haifeng; Tan, Xuecai; Hou, Xuefeng; Song, Jie; Cui, Li; Liú, Dan; Chen, Juan; Yang, Nan; Wang, Jing; Liu, Ying; Zhao, Bingjie; Wang, Gang; Zhou, Yufang; Jia, Xiao‐Bin

doi:10.3389/fphar.2017.00172

Cited by 10 publications

(9 citation statements)

References 46 publications

(44 reference statements)

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“…Juzepzuk (Alismataceae), is a natural medicine widely cultivated in China, Japan, Korea, India, and Europe. Traditionally, AR is known to exhibit diuretic, hyperlipidemic, inflammatory, antitumoral, and damp-heat clearing actions [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ] and has been used for treating dysuria, edema, urinary tract infection, retention of fluid and phlegm, and vertigo for more than 1000 years [ 8 ]. As a folk medicine that has long been used to promote health and longevity, AR is now included in the Pharmacopoeia of China as a representative traditional Chinese herbal diuretic.…”

Section: Introductionmentioning

confidence: 99%

Diuretic Activity of Compatible Triterpene Components of Alismatis rhizoma

Zhang

Lin

et al. 2017

Molecules

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Alismatis rhizoma (AR), the dried rhizoma of Alisma orientale Juzepzuk (Alismataceae), is a traditional Chinese medicine. AR is an important part of many prescriptions and is commonly used as a diuretic agent in Asia. This study aimed to evaluate the diuretic effects of total triterpene extract (TTE) and triterpene component compatibility (TCC, the mixture of alisol B 23-acetate, alisol B, alisol A 24-acetate, alisol A, and alisol C 23-acetate) of AR in saline-loaded rats. The optimal diuretic TCC of AR was optimized using a uniform design. Different doses (5, 20, and 40 mg/kg) of TTE and TCC groups (N1–N8) were orally administered to rats. Urinary excretion rate, pH, and electrolyte excretion were measured in the urine of saline-loaded rats. Results showed that TTE doses increased urine volume and electrolyte excretion compared with the control group. All uniformly designed groups of TCC also increased urine excretion. In addition, optimal diuretic TCC was calculated (alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate 7.2:0.6:2.8:3.0:6.4) and further validated by saline-loaded rats. This study demonstrated that TTE presented a notable diuretic effect by increasing Na+, K+, and Cl− displacements. The most suitable TTC compatible proportion of alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate for diuretic activity was validated, and triterpenes were the material basis for the diuretic activity of AR.

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Section: Introductionmentioning

confidence: 99%

Diuretic Activity of Compatible Triterpene Components of Alismatis rhizoma

Zhang

Lin

et al. 2017

Molecules

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“…(Alismataceae), which is an aquatic plant that has been cultivated mainly in oriental countries (e.g., China, Japan, and Korea) but is also widely distributed in other areas (e.g., North America and Europe). AR has various activities, such as diuretic, hyperlipidemic, inflammatory, antitumoral, and damp-heat clearing activities [ 2 – 8 ]. AR is traditional folk medicine that has long been used to promote health and longevity (Sheng Nong's herbal classic) for more than several thousand years.…”

Section: Introductionmentioning

confidence: 99%

Alismatis Rhizoma Triterpenes Alleviate High-Fat Diet-Induced Insulin Resistance in Skeletal Muscle of Mice

