Aliphatic polyamines in physiology and diseases

Ramani, David; Bandt, J.-P. De; Cynober, Luc

doi:10.1016/j.clnu.2013.09.019

Cited by 112 publications

(86 citation statements)

References 46 publications

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“…When considering polyamine metabolism, on the other hand, their synthesis is known to involve two alternative pathways, via ornithine and agmatine, both leading to the formation of the diamine putrescine, with the other two polyamines deriving from the subsequent attachment of two propylamine groups to putrescine by spermidine and spermine synthetase. However, the role of agmatine in polyamine metabolism in extra nervous mammalian tissues is still under debate, although well-characterized in plants, bacteria, fungi and invertebrates [12]. Therefore, the main factor in determining tissue polyamine levels remains undoubtedly the activity of ornithine decarboxylase (ODC), the rate limiting enzyme in polyamine synthesis from ornithine [10].…”

Section: Introductionmentioning

confidence: 99%

Gliadin-mediated production of polyamines by RAW264.7 macrophages modulates intestinal epithelial permeability in vitro

Barilli

Rotoli

Visigalli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Celiac disease (CD) is an immune-mediated enteropathy sustained by dietary gluten in susceptible individuals, and characterized by a complex interplay between adaptive and innate responses against gluten peptides (PTG). In a recent contribution we have demonstrated that the treatment with PTG induces the expression and activity of arginase in both murine macrophages and human monocytes from healthy subjects, thus suggesting a role for arginine and its metabolites in gluten-triggered response of these cells. Here we further explore this field, by addressing the effects of PTG on polyamine synthesis and release in murine RAW264.7 macrophages, and how they affect epithelial permeability of Caco-2 monolayers. Results obtained show a massive production and release of putrescine by macrophages upon incubation with gluten peptides; this, in turn, causes a decrease in TEER in epithelial cells, indicating that PTG-driven secretion of polyamines by macrophages has a role in the modulation of intestinal permeability in vitro. At a molecular level, putrescine production appears referable to the activation of C/EBPβ transcription factor, which is known to be responsible for arginase induction in activated macrophages and is a crucial mediator of inflammation. Whether these pathways are stimulated also in vivo deserves to be further investigated, as well as their role in gluten-driven cellular and intestinal defects typical of CD patients.

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Section: Introductionmentioning

confidence: 99%

Gliadin-mediated production of polyamines by RAW264.7 macrophages modulates intestinal epithelial permeability in vitro

Barilli

Rotoli

Visigalli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…Bound polyamines are in equilibrium with the total free cellular polyamine pool that makes up 7-10% of the cell content. Among the three polyamines, spermine appears the most active because it contains the most charges (four) while putrescine contains the fewest (two) [12, 13]. …”

Section: Introductionmentioning

confidence: 99%

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects

Wang

Stearns

et al. 2014

Clinical Immunology

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Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE). To elucidate specificity further, the effect of polyamines on the binding of anti-DNA antibodies from patients with lupus was tested by ELISA to calf thymus (CT) DNA; we also assessed the binding of plasmas of patients and normal human subjects (NHS) to Micrococcus luteus (MC) DNA. As these studies showed, spermine can dose-dependently inhibit SLE anti-DNA binding to CT DNA and can promote dissociation of preformed immune complexes. With MC DNA as antigen, spermine failed to inhibit the NHS anti-DNA binding. Studies using plasmas adsorbed to a CT DNA cellulose affinity indicated that SLE plasmas are mixtures of anti-DNA that differ in inhibition by spermine and binding to conserved and non-conserved determinants. Together, these studies demonstrate that spermine can influence the binding of anti-DNA autoantibodies and may contribute to the antigenicity of DNA.

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“…They have pleiotropic effects on cell physiology and play a relevant role in cell proliferation and differentiation (3). These molecules are positively charged at the primary and secondary amino groups at physiological ph.…”

Section: Polyamine Metabolism and Colorectal Cancermentioning

confidence: 99%

Pharmacological and dietary agents for colorectal cancer chemoprevention: Effects on polyamine metabolism (Review)

Linsalata¹,

Orlando²,

Russo³

2014

International Journal of Oncology

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Abstract. Chemoprevention is the long-term use of different chemical agents, both synthetic and natural, to prevent or delay the onset of disease. Since colorectal cancer has a significant environmental component, it is an ideal disease in which to evaluate the potential benefits of chemopreventive agents. The polyamines, spermine, spermidine and putrescine have been involved in almost all the steps of colorectal tumorigenesis. Consequently, polyamine biosynthesis and catabolism can be considered as promising targets for cancer chemoprevention. A variety of drug formulations have been tested for their efficacy in affecting polyamines in a strategy of colorectal cancer prevention. Different molecules, such as biosynthesis inhibitors and catabolism inducers, have been proposed alone or in combination with other drugs proved to diminish the colorectal cancer risk. Interestingly, also diet can play a role in cancer prevention by affecting polyamines. Several dietary components, such as probiotics or flavonoids, have been shown to affect the polyamine metabolic pathway in colorectal neoplastic tissue. On the other hand, the polyamines ingested with diet might contrast the above cited effects shown by both drugs and nutritional factors. It is, therefore, fundamental to acquire more data also on these aspects in view of an innovative approach to colorectal oncology. This review summarizes data on the role of polyamine metabolism in neoplastic transformation of colorectal mucosa and as possible target for colorectal cancer chemoprevention. Attention will be focused on the influence of drugs and nutritional factors on polyamine metabolism, as well as the role played by dietary polyamines.

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Aliphatic polyamines in physiology and diseases

Cited by 112 publications

References 46 publications

Gliadin-mediated production of polyamines by RAW264.7 macrophages modulates intestinal epithelial permeability in vitro

Gliadin-mediated production of polyamines by RAW264.7 macrophages modulates intestinal epithelial permeability in vitro

The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects

Pharmacological and dietary agents for colorectal cancer chemoprevention: Effects on polyamine metabolism (Review)

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