Aliphatic and eudesmalolide esters extracted from the roots of Inula racemosa Hook

Khan, M. S. Y.; Mir, Showkat R.; Ali, Abuzar; Yusuf, Mohammed; Ali, Mohammed

doi:10.4103/0973-1296.126657

Cited by 6 publications

(1 citation statement)

References 10 publications

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“…These secondary metabolites include eudesmulolide esters (Khan et al. 2014 ), isoalantolactone, dihydroisoalantolactone, alantodiene, isoalantodiene (Sharma et al. 2016 ), sesquiterpenoids (Zhang et al.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and molecular docking analysis of racemolactone I, a new sesquiterpene lactone isolated from Inula racemosa

Alam

Tyagi

Farah

et al. 2021

Pharmaceutical Biology

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Context: Traditionally, Inula racemosa Hook. f. (Asteraceae) has been reported to be effective in cancer treatment which motivated the authors to explore the plant for novel anticancer compounds. Objective: To isolate and characterize new cytotoxic phytoconstituents from I. racemosa roots. Materials and methods: The column chromatography of I. racemosa ethyl acetate extract furnished a novel sesquiterpene lactone whose structure was established by NMR (1D/2D), ES-MS and its cytotoxic properties were assessed on HeLa, MDAMB-231, and A549 cell lines using MTT and LDH (lactate dehydrogenase) assays. Further, morphological changes were analyzed by flow cytometry, mitochondrial membrane potential, AO-EtBr dual staining, and comet assay. Molecular docking and simulation were performed using Glide and Desmond softwares, respectively, to validate the mechanism of action. Results: The isolated compound was identified as racemolactone I (compound 1). Amongst the cell lines tested, considerable changes were observed in HeLa cells. Compound 1 (IC 50 ¼ 0.9 mg/mL) significantly decreased cell viability (82%) concomitantly with high LDH release (76%) at 15 mg/mL. Diverse morphological alterations along with significant increase (9.23%) in apoptotic cells and decrease in viable cells were observed. AO-EtBr dual staining also confirmed the presence of 20% apoptotic cells. A gradual decrease in mitochondrial membrane potential was observed. HeLa cells showed significantly increased comet tail length (48.4 mm), indicating broken DNA strands. In silico studies exhibited that compound 1 binds to the active site of Polo-like kinase-1 and forms a stable complex. Conclusions: Racemolactone I was identified as potential anticancer agent, which can further be confirmed by in vivo investigations.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity and molecular docking analysis of racemolactone I, a new sesquiterpene lactone isolated from Inula racemosa

Alam

Tyagi

Farah

et al. 2021

Pharmaceutical Biology

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Phytochemical Diversity in Relation to Cytogenetic Variability in Inula racemosa Hook.f., an Endangered Medicinal Plant of Himalayas

Jeelani

Shahnawaza

Gupta

et al. 2022

Chemistry & Biodiversity

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Inula racemosa, a resourceful critically endangered medicinal herb in the Himalayas is traditionally utilized to cure various human disorders. The species is a wealthy source of sesquiterpene lactones has many pharmacological properties. To quantify and identify the best genetic stocks for a maximum build‐up of desired metabolites (isoalantolactone and alantolactone) among existent cytotypes in the species, LC‐MS/MS analysis was made. The other comprehensive experiments carried out at present included detailed meiotic examinations of different populations collected from different areas of Kashmir Himalayas. The results presented the occurrence of variable chromosome numbers as n=10 and n=20 in different populations, but the tetraploid cytotype (n=20) is new for the species. The LC‐MS/MS investigation revealed significant variability in the content of sesquiterpene lactones in different plant tissues (stem, leaf, and root). An upsurge in the quantity of isoalantolactone and alantolactone was noticed with increasing ploidy levels along the increasing altitudes. Therefore, a habit to accumulate abundant quantities of secondary metabolites and increased adaptability by species/cytotypes thriving at higher altitudes is seen among tetraploid cytotypes during the present investigation. Also, the chromosomal variations seem to enhance the flexibility of polyploid species primarily at upper elevations. Thus, the present study strongly provides quantification of elite cytotypes/chemotypes with optimum concentration of secondary metabolites.

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Inula racemosa Hook. f. Pushkarmool: Its Ethnobotanical Uses, Phytochemicals, and Pharmacological Activities

Wangchuk

Jamtsho

2023

Immunity Boosting Medicinal Plants of the Western Himalayas

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Aliphatic and eudesmalolide esters extracted from the roots of Inula racemosa Hook

Cited by 6 publications

References 10 publications

Cytotoxicity and molecular docking analysis of racemolactone I, a new sesquiterpene lactone isolated from Inula racemosa

Cytotoxicity and molecular docking analysis of racemolactone I, a new sesquiterpene lactone isolated from Inula racemosa

Phytochemical Diversity in Relation to Cytogenetic Variability in Inula racemosa Hook.f., an Endangered Medicinal Plant of Himalayas

Inula racemosa Hook. f. Pushkarmool: Its Ethnobotanical Uses, Phytochemicals, and Pharmacological Activities

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