Jia

Huang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Alismatis rhizoma (AR), which is the dried rhizome of Alisma orientale (Sam.) Juz. (Alismataceae), is an important component of many famous Chinese formulas for hypoglycemic. This study aimed to evaluate the insulin resistance (IR) alleviating effects of AR triterpenes (ART) and ART component compatibility (ARTC, the mixture of 16-oxo-alisol A, 16-oxo-alisol A 23-acetate, 16-oxo-alisol A 24-acetate, alisol C, alisol C 23-acetate, alisol L, alisol A, alisol A 23-acetate, alisol A 24-acetate, alisol L 23-acetate, alisol B, alisol B 23-acetate, 11-deoxy-alisol B and 11-deoxy-alisol B 23-acetate) in high-fat diet-induced IR mice and plamitate-treated IR C2C12 cells, respectively. A dose of 200 mg/kg of ART was orally administered to IR mice, and different doses (25, 50, and 100 μg/ml) of ARTC groups were treated to IR C2C12 cells. IPGTT, IPITT, body weight, Hb1AC, FFA, TNF- α , MCP-1, and IR-associated gene expression (p-AMPK, p-IRS-1, PI3K, p-AKT, p-JNK, and GLUT4) were measured in IR mice. Glucose uptake, TNF- α , MCP-1, and IR-associated gene expression were also measured in IR C2C12 cells. Results showed that ART alleviated high-fat diet-induced IR in the skeletal muscle of mice, and this finding was further validated by ARTC. This study demonstrated that ART presented a notable IR alleviating effect by regulating IR-associated gene expression, and triterpenes were the material basis for the IR alleviating activity of AR.

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“…It is commonly used to treat di erent diseases, including cancer and induced autophagy-dependent cell death through the accumulation of intracellular ROS and subsequent activation of JNK signaling pathway in human colon cancer (Zhao et al 2017). In human renal proximal tubular cells, AB23A-induced cell apoptosis is mediated by autophagy and nephrotoxicity via PI3K/AKT/mTOR signaling pathway (Wang et al 2017). erefore, more and more attention has been paid to AB23A because of its potential anti-cancer e ect and the research value in the treatment of human cancer.…”

Section: Introductionmentioning

confidence: 99%

The effects of Alisol B 23-acetate in hepatocellular carcinoma via inducing cell apoptosis and inhibiting cell migration and invasion

Li¹,

Cheng²,

Zhu³

et al. 2020

gpb

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Alisol B 23-acetate (AB23A) is a natural triterpenoid isolated from Chinese herbal medicine and has a variety of biological functions, especially anti-cancer e ects. However, the e ects and mechanisms of AB23A in hepatocellular carcinoma (HCC) remain unclear. Cell viability, invasion and migration were measured by MTT, Transwell and wound healing assays, respectively. To detect cell cycle and apoptosis, a ow cytometry assay was used. Tumor xenogra experiment was performed to measure tumor growth. e enzymatic assay was used to determine the activity of matrix metalloproteinase (MMP)-2 and-9. Furthermore, the mRNA and protein levels of Bcl-2, Bax, Caspase-3/-9, MMP-2/-9 and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) were detected by RT-PCR and Western blotting assays. AB23A suppressed cell viability in a concentration-dependent manner, blocked cell cycle, and induced apoptosis via up-regulating Bax, Caspase-3 and Caspase-9, and down-regulating Bcl-2 in HCC cells both in vitro and in vivo. In addition, AB23A inhibited cell invasion and migration through down-regulating MMP-2/-9 activities. e e ects of AB23A might be associated with the PI3K/Akt signaling pathway in HCC cells. Taken together, the present data demonstrated that AB23A might play a role in suppressing the progression of HCC, revealing the value of AB23A for hepatocellular carcinoma treatment in clinic.

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Alisol A 24-Acetate and Alisol B 23-Acetate Induced Autophagy Mediates Apoptosis and Nephrotoxicity in Human Renal Proximal Tubular Cells

Cited by 10 publications

References 46 publications

Diuretic Activity of Compatible Triterpene Components of Alismatis rhizoma

Diuretic Activity of Compatible Triterpene Components of Alismatis rhizoma

Alismatis Rhizoma Triterpenes Alleviate High-Fat Diet-Induced Insulin Resistance in Skeletal Muscle of Mice

The effects of Alisol B 23-acetate in hepatocellular carcinoma via inducing cell apoptosis and inhibiting cell migration and invasion

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